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The map label for this gene is ecoRVR [H]

Identifier: 188528156

GI number: 188528156

Start: 1373810

End: 1374652

Strand: Direct

Name: ecoRVR [H]

Synonym: HPSH_07075

Alternate gene names: 188528156

Gene position: 1373810-1374652 (Clockwise)

Preceding gene: 188528130

Following gene: 188528157

Centisome position: 85.41

GC content: 30.13

Gene sequence:

>843_bases
ATGGAGTCAGTCAAACAAAATTTTATTGAAAAACTTAAGGTTTTTGCAACAGAATTAACTGACCATGTTACAACTCAGTT
GGGAGATTGGAAAATTAAAGGATTTATTGATATAGATAAAAATATTTATACAATTTCATCAGATACTAAAATTATTTCAA
AAATTCTTGAAATCCAACTTTTCCCTAAGTTTAAAGCATTTGCTAAGAAAAATGGGTATGAAATTATTATTGCTGAAAAA
CAAAATTGGTATCCTGATCTATCTTTTGTGTGCGAGAAAAATCCAAACATAAAGTTTGCTGTAGATATAAAAACAACTTA
TCGCTTAGATGATTGCTTGGGTTTTTGTAATGGTTTTACATTGGGTTCGCATGGTGAATATTTTAGGAATCGCGCTAGCA
CAAAAAATATCCAATTCCCCTATTCGCATTATCTGGCTCATATTTGTTTGGGTATTTTATACACACGATCTGCCTCTAGC
GGTATAGATGAAACAGAAATTTTACAATTAGAAAAGTTGGATAATATTACTTCTGTTATCAAAGATTTTATATTTTTTGC
TGAAGAGAAATGGAAAATCGCTAGCGATAAGGGCGGGAGTGGTAATACGGCTAATATTGGTAGCATTCAATGTATTGATG
ATATTTTGCAAGGGAATGGCATGTTTAAAAATCTTGGCGAACAAATTTTTGATGAATATTGGATCAATCAAGGCATGTTA
ATGATACCTGATTTAAAAAATCAAGGCAGTTTTAAAAAACTGACAAAATTAGCTGATTTCCTTGAATTTAAAGGAATTGA
TATTCAAAAAATCAATCCTATAAAAAATAGGAGCAAGTCTTAA

Upstream 100 bases:

>100_bases
TTGGTAAGGAGAAGCAAATTTTACAACACTAAAAGCGATATTTTTCGTGTTATAATCAGTTGGATATATTTGATAGCAAT
TGAGTGTTTGAAAGGTGGTT

Downstream 100 bases:

>100_bases
TGATGGAAACACTGATCCCACCCATAAAATCCCAAGGTATTAAGACCAAATTAGTAGATTGGATTAAGGGGGTTTCAAAA
AAAGTTGCATTTGAGCGTTG

Product: type II restriction endonuclease

Products: NA

Alternate protein names: R.EcoRV; Endonuclease EcoRV; Type II restriction enzyme EcoRV [H]

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MESVKQNFIEKLKVFATELTDHVTTQLGDWKIKGFIDIDKNIYTISSDTKIISKILEIQLFPKFKAFAKKNGYEIIIAEK
QNWYPDLSFVCEKNPNIKFAVDIKTTYRLDDCLGFCNGFTLGSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASS
GIDETEILQLEKLDNITSVIKDFIFFAEEKWKIASDKGGSGNTANIGSIQCIDDILQGNGMFKNLGEQIFDEYWINQGML
MIPDLKNQGSFKKLTKLADFLEFKGIDIQKINPIKNRSKS

Sequences:

>Translated_280_residues
MESVKQNFIEKLKVFATELTDHVTTQLGDWKIKGFIDIDKNIYTISSDTKIISKILEIQLFPKFKAFAKKNGYEIIIAEK
QNWYPDLSFVCEKNPNIKFAVDIKTTYRLDDCLGFCNGFTLGSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASS
GIDETEILQLEKLDNITSVIKDFIFFAEEKWKIASDKGGSGNTANIGSIQCIDDILQGNGMFKNLGEQIFDEYWINQGML
MIPDLKNQGSFKKLTKLADFLEFKGIDIQKINPIKNRSKS
>Mature_280_residues
MESVKQNFIEKLKVFATELTDHVTTQLGDWKIKGFIDIDKNIYTISSDTKIISKILEIQLFPKFKAFAKKNGYEIIIAEK
QNWYPDLSFVCEKNPNIKFAVDIKTTYRLDDCLGFCNGFTLGSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASS
GIDETEILQLEKLDNITSVIKDFIFFAEEKWKIASDKGGSGNTANIGSIQCIDDILQGNGMFKNLGEQIFDEYWINQGML
MIPDLKNQGSFKKLTKLADFLEFKGIDIQKINPIKNRSKS

Specific function: Recognizes the double-stranded sequence GATATC and cleaves after T-3 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011337
- InterPro:   IPR011335
- InterPro:   IPR015314
- InterPro:   IPR019755 [H]

Pfam domain/function: PF09233 Endonuc-EcoRV [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 32011; Mature: 32011

Theoretical pI: Translated: 7.86; Mature: 7.86

Prosite motif: PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVKQNFIEKLKVFATELTDHVTTQLGDWKIKGFIDIDKNIYTISSDTKIISKILEIQL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHC
FPKFKAFAKKNGYEIIIAEKQNWYPDLSFVCEKNPNIKFAVDIKTTYRLDDCLGFCNGFT
CHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHCCCC
LGSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASSGIDETEILQLEKLDNITSVI
CCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
KDFIFFAEEKWKIASDKGGSGNTANIGSIQCIDDILQGNGMFKNLGEQIFDEYWINQGML
HHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEE
MIPDLKNQGSFKKLTKLADFLEFKGIDIQKINPIKNRSKS
EECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC
>Mature Secondary Structure
MESVKQNFIEKLKVFATELTDHVTTQLGDWKIKGFIDIDKNIYTISSDTKIISKILEIQL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEECCCHHHHHHHHHHHC
FPKFKAFAKKNGYEIIIAEKQNWYPDLSFVCEKNPNIKFAVDIKTTYRLDDCLGFCNGFT
CHHHHHHHCCCCCEEEEECCCCCCCCCEEEECCCCCEEEEEEEEEEECHHHHHHHHCCCC
LGSHGEYFRNRASTKNIQFPYSHYLAHICLGILYTRSASSGIDETEILQLEKLDNITSVI
CCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHH
KDFIFFAEEKWKIASDKGGSGNTANIGSIQCIDDILQGNGMFKNLGEQIFDEYWINQGML
HHHHHHHHCCEEEECCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEE
MIPDLKNQGSFKKLTKLADFLEFKGIDIQKINPIKNRSKS
EECCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 6328432; 1647200; 8491171; 7819264; 9367757; 9705308; 9545372; 10446231 [H]