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The map label for this gene is birA [C]

Identifier: 188527923

GI number: 188527923

Start: 1153806

End: 1154444

Strand: Reverse

Name: birA [C]

Synonym: HPSH_05870

Alternate gene names: 188527923

Gene position: 1154444-1153806 (Counterclockwise)

Preceding gene: 188527924

Following gene: 188527922

Centisome position: 71.77

GC content: 38.65

Gene sequence:

>639_bases
ATGAGAAAATGTGAAAAAAGAGTTTTTGATAGCCTGCCTTCCACGCAAACCTATCTTTTAGAAAAACTCAAAAACGATGG
ACTCAAAGCCCCTATTTTGATCGTGGCTAAAAACCAAAGCGCTGGGATAGGCAGTAGGGGGAATATTTGGGAGAGCGCAA
AAAGCGCTTTGACTTTTTCGCTCGCTTTAAACGCAAGCGATTTGCCTAAAGATTTGCCCTTGCAAGCGAGCGCTTTGTAT
TTAGGGTTTTTATTCAAAGAAGTTTTAAAAGATTTAGGCTCTCAAACCTGGCTTAAATGGCCTAACGATTTGTATTTAGG
GGATCAAAAAATAGGGGGAGTGCTGGTTAATGTTTATAAAGACATGCGGGTGTGCGGCATTGGCGTGAATAGGGTTTCAA
AAAAATGGGCATGTTTAGATATTGGCGTAAGCGATGATTTGATTATGGAGGGCTTTTTAAAAAAAATAGAAGAAAATCTT
TTTTGGGGGGAAGTTTTAAGTAAGTATGCGTTAGAATTTCATAGAAGCAACTCTTTTAGCTTCCATAATGATTGGGGTGA
AGCGATGAGTTTGAAAGATGCGGAATTGTTAGAAGACGGTCGCATTTGTATTAAAGGTAAGATTTATGATAGGATGTGA

Upstream 100 bases:

>100_bases
GGAGCGTGCGTATAGCAAGGTTGCAAGCGGTGGGTAAAAAGCCTTTGAAAGCGAAGGATTATTTGAATGGCAAGCGTTTG
AAAGCGGGCGGTATTTTAAC

Downstream 100 bases:

>100_bases
GTATGATGAGTGAAATCATTGCAGTGGCCAATCAAAAAGGGGGTGTGGGCAAAACGACAACGGCGGTTAATTTAGCGGCT
TCTTTAGCAGTGCATGAAAA

Product: biotin--protein ligase

Products: AMP; diphosphate; [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Alternate protein names: Biotin/Acetyl-CoA-Carboxylase Ligase; Biotin-[Acetyl-CoA-Carboxylase] Synthetase; Biotin-[Acetyl-CoA-Carboxylase] Ligase; Biotin Operon Repressor; BirA Bifunctional Protein; Biotin-(Acetyl-CoA-Carboxylase) Ligase; Biotin-Acetyl-CoA-Carboxylase Ligase

Number of amino acids: Translated: 212; Mature: 212

Protein sequence:

>212_residues
MRKCEKRVFDSLPSTQTYLLEKLKNDGLKAPILIVAKNQSAGIGSRGNIWESAKSALTFSLALNASDLPKDLPLQASALY
LGFLFKEVLKDLGSQTWLKWPNDLYLGDQKIGGVLVNVYKDMRVCGIGVNRVSKKWACLDIGVSDDLIMEGFLKKIEENL
FWGEVLSKYALEFHRSNSFSFHNDWGEAMSLKDAELLEDGRICIKGKIYDRM

Sequences:

>Translated_212_residues
MRKCEKRVFDSLPSTQTYLLEKLKNDGLKAPILIVAKNQSAGIGSRGNIWESAKSALTFSLALNASDLPKDLPLQASALY
LGFLFKEVLKDLGSQTWLKWPNDLYLGDQKIGGVLVNVYKDMRVCGIGVNRVSKKWACLDIGVSDDLIMEGFLKKIEENL
FWGEVLSKYALEFHRSNSFSFHNDWGEAMSLKDAELLEDGRICIKGKIYDRM
>Mature_212_residues
MRKCEKRVFDSLPSTQTYLLEKLKNDGLKAPILIVAKNQSAGIGSRGNIWESAKSALTFSLALNASDLPKDLPLQASALY
LGFLFKEVLKDLGSQTWLKWPNDLYLGDQKIGGVLVNVYKDMRVCGIGVNRVSKKWACLDIGVSDDLIMEGFLKKIEENL
FWGEVLSKYALEFHRSNSFSFHNDWGEAMSLKDAELLEDGRICIKGKIYDRM

Specific function: Bira Acts Both As A Biotin-Operon Repressor And As The Enzyme That Synthesizes The Corepressor, Acetyl-CoA:Carbon- Dioxide Ligase. This Protein Also Activates Biotin To Form Biotinyl-5'-Adenylate And Transfers The Biotin Moiety To Biotin-Accepting Protei

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 6.3.4.15

Molecular weight: Translated: 23888; Mature: 23888

Theoretical pI: Translated: 8.30; Mature: 8.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRKCEKRVFDSLPSTQTYLLEKLKNDGLKAPILIVAKNQSAGIGSRGNIWESAKSALTFS
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEE
LALNASDLPKDLPLQASALYLGFLFKEVLKDLGSQTWLKWPNDLYLGDQKIGGVLVNVYK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECCHHHHHHHHHHHH
DMRVCGIGVNRVSKKWACLDIGVSDDLIMEGFLKKIEENLFWGEVLSKYALEFHRSNSFS
HHHHCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
FHNDWGEAMSLKDAELLEDGRICIKGKIYDRM
CCCCHHHHCCCCCHHHHCCCCEEEEEEECCCC
>Mature Secondary Structure
MRKCEKRVFDSLPSTQTYLLEKLKNDGLKAPILIVAKNQSAGIGSRGNIWESAKSALTFS
CCHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHHEEEE
LALNASDLPKDLPLQASALYLGFLFKEVLKDLGSQTWLKWPNDLYLGDQKIGGVLVNVYK
EEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEECCCCCEEECCHHHHHHHHHHHH
DMRVCGIGVNRVSKKWACLDIGVSDDLIMEGFLKKIEENLFWGEVLSKYALEFHRSNSFS
HHHHCCCCHHHHCCCEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
FHNDWGEAMSLKDAELLEDGRICIKGKIYDRM
CCCCHHHHCCCCCHHHHCCCCEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; biotin; apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

Specific reaction: ATP + biotin + apo-[acetyl-CoA:carbon-dioxide ligase (ADP-forming)] = AMP + diphosphate + [acetyl-CoA:carbon-dioxide ligase (ADP-forming)]

General reaction: Amination; Carboxylic acid formation (carboxamide formation [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA