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The map label for this gene is lipB [H]

Identifier: 188584792

GI number: 188584792

Start: 175696

End: 176457

Strand: Reverse

Name: lipB [H]

Synonym: Nther_0150

Alternate gene names: 188584792

Gene position: 176457-175696 (Counterclockwise)

Preceding gene: 188584794

Following gene: 188584790

Centisome position: 5.57

GC content: 37.66

Gene sequence:

>762_bases
ATGAAAGTAACATTCTTAGGTACAGGGCCATCTCACGGGGTCCCGGTAATTGGTTGTAGCTGTTCAGTGTGTTCAAGTAA
AGATTCAAAAAACACCAGGTACCGGTCGAGCGTGATCATCAAATATGAAAATAATCACCTGTTAATTGATACTCCACCGG
AGTTTCGGTTGCAAATGATCAATAACAATATACACAGGATAGATGGAGTGCTTTTTACCCATCCCCATGCTGACCATGTT
CACGGTTTTGACGATTTAAGACGCTTCAATGAAATTCAAAGGGAATCTATTCCTTGTTTTGCCAGTTCAGAAACTGTAAA
GAATCTAAAAAACATGTATTCTTATGTCTTTAGAGGTGGAGATCCCTATACTAGTAGTCCCAAAGTTACTTTACATTCAA
TCTCGAGTAGTTTTGAACTCAATGGCTTGACCATCAACCCAATTCCAATATATCATGGGAAGTCTTTAATCCTGGGTTAT
CGTATCGGTAGGTTCGCTTATCTTACAGATTGTTCTCAGATTCCTCCCGATTCATATAAATTTTTACAGGGTCTGCATAC
TCTAGTGATCGGTGCTTTGAGGTATCGTTCTCACCCTAACCATTTAAGTGTTGATGAAGCTGTAGAGACTGTAAATAAAA
TAAATCCTGAAGTTGCTTACTTTACACATATGACTCATGATCTAGACTACTATCAGTTAAATGAACAATTACCAGTTAAT
ATAAAACCGGCCTTTGATAATTTAACTATCCAAGTCGAATGA

Upstream 100 bases:

>100_bases
ACAATAGTTTTTCTTTTAGATCTTGAAATTTTTTGTTATCAGCCATTAATAGGCCTCCTTTCGCAGTAATTAGTATTAAA
AGAAAATGAGGGATGAATAT

Downstream 100 bases:

>100_bases
TAAAATTAAGAAAATAACAAAGTGTCAAAAAGAGGCAGTTTAAGCTGCCTCTTTTATTTGAAATATCTATTAACTAGCTA
CATTTAGAGTATCCACAATA

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 253; Mature: 253

Protein sequence:

>253_residues
MKVTFLGTGPSHGVPVIGCSCSVCSSKDSKNTRYRSSVIIKYENNHLLIDTPPEFRLQMINNNIHRIDGVLFTHPHADHV
HGFDDLRRFNEIQRESIPCFASSETVKNLKNMYSYVFRGGDPYTSSPKVTLHSISSSFELNGLTINPIPIYHGKSLILGY
RIGRFAYLTDCSQIPPDSYKFLQGLHTLVIGALRYRSHPNHLSVDEAVETVNKINPEVAYFTHMTHDLDYYQLNEQLPVN
IKPAFDNLTIQVE

Sequences:

>Translated_253_residues
MKVTFLGTGPSHGVPVIGCSCSVCSSKDSKNTRYRSSVIIKYENNHLLIDTPPEFRLQMINNNIHRIDGVLFTHPHADHV
HGFDDLRRFNEIQRESIPCFASSETVKNLKNMYSYVFRGGDPYTSSPKVTLHSISSSFELNGLTINPIPIYHGKSLILGY
RIGRFAYLTDCSQIPPDSYKFLQGLHTLVIGALRYRSHPNHLSVDEAVETVNKINPEVAYFTHMTHDLDYYQLNEQLPVN
IKPAFDNLTIQVE
>Mature_253_residues
MKVTFLGTGPSHGVPVIGCSCSVCSSKDSKNTRYRSSVIIKYENNHLLIDTPPEFRLQMINNNIHRIDGVLFTHPHADHV
HGFDDLRRFNEIQRESIPCFASSETVKNLKNMYSYVFRGGDPYTSSPKVTLHSISSSFELNGLTINPIPIYHGKSLILGY
RIGRFAYLTDCSQIPPDSYKFLQGLHTLVIGALRYRSHPNHLSVDEAVETVNKINPEVAYFTHMTHDLDYYQLNEQLPVN
IKPAFDNLTIQVE

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=258, Percent_Identity=25.5813953488372, Blast_Score=74, Evalue=9e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 28711; Mature: 28711

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS00626 RCC1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVTFLGTGPSHGVPVIGCSCSVCSSKDSKNTRYRSSVIIKYENNHLLIDTPPEFRLQMI
CEEEEEECCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCHHHHHHHH
NNNIHRIDGVLFTHPHADHVHGFDDLRRFNEIQRESIPCFASSETVKNLKNMYSYVFRGG
CCCCCHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCC
DPYTSSPKVTLHSISSSFELNGLTINPIPIYHGKSLILGYRIGRFAYLTDCSQIPPDSYK
CCCCCCCCEEEEECCCCEEECCEEECCEEEECCCEEEEEEECCCEEEEECCCCCCCCHHH
FLQGLHTLVIGALRYRSHPNHLSVDEAVETVNKINPEVAYFTHMTHDLDYYQLNEQLPVN
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCEE
IKPAFDNLTIQVE
EEECCCCEEEEEC
>Mature Secondary Structure
MKVTFLGTGPSHGVPVIGCSCSVCSSKDSKNTRYRSSVIIKYENNHLLIDTPPEFRLQMI
CEEEEEECCCCCCCEEEECCCCCCCCCCCCCCEEEEEEEEEECCCEEEEECCHHHHHHHH
NNNIHRIDGVLFTHPHADHVHGFDDLRRFNEIQRESIPCFASSETVKNLKNMYSYVFRGG
CCCCCHHCCEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEECHHHHHHHHHHHHHHHCCC
DPYTSSPKVTLHSISSSFELNGLTINPIPIYHGKSLILGYRIGRFAYLTDCSQIPPDSYK
CCCCCCCCEEEEECCCCEEECCEEECCEEEECCCEEEEEEECCCEEEEECCCCCCCCHHH
FLQGLHTLVIGALRYRSHPNHLSVDEAVETVNKINPEVAYFTHMTHDLDYYQLNEQLPVN
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCEEEEEEECCCCCEEEECCCCCEE
IKPAFDNLTIQVE
EEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]