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The map label for this gene is hinfIM [H]

Identifier: 188528141

GI number: 188528141

Start: 1357228

End: 1358307

Strand: Reverse

Name: hinfIM [H]

Synonym: HPSH_07000

Alternate gene names: 188528141

Gene position: 1358307-1357228 (Counterclockwise)

Preceding gene: 188528142

Following gene: 188528140

Centisome position: 84.44

GC content: 32.96

Gene sequence:

>1080_bases
ATGGATTTTTTAAAAGAAAACTTAAATACTATCATAGAGGGGGATTGTTTAGAAAAGTTGAAAGATTTTCCTAACAGAAG
CGTTGATTTTATCTTTGCTGACCCCCCTTATTTCATGCAAACAGAGGGGGAATTGAAGCGTTTTGAAGGCACAAAATTTC
AAGGCGTTGAGGATCATTGGGATAAATTTGGCTCTTTTAAGGAATACGATACCTTTTGTTTGGGTTGGTTAAAAGAATGC
CAAAGGGTTTTAAAAGATAATGGCAGTATTTGTGTGATAGGGAGTTTTCAAAATATTTTTAGAATTGGTTTTCATTTGCA
AAACTTAGGGTTTTGGATACTCAATGATATTATTTGGCACAAGAGTAATCCGGTGCCTAATTTTGCTGGCAAGAGACTAT
GCAACGCCCATGAAACGCTTATTTGGTGCGCTAAACACAAAAACAGCAAAGTTAGTTTTAATTATAAAACAATGAAGTAT
CTTAATAATGACAAACAAGAAAAATCGGTTTGGCAAATCCCTATTTGCATTGGTAACGAAAGGTTAAAAGACGCGCAAGG
CAAGAAAGTGCATTCCACGCAAAAACCAGAAGCGCTTTTAAAAAAAATCATTTTAAGCGCGACTAAGCCTAAAGATATTA
TTTTAGATCCCTTTTTTGGCACAGGCACAACAGGGGCTGTGGCTAAATCCATGAATAGGTATTTTATTGGCATTGAAAAA
GATTCTTTTTATATCAAAGAAGCGGCAAAACGCCTGAATAATACTAGGGATAAAAGCGATTTTATCACTAATTTAGTTTT
AGAAACTAAACCCCCAAAAATCCCTATGAGTCTTTTAATTTCTAAACAATTATTAAAAATTGGAGATTTTTTATACTCAT
CTAACAAAGAAAAAATTTGTCAAGTTTTGGAAAACGGACAAGTGAGGGATAATGAAAATTATGAAACTTCTATCCATAAG
ATGAGCGCTAAATATTTGAATAAAACCAACCATAATGGCTGGAAATTTTTTTATGCGTATTACCAAAATCAATTTTTATT
ACTAGATGAATTGCGTTATATCTGCCAAAAGGAGTTTTAA

Upstream 100 bases:

>100_bases
ATAATTTGATGAGAACAAATCTAGAGATTAAACATTAAGATAGCCTTAAAACGCTTGTGTTAAAATAATTAGAGTAGCAG
ATATAAAAGGTTAGTTAATA

Downstream 100 bases:

>100_bases
TGGATTATCAAACCTTTAACGAGATCTTTAATCGTTTTGTCTTTGGAACATCTAAAGCAAAATTACTTGAAAATATTGCT
GAAAATCCTGAACGCTATTT

Product: adenine specific DNA methyltransferase (HINFIM)

Products: NA

Alternate protein names: M.HinfI; Adenine-specific methyltransferase HinfI [H]

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDHWDKFGSFKEYDTFCLGWLKEC
QRVLKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVSFNYKTMKY
LNNDKQEKSVWQIPICIGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLVLETKPPKIPMSLLISKQLLKIGDFLYSSNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKEF

Sequences:

>Translated_359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDHWDKFGSFKEYDTFCLGWLKEC
QRVLKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVSFNYKTMKY
LNNDKQEKSVWQIPICIGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLVLETKPPKIPMSLLISKQLLKIGDFLYSSNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKEF
>Mature_359_residues
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDHWDKFGSFKEYDTFCLGWLKEC
QRVLKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWHKSNPVPNFAGKRLCNAHETLIWCAKHKNSKVSFNYKTMKY
LNNDKQEKSVWQIPICIGNERLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
DSFYIKEAAKRLNNTRDKSDFITNLVLETKPPKIPMSLLISKQLLKIGDFLYSSNKEKICQVLENGQVRDNENYETSIHK
MSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKEF

Specific function: This methylase recognizes the double-stranded sequence GANTC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the HinfI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=268, Percent_Identity=36.1940298507463, Blast_Score=125, Evalue=4e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 41924; Mature: 41924

Theoretical pI: Translated: 9.23; Mature: 9.23

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDHW
CCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCHHHHCCCCCCCCCHHHH
DKFGSFKEYDTFCLGWLKECQRVLKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWH
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
KSNPVPNFAGKRLCNAHETLIWCAKHKNSKVSFNYKTMKYLNNDKQEKSVWQIPICIGNE
CCCCCCCHHHHHHCCCHHHEEEEECCCCCEEEEEHHHHHHHCCCCCCCCEEEEEEEECCC
RLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEC
DSFYIKEAAKRLNNTRDKSDFITNLVLETKPPKIPMSLLISKQLLKIGDFLYSSNKEKIC
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
QVLENGQVRDNENYETSIHKMSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKEF
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCC
>Mature Secondary Structure
MDFLKENLNTIIEGDCLEKLKDFPNRSVDFIFADPPYFMQTEGELKRFEGTKFQGVEDHW
CCHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCEECCCCHHHHCCCCCCCCCHHHH
DKFGSFKEYDTFCLGWLKECQRVLKDNGSICVIGSFQNIFRIGFHLQNLGFWILNDIIWH
HHCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHC
KSNPVPNFAGKRLCNAHETLIWCAKHKNSKVSFNYKTMKYLNNDKQEKSVWQIPICIGNE
CCCCCCCHHHHHHCCCHHHEEEEECCCCCEEEEEHHHHHHHCCCCCCCCEEEEEEEECCC
RLKDAQGKKVHSTQKPEALLKKIILSATKPKDIILDPFFGTGTTGAVAKSMNRYFIGIEK
HHHCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCCCCCCHHHHHHCCCCEEEEEEC
DSFYIKEAAKRLNNTRDKSDFITNLVLETKPPKIPMSLLISKQLLKIGDFLYSSNKEKIC
CCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHH
QVLENGQVRDNENYETSIHKMSAKYLNKTNHNGWKFFYAYYQNQFLLLDELRYICQKEF
HHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEEHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3063606 [H]