Click here to switch to the map view.

The map label for this gene is trmB [H]

Identifier: 188527778

GI number: 188527778

Start: 997806

End: 998987

Strand: Reverse

Name: trmB [H]

Synonym: HPSH_05045

Alternate gene names: 188527778

Gene position: 998987-997806 (Counterclockwise)

Preceding gene: 188527779

Following gene: 188527777

Centisome position: 62.1

GC content: 38.07

Gene sequence:

>1182_bases
TTGTTTAAGTTTTTCTACCTTTTATTTTTAACTTTGGGGCATCTTCTTGGTGCGCCTTTCATCTTTTTTTGGAGTTTTAA
AGAAAAATACCGCCATTCTTTAAAGGCTCGTTTTTTCCTCAAAGACAATTTTTTAAAAAGCGAGCCGGTTTTTTGGTTTC
ATGCATGCTCTTATGGGGAGGTCAAATCCTTAGAGCCAATCATTCAAGCTTTAAAAGAGCCGATTTTAATCAGCGTTACC
ACTAATACCGGTTTTGAATTAGCCGCTCAAACTTACCAGAATTTGGAGCATATAGAAGTGCGTTACTTGCCTTTTGAAAC
CCTATTATTTGCATGGAAAAAAAACTTAAAACGCTTAAAAACTTTGGTGGTTACAGAAGCGGAATTGTGGTTTAATGTGT
TTGATACGGCTCAAAAATTAGGGGCAAAAACCATGCTCATTAACGCTAGGATCAGCGTTCGTTCTTACCCTAAATACCAG
CGTTTTTCTTTTTTTTATGCGCTTTTATTCAAACGCATTGATTTGGTTTTAGCGCAAAGCAAGGACGATAAAAAGCGCTT
GTTGAATCTAGGGGCAAAAAAAGTGGTGGATTTTTTGAATATCAAGCGTTTTTCAAAGCCTGTAACCGCTTCGTTTTACC
CTAAAAACCCAAGCGCTTTAAACATTGTTTTAGCCAGCACGCATGAGGGCGAGGAGGAATTAGGGTTAAAAGCGTTTTTG
GAGTTCAAAAAGACGCATCAAAACGCAAGGCTTTTTATCGTGCCGCGCCACCCTGAGCGTTTTAAAAGCGTGCAAAATTT
ATTGCAAGATGCTTTAAAAACGACTCGTTTTAGTTGGGAGTGTTTTTCTTCAAAGGGTTTTGTGGAATGCGATATTTTGT
TAGTGGATCGCTTGGGAGAATTGAATAACTTCTATCAAATCGCAGATATTGTCATTTTAGGGGGTTCGTTTGTCAAAATG
GGGGGGCATAACCCTTTAGAGCCGGCGTTTTTTAATGCGCGCTTGATCACAGGGGAGTATATTTTCAACCAAGTAGCGTT
GTTTGAATTAATCAAGCCTTATAAAATCGTTCAAAAAGAGGATTTGTTAGACGCTCTTTTAGATTACAAAAATTTAGGCG
TGGCGCGTTTTTTAGAGAATGGGCATGATTTAAACGAATTGCTCGCATTCATTAAACATTAA

Upstream 100 bases:

>100_bases
TTGCCCGTATTGCGGGCGTATTTTATACGCTGAGGGCGCGTATGAAAACAGCGCTCAACTTCCAAAAGAAAGCCAAGAAG
AAAGCCAAGAATTAGTTTGA

Downstream 100 bases:

>100_bases
AAAGGATAAGGATAAACATGGAAAAAGCTTATAAAATATTGAGCGTTCAAGAAAACATTTCGCACAAAAAAGCCAAAGCT
TTGATTGATTTGGGGTTAGT

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KdtA protein homolog; tRNA (guanine-N(7)-)-methyltransferase; tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQNLEHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLVLAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVTASFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHQNARLFIVPRHPERFKSVQNLLQDALKTTRFSWECFSSKGFVECDILLVDRLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELIKPYKIVQKEDLLDALLDYKNLGVARFLENGHDLNELLAFIKH

Sequences:

>Translated_393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQNLEHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLVLAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVTASFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHQNARLFIVPRHPERFKSVQNLLQDALKTTRFSWECFSSKGFVECDILLVDRLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELIKPYKIVQKEDLLDALLDYKNLGVARFLENGHDLNELLAFIKH
>Mature_393_residues
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGEVKSLEPIIQALKEPILISVT
TNTGFELAAQTYQNLEHIEVRYLPFETLLFAWKKNLKRLKTLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQ
RFSFFYALLFKRIDLVLAQSKDDKKRLLNLGAKKVVDFLNIKRFSKPVTASFYPKNPSALNIVLASTHEGEEELGLKAFL
EFKKTHQNARLFIVPRHPERFKSVQNLLQDALKTTRFSWECFSSKGFVECDILLVDRLGELNNFYQIADIVILGGSFVKM
GGHNPLEPAFFNARLITGEYIFNQVALFELIKPYKIVQKEDLLDALLDYKNLGVARFLENGHDLNELLAFIKH

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Inner Membrane-Anchored [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=346, Percent_Identity=31.7919075144509, Blast_Score=148, Evalue=6e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007507
- InterPro:   IPR003358 [H]

Pfam domain/function: PF04413 Glycos_transf_N; PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 45731; Mature: 45731

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIQALKEPILISVTTNTGFELAAQTYQNLEHIEVRYLPFETLLFAWKKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLVLAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KDDKKRLLNLGAKKVVDFLNIKRFSKPVTASFYPKNPSALNIVLASTHEGEEELGLKAFL
CCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
EFKKTHQNARLFIVPRHPERFKSVQNLLQDALKTTRFSWECFSSKGFVECDILLVDRLGE
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEHHHHH
LNNFYQIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYIFNQVALFELIKPYKIVQKE
HHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
DLLDALLDYKNLGVARFLENGHDLNELLAFIKH
HHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCC
>Mature Secondary Structure
MFKFFYLLFLTLGHLLGAPFIFFWSFKEKYRHSLKARFFLKDNFLKSEPVFWFHACSYGE
CHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHEEEEEECCCCCCCCEEEEEECCCCC
VKSLEPIIQALKEPILISVTTNTGFELAAQTYQNLEHIEVRYLPFETLLFAWKKNLKRLK
CHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHEEEEEECHHHHHHHHHHHHHHHH
TLVVTEAELWFNVFDTAQKLGAKTMLINARISVRSYPKYQRFSFFYALLFKRIDLVLAQS
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHCCC
KDDKKRLLNLGAKKVVDFLNIKRFSKPVTASFYPKNPSALNIVLASTHEGEEELGLKAFL
CCHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCEEEEEEEECCCCHHHHHHHHHH
EFKKTHQNARLFIVPRHPERFKSVQNLLQDALKTTRFSWECFSSKGFVECDILLVDRLGE
HHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCEEEEEEEEHHHHH
LNNFYQIADIVILGGSFVKMGGHNPLEPAFFNARLITGEYIFNQVALFELIKPYKIVQKE
HHHHHHHHHHEEECCCEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHH
DLLDALLDYKNLGVARFLENGHDLNELLAFIKH
HHHHHHHHHHCCCHHHHHHCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11889109 [H]