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The map label for this gene is phoB [H]

Identifier: 188528154

GI number: 188528154

Start: 1371848

End: 1372489

Strand: Reverse

Name: phoB [H]

Synonym: HPSH_07065

Alternate gene names: 188528154

Gene position: 1372489-1371848 (Counterclockwise)

Preceding gene: 188528155

Following gene: 188528153

Centisome position: 85.32

GC content: 38.16

Gene sequence:

>642_bases
ATGCAAAAAAAGATTTTTTTACTAGAAGACGATTACCTTTTAAGCGAGAGCGTTAAGGAGTTTTTGGAGCATTTAGGCTA
TGAAGTGTCTTGCGCTTTTAACGGGAAAGAAGCTTATGAAAGGCTCTCTGTTGAGCGCTTCAACCTCTTGCTTTTAGATG
TGCAAGTGCCTGAAATGAATAGCTTGGAATTGTTCAGGCGCATCAAAAACGATTTTTTAATCTCCACGCCTGTGATTTTT
ATCACCGCCTTACAGGATAACGCTACCTTAAAAAACGCTTTTAATTTAGGAGCGAGCGATTATTTGAAAAAGCCTTTTGA
TTTGGATGAATTAGAAGCACGCATTAAAAGGTTTTTCAATGATGATCCCATAGAAATCATGCCTAACATTTTTTATCACC
AACACTCTTTGAATATTAAAGGGAAAAAGGAAATCCTAGCGCCCAAAACCGCCCAACTTTTAGAATACTTTTTAGAGCAT
AAGGGGCAAATCATTAGCTCTCAAGCGTTAGAAAATAACTTATGGGAGCAAGCTATTGATGATTCCACCTTGCGCACTTA
CATTAAAGTGTTGCGCAAGCTTTTAGGTAAAAATTGCATAGAAACGCATAAGGGGGTGGGCTATCGCTTTAACCCACTAT
GA

Upstream 100 bases:

>100_bases
TAAAAACGGCTGATAAAATAAGGCGGTGTCTTTTTAAAGAGAGCGTTTTTCATAACCGCTTTTAGTATAAAGGCTATAGA
ATTAGCGCTACAATACCCCC

Downstream 100 bases:

>100_bases
AAAAAAATCCCTTAAACTCTTTTTAGGGACTTATTTGGGCTCTTCGTTTGTGTTGATGCTAGTGATTAGCGTTTTAGCGT
TTAACTATGAAAAAAACGAA

Product: putative transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQKKIFLLEDDYLLSESVKEFLEHLGYEVSCAFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFRRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYHQHSLNIKGKKEILAPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL

Sequences:

>Translated_213_residues
MQKKIFLLEDDYLLSESVKEFLEHLGYEVSCAFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFRRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYHQHSLNIKGKKEILAPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
>Mature_213_residues
MQKKIFLLEDDYLLSESVKEFLEHLGYEVSCAFNGKEAYERLSVERFNLLLLDVQVPEMNSLELFRRIKNDFLISTPVIF
ITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFNDDPIEIMPNIFYHQHSLNIKGKKEILAPKTAQLLEYFLEH
KGQIISSQALENNLWEQAIDDSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL

Specific function: This protein is a positive regulator for the phosphate regulon. Transcription of this operon is positively regulated by phoB and phoR when phosphate is limited [H]

COG id: COG0745

COG function: function code TK; Response regulators consisting of a CheY-like receiver domain and a winged-helix DNA-binding domain

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1789402, Length=218, Percent_Identity=27.0642201834862, Blast_Score=94, Evalue=5e-21,
Organism=Escherichia coli, GI1786784, Length=220, Percent_Identity=26.8181818181818, Blast_Score=88, Evalue=3e-19,
Organism=Escherichia coli, GI1790552, Length=215, Percent_Identity=31.6279069767442, Blast_Score=87, Evalue=7e-19,
Organism=Escherichia coli, GI1786599, Length=226, Percent_Identity=27.4336283185841, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1790860, Length=230, Percent_Identity=28.2608695652174, Blast_Score=83, Evalue=1e-17,
Organism=Escherichia coli, GI1786911, Length=222, Percent_Identity=25.2252252252252, Blast_Score=77, Evalue=9e-16,
Organism=Escherichia coli, GI1787229, Length=225, Percent_Identity=25.3333333333333, Blast_Score=74, Evalue=6e-15,
Organism=Escherichia coli, GI87082012, Length=223, Percent_Identity=26.9058295964126, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1790299, Length=124, Percent_Identity=35.4838709677419, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789809, Length=236, Percent_Identity=26.271186440678, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1790437, Length=113, Percent_Identity=32.7433628318584, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788394, Length=227, Percent_Identity=24.6696035242291, Blast_Score=67, Evalue=8e-13,
Organism=Escherichia coli, GI1788550, Length=114, Percent_Identity=30.7017543859649, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787375, Length=221, Percent_Identity=24.8868778280543, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI2367329, Length=231, Percent_Identity=28.1385281385281, Blast_Score=61, Evalue=7e-11,
Organism=Escherichia coli, GI48994928, Length=120, Percent_Identity=32.5, Blast_Score=60, Evalue=7e-11,
Organism=Saccharomyces cerevisiae, GI6322044, Length=121, Percent_Identity=27.2727272727273, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR011879
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 24834; Mature: 24834

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKKIFLLEDDYLLSESVKEFLEHLGYEVSCAFNGKEAYERLSVERFNLLLLDVQVPEMN
CCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCC
SLELFRRIKNDFLISTPVIFITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFN
HHHHHHHHHHCCEEECCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCC
DDPIEIMPNIFYHQHSLNIKGKKEILAPKTAQLLEYFLEHKGQIISSQALENNLWEQAID
CCCHHHHCHHHHEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
DSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
HHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCC
>Mature Secondary Structure
MQKKIFLLEDDYLLSESVKEFLEHLGYEVSCAFNGKEAYERLSVERFNLLLLDVQVPEMN
CCCEEEEEECCHHHHHHHHHHHHHCCCEEEEEECCHHHHHHHHHHHHEEEEEEEECCCCC
SLELFRRIKNDFLISTPVIFITALQDNATLKNAFNLGASDYLKKPFDLDELEARIKRFFN
HHHHHHHHHHCCEEECCEEEEEEECCCCHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHCC
DDPIEIMPNIFYHQHSLNIKGKKEILAPKTAQLLEYFLEHKGQIISSQALENNLWEQAID
CCCHHHHCHHHHEECCCCCCCCHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCC
DSTLRTYIKVLRKLLGKNCIETHKGVGYRFNPL
HHHHHHHHHHHHHHHCCHHHHHHCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2115874; 10984043 [H]