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The map label for this gene is trmE

Identifier: 188528222

GI number: 188528222

Start: 1440184

End: 1441536

Strand: Direct

Name: trmE

Synonym: HPSH_07430

Alternate gene names: 188528222

Gene position: 1440184-1441536 (Clockwise)

Preceding gene: 188528221

Following gene: 188528223

Centisome position: 89.53

GC content: 38.95

Gene sequence:

>1353_bases
ATGAATGACACCATCGCCGCTATCGCTACCCCTTTAGGCAAGGGAGCGATTAGCGTCATTAAAATCAGTGGCAATAGCGC
TCTAAATATTCTCAAACAACTCACCCAAAAACAAGACTTCACCCCCAGATACGCTTATGTGTGCGATATTTTTTCTAATG
GTGTTTTATTGGACAAAGCGTTAGTGATTTATTTCAAAGCCCCCTATAGTTTCACTGGTGAAGATGTGTGCGAAATCCAA
TGCCATGGAAACCCCCTTTTAGCGCAAAATATCTTACAAGCTTGTTTGAATTTAGGGGCTAGGCTTGCTAAAGCGGGGGA
ATTTAGCAAAAAAGCCTTTTTAAACCATAAAATGGATTTGAGCGAGATTGAAGCGAGCGTTCAGCTCATCCTTTGTGAAG
ATGAAAGCGTTTTAAACGCTCTAGCCAGGCAACTTAAGGGCGAGTTAAAAATTTTTATTGAAGAGGCTAGAGGCAATCTT
TTAAAGCTTTTAGCGAGCTCAGAAGTTTTGATTGATTATAGCGAAGAAGACATCCCTAGCGATTTTTTAGATGAAGTATC
TCTTAATTTAGAAAAGCAAATCGCCTCTTTTAAAGACTTACTGGATTTTTCTAACACGCAAAAACAAAAAAACAAAGGGC
ATGCCTTAAGCATTGTTGGCAAACCCAATGCTGGCAAAAGCTCCCTATTAAACGCCATGCTTTTAGAAGAAAGGGCTTTA
GTGAGCGATATTAAAGGCACAACAAGAGACACCATAGAAGAAGTCATTGAATTGCAAGGGCATAAGGTGCGCTTGATTGA
TACGGCTGGCATTAGAGAGAGCGCGGATAAAATAGAGCGATTAGGGATTGAAAAAAGCCTTAAAAGTTTAGAAAATTGCG
ACATTATTTTAGGCGTGTTTGATCTTTCTAAACCCCTAGAAAAAGAAGATTTTAACCTTATTGACACCCTCAATCGCGCT
AAAAAGCCTTGCATTGTTGTTTTGAATAAAAACGATTTAGCGCCCAAACTGGAGCTTGAAATCTTAAAATCCCATCTTAA
AATCCCTTATTCTTTACTAGAAACCAACACCCTAAATTCCAAGGCTTGTTTGAAAGATTTGAGCCAAAAAATCAGCGCCT
TTTTCCCTAAACTAGACACTCAAAACAAGCTCTTACTCACTTCCCTAGCCCAAAAAACCGCCCTAGAAAACGCCATTTTT
GAATTGCAAAACGCTAAAAACCATTTAGAAACTTTAGAGCTTTTTTCTTATCATCTTTTAAGCGCGATAGAAAGCTTGAA
CTTGCTCACCCGTCCTTATGAAACCAGCCAAATGCTAGACAGCATGTTTAGCGAATTTTGCTTAGGCAAATGA

Upstream 100 bases:

>100_bases
GCTTTAAAGAAATTACGAAAAGCGTTCCCTAACAAATATGTTTCCATCAAAACCAATTTAAACGATGAAAAATACATCGT
CATCAACGATTTCCACCACA

Downstream 100 bases:

>100_bases
AACCGCTTAAAGAATGGGTTAAAACCCCTTTTTAGCGCCACTTTTTAAGACTTTTTACCCATTAGCGCCGTTTAATATCT
ATATTTACAAAAAAACAATA

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MNDTIAAIATPLGKGAISVIKISGNSALNILKQLTQKQDFTPRYAYVCDIFSNGVLLDKALVIYFKAPYSFTGEDVCEIQ
CHGNPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGELKIFIEEARGNL
LKLLASSEVLIDYSEEDIPSDFLDEVSLNLEKQIASFKDLLDFSNTQKQKNKGHALSIVGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKEDFNLIDTLNRA
KKPCIVVLNKNDLAPKLELEILKSHLKIPYSLLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKTALENAIF
ELQNAKNHLETLELFSYHLLSAIESLNLLTRPYETSQMLDSMFSEFCLGK

Sequences:

>Translated_450_residues
MNDTIAAIATPLGKGAISVIKISGNSALNILKQLTQKQDFTPRYAYVCDIFSNGVLLDKALVIYFKAPYSFTGEDVCEIQ
CHGNPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGELKIFIEEARGNL
LKLLASSEVLIDYSEEDIPSDFLDEVSLNLEKQIASFKDLLDFSNTQKQKNKGHALSIVGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKEDFNLIDTLNRA
KKPCIVVLNKNDLAPKLELEILKSHLKIPYSLLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKTALENAIF
ELQNAKNHLETLELFSYHLLSAIESLNLLTRPYETSQMLDSMFSEFCLGK
>Mature_450_residues
MNDTIAAIATPLGKGAISVIKISGNSALNILKQLTQKQDFTPRYAYVCDIFSNGVLLDKALVIYFKAPYSFTGEDVCEIQ
CHGNPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDLSEIEASVQLILCEDESVLNALARQLKGELKIFIEEARGNL
LKLLASSEVLIDYSEEDIPSDFLDEVSLNLEKQIASFKDLLDFSNTQKQKNKGHALSIVGKPNAGKSSLLNAMLLEERAL
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVFDLSKPLEKEDFNLIDTLNRA
KKPCIVVLNKNDLAPKLELEILKSHLKIPYSLLETNTLNSKACLKDLSQKISAFFPKLDTQNKLLLTSLAQKTALENAIF
ELQNAKNHLETLELFSYHLLSAIESLNLLTRPYETSQMLDSMFSEFCLGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34 [H]

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain [H]

Homologues:

Organism=Homo sapiens, GI193082991, Length=466, Percent_Identity=33.2618025751073, Blast_Score=199, Evalue=6e-51,
Organism=Homo sapiens, GI193082993, Length=457, Percent_Identity=32.3851203501094, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI193082988, Length=498, Percent_Identity=31.1244979919679, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI2367268, Length=464, Percent_Identity=34.2672413793103, Blast_Score=222, Evalue=3e-59,
Organism=Escherichia coli, GI87082120, Length=120, Percent_Identity=37.5, Blast_Score=92, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=459, Percent_Identity=29.6296296296296, Blast_Score=188, Evalue=6e-48,
Organism=Saccharomyces cerevisiae, GI6323665, Length=490, Percent_Identity=32.2448979591837, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550813, Length=304, Percent_Identity=33.2236842105263, Blast_Score=154, Evalue=1e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018948
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520 [H]

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N [H]

EC number: NA

Molecular weight: Translated: 49988; Mature: 49988

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTIAAIATPLGKGAISVIKISGNSALNILKQLTQKQDFTPRYAYVCDIFSNGVLLDKA
CCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECE
LVIYFKAPYSFTGEDVCEIQCHGNPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDL
EEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
SEIEASVQLILCEDESVLNALARQLKGELKIFIEEARGNLLKLLASSEVLIDYSEEDIPS
HHHHCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCH
DFLDEVSLNLEKQIASFKDLLDFSNTQKQKNKGHALSIVGKPNAGKSSLLNAMLLEERAL
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVF
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
DLSKPLEKEDFNLIDTLNRAKKPCIVVLNKNDLAPKLELEILKSHLKIPYSLLETNTLNS
CCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCH
KACLKDLSQKISAFFPKLDTQNKLLLTSLAQKTALENAIFELQNAKNHLETLELFSYHLL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIESLNLLTRPYETSQMLDSMFSEFCLGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDTIAAIATPLGKGAISVIKISGNSALNILKQLTQKQDFTPRYAYVCDIFSNGVLLDKA
CCCCHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEECE
LVIYFKAPYSFTGEDVCEIQCHGNPLLAQNILQACLNLGARLAKAGEFSKKAFLNHKMDL
EEEEEECCCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCH
SEIEASVQLILCEDESVLNALARQLKGELKIFIEEARGNLLKLLASSEVLIDYSEEDIPS
HHHHCEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHCCCEEEECCCCCCCH
DFLDEVSLNLEKQIASFKDLLDFSNTQKQKNKGHALSIVGKPNAGKSSLLNAMLLEERAL
HHHHHHHCCHHHHHHHHHHHHHCCCHHHHCCCCCEEEEEECCCCCHHHHHHHHHHHHHHH
VSDIKGTTRDTIEEVIELQGHKVRLIDTAGIRESADKIERLGIEKSLKSLENCDIILGVF
HHHHCCCCHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEEE
DLSKPLEKEDFNLIDTLNRAKKPCIVVLNKNDLAPKLELEILKSHLKIPYSLLETNTLNS
CCCCCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCHHHHHCCCCCH
KACLKDLSQKISAFFPKLDTQNKLLLTSLAQKTALENAIFELQNAKNHLETLELFSYHLL
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SAIESLNLLTRPYETSQMLDSMFSEFCLGK
HHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA