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The map label for this gene is hypB [H]

Identifier: 188527726

GI number: 188527726

Start: 934215

End: 934943

Strand: Reverse

Name: hypB [H]

Synonym: HPSH_04745

Alternate gene names: 188527726

Gene position: 934943-934215 (Counterclockwise)

Preceding gene: 188527727

Following gene: 188527725

Centisome position: 58.12

GC content: 40.33

Gene sequence:

>729_bases
ATGATCGAACAACGAAAAGAATCTTTACAAAATAACCCTAATTTGAGTAAAAAAGATGTCAAAATCGTGGAAAAGATTTT
GAGCAAGAACGACATTAAAGCCGCTGAAATGAAAGAACGCTACCTGAAAGAAGGGCTGTATGTGTTGAATTTCATGAGCT
CTCCAGGCAGCGGTAAAACCACGATGCTAGAAAACCTAGCGGATTTTAAAGACTTTAAGTTTTGCGTGGTAGAGGGCGAT
TTGCAAACCAATAGAGATGCGGACAGATTGCGTAAAAAAGGCGTGAGCGCGCACCAGATCACCACCGGCGAAGCGTGCCA
TTTGGAAGCGAGCATGATTGAAGGGGCGTTTGATTTGCTCAAAGATGAGGGAGCGTTAGAAAAAAGCGATTTTTTAATCA
TTGAAAATGTAGGGAATCTGGTTTGCCCTTCAAGCTATAATCTGGGAGCGGCGATGAATATCGTTTTACTCTCAGTCCCA
GAGGGCGATGATAAGGTGCTAAAATACCCTACGATGTTCATGTGCGCTGATGCGGTGATTATCAGTAAAGCGGATATGAT
TGAAGTGTTTAATTTTAGGGTTTCTCAAGTCAAAGAAGACATGCAAAAATTAAAGCCTGAAGCGCCTATTTTTTTAATGA
GCTCCAAAGACCCTAAAAGCTTGGAAGATTTTAAAAATTTCCTTTTAGAAAAAAAGCGTGAAAATTACCAATCCACGCAT
TCGTTTTAA

Upstream 100 bases:

>100_bases
GAGCTAAAAATCAGCCAAAAAGATAAAAAAAGCCGCTTAAATCATAGCCGTTTAATTAAGCGCGCTAGGATACGAAAATT
TTAAAAGAAAGGAACAAGCA

Downstream 100 bases:

>100_bases
TGTGTTTAGCGATCCCTTCTAAAGTCATAGCCATTAACGATAATGTGGCGCTCTTAGAAACTTTAGGCGTTCAAAGAGAG
GCGAGCTTGGATTTAATGGG

Product: hydrogenase expression/formation protein HypB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 242; Mature: 242

Protein sequence:

>242_residues
MIEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKTTMLENLADFKDFKFCVVEGD
LQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLLKDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVP
EGDDKVLKYPTMFMCADAVIISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTH
SF

Sequences:

>Translated_242_residues
MIEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKTTMLENLADFKDFKFCVVEGD
LQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLLKDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVP
EGDDKVLKYPTMFMCADAVIISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTH
SF
>Mature_242_residues
MIEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKTTMLENLADFKDFKFCVVEGD
LQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLLKDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVP
EGDDKVLKYPTMFMCADAVIISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTH
SF

Specific function: Plays a role in both the hydrogenase and the urease nickel cofactor insertion step [H]

COG id: COG0378

COG function: function code OK; Ni2+-binding GTPase involved in regulation of expression and maturation of urease and hydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the hypB/hupM family [H]

Homologues:

Organism=Escherichia coli, GI1789082, Length=225, Percent_Identity=40.8888888888889, Blast_Score=160, Evalue=5e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003495
- InterPro:   IPR004392
- InterPro:   IPR012202 [H]

Pfam domain/function: PF02492 cobW [H]

EC number: NA

Molecular weight: Translated: 27351; Mature: 27351

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKT
CCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHH
TMLENLADFKDFKFCVVEGDLQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLL
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHH
KDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVPEGDDKVLKYPTMFMCADAVI
HCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHH
ISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
SF
CC
>Mature Secondary Structure
MIEQRKESLQNNPNLSKKDVKIVEKILSKNDIKAAEMKERYLKEGLYVLNFMSSPGSGKT
CCCHHHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEECCCCCCHH
TMLENLADFKDFKFCVVEGDLQTNRDADRLRKKGVSAHQITTGEACHLEASMIEGAFDLL
HHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCCEEECCCCCHHHHHHHHHHHHHHH
KDEGALEKSDFLIIENVGNLVCPSSYNLGAAMNIVLLSVPEGDDKVLKYPTMFMCADAVI
HCCCCCCCCCEEEEECCCCEECCCCCCCCCEEEEEEEECCCCCCCEEECCHHHHHHHHHH
ISKADMIEVFNFRVSQVKEDMQKLKPEAPIFLMSSKDPKSLEDFKNFLLEKKRENYQSTH
HHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHC
SF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682; 11123699 [H]