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The map label for this gene is yciL [C]

Identifier: 188528230

GI number: 188528230

Start: 1447661

End: 1448434

Strand: Reverse

Name: yciL [C]

Synonym: HPSH_07470

Alternate gene names: 188528230

Gene position: 1448434-1447661 (Counterclockwise)

Preceding gene: 188528231

Following gene: 188528229

Centisome position: 90.05

GC content: 40.83

Gene sequence:

>774_bases
TTGAGGATCAACCAGTTTTTAGCCCATTACACCAAGCATTCAAGAAGAGAAGCTGAAAAATTGGTTTTAGAAGGGCGGGT
AAAAATCAATCATGAGCATGCCAAACTCGCTAGCGTGGTTAAAGAAAACGACAAGGTGTTTTTAGACAAACGACTCATCA
AGCCCTTAAAAAACAAAAAATTCAGCGTGCTTGTCTATCACAAGCCAAAGGGCGAATTGGTGAGTAAAAACGATCCCTTA
AAACGGCGCGTGATTTATGAAAGCTTGGAAAAAAAATACGCCCATTTTGCGCCTGTGGGGCGTTTGGATTTTGCGAGTGA
AGGGGTGCTATTATTGAGCGATAGTAAGGCGGTGGTGAGCGCTTTAATGCATGCGAATTTAGAAAAAGAATATCTTGTTA
AAATTCAAGGCTTTGTTACAAGAGAGATGGAAAACGCTATGCAAGAGGGCTTGAAATTAGAAAACGCTACTAAGGGAGCG
CACCAAAAAACCCCCATTAAAAGCATGGAATTTGCCCCCTTTATTGGTTATGAAATCATCAAAAACCATGCCAAATACTC
TAAACTTAGAGTCATTATCAATGAGGGGAAGAACAGAGAATTGAGGCGCTTTTTTGCGTTTTTTAACGCTGGAGTGTTGG
ATTTAAGGCGCGTTCGTTATGGTTTTGTGAATTTGAATGCCTTGCCGGTGGGGAAAATGCGTTTTCTAAACCGCCAAGAA
TACAACGAGTTGCATGCGTTTATGGCTAATGCGGCTAATGTTAAAGAGGATTAA

Upstream 100 bases:

>100_bases
TCATCAAAGACAAGGATAAGCAATTCAAAATCCAAAGCGATTTAATGGTGAATGAAAAGATTAAGGACGATTTTAAAGGG
TTAGAGTGGAGGGATTTAGC

Downstream 100 bases:

>100_bases
CTTACGAGTTATGCTATACTTTGGGGTTGGGGGAAATAAAAAATAAGGATTATCATGTCAGAAATTATTAACGGGAAGAA
TTACGCAGAGAAAATCGCTC

Product: hypothetical protein

Products: NA

Alternate protein names: RNA pseudouridylate synthase; RNA-uridine isomerase [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRVIYESLEKKYAHFAPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLVKIQGFVTREMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKLRVIINEGKNRELRRFFAFFNAGVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANAANVKED

Sequences:

>Translated_257_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRVIYESLEKKYAHFAPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLVKIQGFVTREMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKLRVIINEGKNRELRRFFAFFNAGVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANAANVKED
>Mature_257_residues
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKKFSVLVYHKPKGELVSKNDPL
KRRVIYESLEKKYAHFAPVGRLDFASEGVLLLSDSKAVVSALMHANLEKEYLVKIQGFVTREMENAMQEGLKLENATKGA
HQKTPIKSMEFAPFIGYEIIKNHAKYSKLRVIINEGKNRELRRFFAFFNAGVLDLRRVRYGFVNLNALPVGKMRFLNRQE
YNELHAFMANAANVKED

Specific function: Unknown

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787524, Length=255, Percent_Identity=30.9803921568627, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1790453, Length=255, Percent_Identity=28.2352941176471, Blast_Score=96, Evalue=2e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 29767; Mature: 29767

Theoretical pI: Translated: 10.57; Mature: 10.57

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKK
CCHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCEEHHHHHHHHHCCCC
FSVLVYHKPKGELVSKNDPLKRRVIYESLEKKYAHFAPVGRLDFASEGVLLLSDSKAVVS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHH
ALMHANLEKEYLVKIQGFVTREMENAMQEGLKLENATKGAHQKTPIKSMEFAPFIGYEII
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KNHAKYSKLRVIINEGKNRELRRFFAFFNAGVLDLRRVRYGFVNLNALPVGKMRFLNRQE
HHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCCHHHHHHHHHH
YNELHAFMANAANVKED
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MRINQFLAHYTKHSRREAEKLVLEGRVKINHEHAKLASVVKENDKVFLDKRLIKPLKNKK
CCHHHHHHHHHHHHHHHHHHHHHHCCEEECHHHHHHHHHHHCCCCEEHHHHHHHHHCCCC
FSVLVYHKPKGELVSKNDPLKRRVIYESLEKKYAHFAPVGRLDFASEGVLLLSDSKAVVS
EEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHH
ALMHANLEKEYLVKIQGFVTREMENAMQEGLKLENATKGAHQKTPIKSMEFAPFIGYEII
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
KNHAKYSKLRVIINEGKNRELRRFFAFFNAGVLDLRRVRYGFVNLNALPVGKMRFLNRQE
HHHHHHHHEEEEEECCCCHHHHHHHHHHCCCHHHHHHHHHCEEEECCCCCHHHHHHHHHH
YNELHAFMANAANVKED
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]