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The map label for this gene is mboIIR [H]

Identifier: 188528155

GI number: 188528155

Start: 1372577

End: 1373635

Strand: Reverse

Name: mboIIR [H]

Synonym: HPSH_07070

Alternate gene names: 188528155

Gene position: 1373635-1372577 (Counterclockwise)

Preceding gene: 188528161

Following gene: 188528154

Centisome position: 85.4

GC content: 29.93

Gene sequence:

>1059_bases
ATGAGCAAACGCCCTAGCAATATCATAGGCGAAGAGGTTTATGTAAAAGTGGTTGATAATATCTGCAAGTCTGAAATGCC
TCAAGACAATTTAGGAAAAAAGAATCAAGTAACCCAAGATAGCTTGAGAAAAAATCTTTTTGTGGATATGCATAGAATGG
GGTTGATTGAACGCTACAATAAGAATAAGGAACCTACAAACCCCTACATTCAAAGCAATATTAAATATATCAGTTTAACT
CCCTTAGCTATAGAATTTTTAAACGCGCAAGATTTGTTAAGAAAAAATTTTTGTTACACGCAAGCTTTAGAAAATCTTTT
GCAAGGTTTTGGAGCAGAATGCAGAGAGATAATGATAGAGCTTGACAACCATTATTTAGATATTGAAGAAATGATGTTTT
TTGTTACATTTTTAAATATTGAAAATTTTACTAGAAGTGAAATTATAGAATATGTTAGAGAATATAGGAGTTTAAGCCGT
ATCCAAAAAGAAAAATTAAAAGAGTTAGTGCAAGATTATTGTAACCCTAACCATTTTAACGGAAATAAGCTAGAAAAGAG
AGATTATCATAATTGGAAAAATCAAGCCCAACAAATTTTTAGCTTGCTAGAACAAAGCGTGTTTTTTGAAACCAATAAAG
AGAGGCTTATTTTAAAAACGCTCAATGAAGAAAACAAACAAAACGATAAAAAACTCAAACGCTCTATTAAAGAGAAAGCC
CTTTATTTTGAAAAACATGGCGTTAAAAAAGAAAAGGGCTTTGAATTGCACCATATTGTGCCTTTATGCTTGGCTCGCTC
TATAGAAGAATTTGATCTTTTGGATAAATGGGAAAATTTAATCTATATTGATGCTTTTAACCATGCGAAAATATCTCAAA
CGCAAAATAAACATATTTGTTTGTATTTTAAAGATTGCGATGTGATTTTATCTAAAGGCTTAAAAGAAGAACAAGAAAGC
CTTTATTTTACTTGCATTAAAAATGTGTTATATAAACTTGATTTACAAAATGTCATGTTGAAATACAATAAAGATTTATT
GCATTCTAAAAACGGCTGA

Upstream 100 bases:

>100_bases
TTGTAAAATTTGCTTCTCCTTACCAAATGAGCGCTTCTTTTAATGCTTTAAAATCTTTACTTCTTTATCATTCCCTTTTT
TACAAATTCGCACCACCGAT

Downstream 100 bases:

>100_bases
TAAAATAAGGCGGTGTCTTTTTAAAGAGAGCGTTTTTCATAACCGCTTTTAGTATAAAGGCTATAGAATTAGCGCTACAA
TACCCCCATGCAAAAAAAGA

Product: type IIS restriction enzyme R protein (MBOIIR)

Products: NA

Alternate protein names: R.MboII; Endonuclease MboII; Type II restriction enzyme MboII; Type IIS restriction enzyme MboII [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MSKRPSNIIGEEVYVKVVDNICKSEMPQDNLGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLT
PLAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREIMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSR
IQKEKLKELVQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKTLNEENKQNDKKLKRSIKEKA
LYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKDCDVILSKGLKEEQES
LYFTCIKNVLYKLDLQNVMLKYNKDLLHSKNG

Sequences:

>Translated_352_residues
MSKRPSNIIGEEVYVKVVDNICKSEMPQDNLGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLT
PLAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREIMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSR
IQKEKLKELVQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKTLNEENKQNDKKLKRSIKEKA
LYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKDCDVILSKGLKEEQES
LYFTCIKNVLYKLDLQNVMLKYNKDLLHSKNG
>Mature_351_residues
SKRPSNIIGEEVYVKVVDNICKSEMPQDNLGKKNQVTQDSLRKNLFVDMHRMGLIERYNKNKEPTNPYIQSNIKYISLTP
LAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREIMIELDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRI
QKEKLKELVQDYCNPNHFNGNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKTLNEENKQNDKKLKRSIKEKAL
YFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHICLYFKDCDVILSKGLKEEQESL
YFTCIKNVLYKLDLQNVMLKYNKDLLHSKNG

Specific function: Recognizes the double-stranded sequences GAAGA and TCTTC and cleaves respectively 13 bases after G-1 and 7 bases before T- 1, leaving a single 3' protruding nucleotide [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 41950; Mature: 41819

Theoretical pI: Translated: 8.00; Mature: 8.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRPSNIIGEEVYVKVVDNICKSEMPQDNLGKKNQVTQDSLRKNLFVDMHRMGLIERYN
CCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
KNKEPTNPYIQSNIKYISLTPLAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREIMIE
CCCCCCCCHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRIQKEKLKELVQDYCNPNHFN
HCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKTLNEENKQNDKKLKRSIKEKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHH
LYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHIC
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCCCCEEE
LYFKDCDVILSKGLKEEQESLYFTCIKNVLYKLDLQNVMLKYNKDLLHSKNG
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SKRPSNIIGEEVYVKVVDNICKSEMPQDNLGKKNQVTQDSLRKNLFVDMHRMGLIERYN
CCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHC
KNKEPTNPYIQSNIKYISLTPLAIEFLNAQDLLRKNFCYTQALENLLQGFGAECREIMIE
CCCCCCCCHHHCCCCEEEECHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
LDNHYLDIEEMMFFVTFLNIENFTRSEIIEYVREYRSLSRIQKEKLKELVQDYCNPNHFN
HCCCEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
GNKLEKRDYHNWKNQAQQIFSLLEQSVFFETNKERLILKTLNEENKQNDKKLKRSIKEKA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCCCCHHHHHHHHHHHHHH
LYFEKHGVKKEKGFELHHIVPLCLARSIEEFDLLDKWENLIYIDAFNHAKISQTQNKHIC
HHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCCCCEEE
LYFKDCDVILSKGLKEEQESLYFTCIKNVLYKLDLQNVMLKYNKDLLHSKNG
EEECCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2020540 [H]