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The map label for this gene is dnaB [H]

Identifier: 188528151

GI number: 188528151

Start: 1367806

End: 1369272

Strand: Reverse

Name: dnaB [H]

Synonym: HPSH_07050

Alternate gene names: 188528151

Gene position: 1369272-1367806 (Counterclockwise)

Preceding gene: 188528152

Following gene: 188528150

Centisome position: 85.12

GC content: 40.63

Gene sequence:

>1467_bases
ATGGATCATTTAAAGCATTTGCAGCAATTGCAAAATATGGAAAGGATCGTGCTTTCAGGTATTGTGTTGGCCAATCATAA
GATTGAAGAGGTTCATAGCGTTTTAGAGCCTAGCGATTTTTACTACCCGCCTAACGGCTTGTTTTTTGAAATCGCTTTAA
AACTGCATGAAGAAAATTGCCCCATTGATGAGAATTTTATCCGCCAAAAAATGCCTAAAGACAAGCAAATCAAAGAAGAA
GATCTGGTCGCTATTTTTGCGGCAAGCCCTATAGATAATATTGAAGCCTATGTTGAAGAGATTAAAAACGCTTCCATTAA
ACGAAAACTTTTTGGCTTGGCTAACACCATTAGAGAGCAAGCTCTAGAAAGCGCGCAAAAATCCAGCGATATTTTAGGCG
CTGTGGAGCGAGAAGTCTATGCGTTATTGAACGGCAGCACGATAGAGGGCTTTAGGGATATTAAAGAAGTGCTTGAAAGC
ACGATGGATCTTATTGTAGAAAACCAAAGAAGGGGGAGTTTGGAAGTTACTGGCATACCGACTGGCTTTGTCCAGTTGGA
TAATTATACGAGCGGTTTTAATAAGGGGAGTTTAGTCATTATAGGGGCAAGGCCGTCTATGGGTAAAACCAGTTTGATGA
TGAACATGGTCTTAAGCGCGCTCAATGACGATAGGGGGGTAGCGGTTTTTAGTTTAGAAATGTCTGCGGAGCAACTCGCT
TTAAGGGCGTTATCGGATCTCACTTCTATTAACATGCATGATTTAGAAAGCGCAAGGCTTGATGATGATCAATGGGAAAA
TTTAGCCAAATGCTACGATCACCTTTCTTGCAAAAAACTCTTTTTCTACGATAAAAGTCATGTGAGGATAGAGCAAATCC
GCTTGCAATTACGAAAGCTTAAATCCCAACACAAGGAATTGGGTATCGCTTTTATTGACTATTTGCAACTCATGTCAGGG
AGCAAAGCCACTAAAGAACGCCATGAACAGATCGCTGAAATTTCAAGGGAGCTTAAAACTTTAGCCAGAGAATTAGAAAT
CCCTATCATAGCGTTAGTGCAACTCAACCGCAGCCTGGAAAACCGGGAAGATAAACGGCCCATTCTTTCTGATATTAAAG
ATAGCGGAGGGATTGAACAAGACGCTGACATTGTTTTATTTTTGTATAGAGGCTATATCTATCAAATGAGGGCTGAAGAC
AACAAAATAGACAAACTCAAAAAAGAAGGCAAAATTGAAGAGGTGCAAGAGTTGCGCCTAAAAGTCGATGAAGAAAGGCG
TATCCACAAGCAAAATGGCAGTATTGAAGAGGCTGAAATCATTGTGGCCAAAAACAGGAATGGGGCTACAGGAACGGTTT
ATACGCGCTTTAACGCTCCTTTAACGCGCTATGAAGACATGCCTATGGATTCCCATTTAGAAGAAGGACAAGAAACTAAA
GTGGATTTTGATATAGTTACAACTTGA

Upstream 100 bases:

>100_bases
AGTTTAGCGCACGCTATAGCGAGTTTAGAATTTAAGAATAATTACGCTTTAACGCCCCTAGATTTGATAGAAAAGATCAA
ACGATTATAAAAGGATAAAA

Downstream 100 bases:

>100_bases
AAGATAAAACTTTTCAGGGGGCGTTTGAACTTCTTACAACCCCCAAAGAATACTTGCTGTGTGGGGTTATTTTAAGCCTT
TTGTTAGCGCTCAATCTTTA

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 488; Mature: 488

Protein sequence:

>488_residues
MDHLKHLQQLQNMERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEENCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRDIKEVLES
TMDLIVENQRRGSLEVTGIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESARLDDDQWENLAKCYDHLSCKKLFFYDKSHVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENREDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEVQELRLKVDEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPLTRYEDMPMDSHLEEGQETK
VDFDIVTT

Sequences:

>Translated_488_residues
MDHLKHLQQLQNMERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEENCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRDIKEVLES
TMDLIVENQRRGSLEVTGIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESARLDDDQWENLAKCYDHLSCKKLFFYDKSHVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENREDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEVQELRLKVDEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPLTRYEDMPMDSHLEEGQETK
VDFDIVTT
>Mature_488_residues
MDHLKHLQQLQNMERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEENCPIDENFIRQKMPKDKQIKEE
DLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQALESAQKSSDILGAVEREVYALLNGSTIEGFRDIKEVLES
TMDLIVENQRRGSLEVTGIPTGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
LRALSDLTSINMHDLESARLDDDQWENLAKCYDHLSCKKLFFYDKSHVRIEQIRLQLRKLKSQHKELGIAFIDYLQLMSG
SKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLENREDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAED
NKIDKLKKEGKIEEVQELRLKVDEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPLTRYEDMPMDSHLEEGQETK
VDFDIVTT

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=466, Percent_Identity=32.8326180257511, Blast_Score=250, Evalue=2e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 55784; Mature: 55784

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDHLKHLQQLQNMERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEENC
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCEEEEEEEHHCCCC
PIDENFIRQKMPKDKQIKEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQ
CCCHHHHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALESAQKSSDILGAVEREVYALLNGSTIEGFRDIKEVLESTMDLIVENQRRGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
CCEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSDLTSINMHDLESARLDDDQWENLAKCYDHLSCKKLFFYDKSHVRIEQIRLQLRKL
HHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCHHHHEEECCCCCHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NREDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEVQELRL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHEEECCCCCHHHHHHHCCCHHHHHHHHH
KVDEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPLTRYEDMPMDSHLEEGQETK
CCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCHHHHCCCCCHHHHCCCCCCE
VDFDIVTT
ECEEEECC
>Mature Secondary Structure
MDHLKHLQQLQNMERIVLSGIVLANHKIEEVHSVLEPSDFYYPPNGLFFEIALKLHEENC
CCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCEECCCCCEEEEEEEHHCCCC
PIDENFIRQKMPKDKQIKEEDLVAIFAASPIDNIEAYVEEIKNASIKRKLFGLANTIREQ
CCCHHHHHHHCCCCCCCCHHHEEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALESAQKSSDILGAVEREVYALLNGSTIEGFRDIKEVLESTMDLIVENQRRGSLEVTGIP
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECC
TGFVQLDNYTSGFNKGSLVIIGARPSMGKTSLMMNMVLSALNDDRGVAVFSLEMSAEQLA
CCEEEECCCCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHH
LRALSDLTSINMHDLESARLDDDQWENLAKCYDHLSCKKLFFYDKSHVRIEQIRLQLRKL
HHHHHHHHCCCHHHCCCCCCCCHHHHHHHHHHHHHCHHHHEEECCCCCHHHHHHHHHHHH
KSQHKELGIAFIDYLQLMSGSKATKERHEQIAEISRELKTLARELEIPIIALVQLNRSLE
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
NREDKRPILSDIKDSGGIEQDADIVLFLYRGYIYQMRAEDNKIDKLKKEGKIEEVQELRL
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCHHEEECCCCCHHHHHHHCCCHHHHHHHHH
KVDEERRIHKQNGSIEEAEIIVAKNRNGATGTVYTRFNAPLTRYEDMPMDSHLEEGQETK
CCCHHHHHHHCCCCCCEEEEEEEECCCCCCEEEEEEECCCHHHHCCCCCHHHHCCCCCCE
VDFDIVTT
ECEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9252185 [H]