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The map label for this gene is ecoRVM [H]

Identifier: 188528157

GI number: 188528157

Start: 1374652

End: 1375545

Strand: Direct

Name: ecoRVM [H]

Synonym: HPSH_07080

Alternate gene names: 188528157

Gene position: 1374652-1375545 (Clockwise)

Preceding gene: 188528156

Following gene: 188528158

Centisome position: 85.46

GC content: 33.45

Gene sequence:

>894_bases
ATGATGGAAACACTGATCCCACCCATAAAATCCCAAGGTATTAAGACCAAATTAGTAGATTGGATTAAGGGGGTTTCAAA
AAAAGTTGCATTTGAGCGTTGGGTTGAGCCGTTTATGGGAACGGGCGTTGTGGCTTTTAATATCCAGCCTAAAAGAGCGT
TATTGTGCGATAGCAATCCGCATTTAATCCATTTCTATCAAGCCATTCAAAATGAAAAGATCACCCCAAATATTGTGAGA
GAATTTCTCAACGAAGAAGGTTTGAAATTATTGCAAAGCAATGGAGAATATTACTATGAAGTGAGGGAACGATTCAATAA
AGATTGTGATCCTCTTGATTTTCTATTCTTAAATCGTGCGTGTTTTAATGGCATGATACGCTTCAATCAAAAAGGGAAAT
TCAATGTGCCGTTTTGTAAAAAACCGCAGCGTTTTGCCCAAAGCTTGGTTACTAAGATTGTCAATCAAGTAGAAAATACA
GCAAAAATGATTAAAAACAATCATTATGAATTTAAATGTCAAGATTTCCAACAAACCATTTTAGAAACAAACAAAAGCGA
TTTAATTTATGCTGATCCCCCTTATATTGGAAGAAATGCAGATTATTTTGATTGCTGGAATGAAGAAAATGAAGTTACAC
TGCAACGAGTATTGTCAAAATCCAGGTGCAATTTCATTCTTTCTACATGGTTTAGCAATAAATATCGTAAAAATGATTAT
ATTTTTTCTATTTGGAAAAGTTACCCTATCGTAACTAAAGAGCATTTTTATCATGTTGGAGCTAAAGAAAATAATCGCAA
CATGATTTATGAAGCATTATTATCCAATATACAGATGAGCGATACAGATTTTAAGGAGCGTTATAATGAAAACGAACGAA
GTGCAATTTTATGA

Upstream 100 bases:

>100_bases
AAGGCAGTTTTAAAAAACTGACAAAATTAGCTGATTTCCTTGAATTTAAAGGAATTGATATTCAAAAAATCAATCCTATA
AAAAATAGGAGCAAGTCTTA

Downstream 100 bases:

>100_bases
AGTTTTAGAAAACCTTTTTATAGGCGTTAAGATTGAAGACAAACAAGAAAGCCTTTTAGATCCTAACGCTAAAGCAATGA
AAAATGGCATGCTCAATCTC

Product: adenine-specific DNA metylase

Products: NA

Alternate protein names: M.EcoRV; Adenine-specific methyltransferase EcoRV [H]

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMETLIPPIKSQGIKTKLVDWIKGVSKKVAFERWVEPFMGTGVVAFNIQPKRALLCDSNPHLIHFYQAIQNEKITPNIVR
EFLNEEGLKLLQSNGEYYYEVRERFNKDCDPLDFLFLNRACFNGMIRFNQKGKFNVPFCKKPQRFAQSLVTKIVNQVENT
AKMIKNNHYEFKCQDFQQTILETNKSDLIYADPPYIGRNADYFDCWNEENEVTLQRVLSKSRCNFILSTWFSNKYRKNDY
IFSIWKSYPIVTKEHFYHVGAKENNRNMIYEALLSNIQMSDTDFKERYNENERSAIL

Sequences:

>Translated_297_residues
MMETLIPPIKSQGIKTKLVDWIKGVSKKVAFERWVEPFMGTGVVAFNIQPKRALLCDSNPHLIHFYQAIQNEKITPNIVR
EFLNEEGLKLLQSNGEYYYEVRERFNKDCDPLDFLFLNRACFNGMIRFNQKGKFNVPFCKKPQRFAQSLVTKIVNQVENT
AKMIKNNHYEFKCQDFQQTILETNKSDLIYADPPYIGRNADYFDCWNEENEVTLQRVLSKSRCNFILSTWFSNKYRKNDY
IFSIWKSYPIVTKEHFYHVGAKENNRNMIYEALLSNIQMSDTDFKERYNENERSAIL
>Mature_297_residues
MMETLIPPIKSQGIKTKLVDWIKGVSKKVAFERWVEPFMGTGVVAFNIQPKRALLCDSNPHLIHFYQAIQNEKITPNIVR
EFLNEEGLKLLQSNGEYYYEVRERFNKDCDPLDFLFLNRACFNGMIRFNQKGKFNVPFCKKPQRFAQSLVTKIVNQVENT
AKMIKNNHYEFKCQDFQQTILETNKSDLIYADPPYIGRNADYFDCWNEENEVTLQRVLSKSRCNFILSTWFSNKYRKNDY
IFSIWKSYPIVTKEHFYHVGAKENNRNMIYEALLSNIQMSDTDFKERYNENERSAIL

Specific function: This methylase recognizes the double-stranded sequence GATATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the EcoRV endonuclease [H]

COG id: COG0338

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789789, Length=217, Percent_Identity=31.7972350230415, Blast_Score=88, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023095
- InterPro:   IPR002052
- InterPro:   IPR012327 [H]

Pfam domain/function: PF02086 MethyltransfD12 [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 35212; Mature: 35212

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMETLIPPIKSQGIKTKLVDWIKGVSKKVAFERWVEPFMGTGVVAFNIQPKRALLCDSNP
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCC
HLIHFYQAIQNEKITPNIVREFLNEEGLKLLQSNGEYYYEVRERFNKDCDPLDFLFLNRA
CEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHH
CFNGMIRFNQKGKFNVPFCKKPQRFAQSLVTKIVNQVENTAKMIKNNHYEFKCQDFQQTI
HHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
LETNKSDLIYADPPYIGRNADYFDCWNEENEVTLQRVLSKSRCNFILSTWFSNKYRKNDY
HHCCCCCEEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IFSIWKSYPIVTKEHFYHVGAKENNRNMIYEALLSNIQMSDTDFKERYNENERSAIL
EEEHHHCCCCEEHHHEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MMETLIPPIKSQGIKTKLVDWIKGVSKKVAFERWVEPFMGTGVVAFNIQPKRALLCDSNP
CCCCCCCCHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCEEEEECCCC
HLIHFYQAIQNEKITPNIVREFLNEEGLKLLQSNGEYYYEVRERFNKDCDPLDFLFLNRA
CEEHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHCCCCCHHHHHHHHHH
CFNGMIRFNQKGKFNVPFCKKPQRFAQSLVTKIVNQVENTAKMIKNNHYEFKCQDFQQTI
HHCCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHH
LETNKSDLIYADPPYIGRNADYFDCWNEENEVTLQRVLSKSRCNFILSTWFSNKYRKNDY
HHCCCCCEEEECCCCCCCCCCHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
IFSIWKSYPIVTKEHFYHVGAKENNRNMIYEALLSNIQMSDTDFKERYNENERSAIL
EEEHHHCCCCEEHHHEEECCCCCCCCHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6328432 [H]