Click here to switch to the map view.

The map label for this gene is topA [H]

Identifier: 188527700

GI number: 188527700

Start: 906132

End: 908066

Strand: Reverse

Name: topA [H]

Synonym: HPSH_04610

Alternate gene names: 188527700

Gene position: 908066-906132 (Counterclockwise)

Preceding gene: 188527701

Following gene: 188527699

Centisome position: 56.45

GC content: 30.9

Gene sequence:

>1935_bases
ATGTATAAAAACTGCGTATTTATCATAGAAAGTCCCAATAAGATCGCTAAAATCAAAGAATTAACAGGAAGTTCATTTGT
TTTTGCGACTGGTGGGCATTTTGTTGAGCTAGTCAATATTGAAGTGAGTAAAGAATTTAATCCTATTTTTGAAATCAAAA
AAAGCACGGATAAGAAAAAGGATAAATCCACTCATATCAACTATATGATAAATCAATGTAAAGATAAAGTGGTCTATATT
GCTACTGACCCTGATAGAGAGGGTTATGGTATAGGTTATAAATTTTATGAAAAAATCAAAAATATAGCTAAAACAATTTA
TCGCACTGAATTTCATGAAATCACAAAAAGCGGTGTAGAAAAAGGCTTGAACAATGCTGTGCTATTTTCTCAAAGCAATC
TCAATCTATATTATAGTTGGCTAGGTAGGATTGTAAGCGATCAATTTGTAGGCTTTACTTTAACGCCTTATTTGCGCAAA
AACATTAAAAATTTTGAAGTGAGTGCTGGTAGGGTTCAAACTACTGCTCTTTCCATTTTAGTAGAATTAGACAGAAAAAT
CCAAGCCTTTGAACAAAAGAATATTGATGAGAAATTAAGTTATAGCATAGAAGCTATTATTGATGTGTTAGGTTCTCAAA
TCTCTATTACTTTAGTAGAAGAGAATAAAAGGAAAGTGTTTGAGACCAAAGAATTAGCCCAAAACTTTTTAAATGACTTA
AAAAACAATCTTAACCCCTTAGCTTTTTTAGACGCAGTAGAGCAAAAAGATAAAGAAAAATCTCCACCTAAACCTTTCAC
CACTTCTAATCTTTTAAAAGATGGGGTTAGAATTTTAGAAATGGGCGTTAAGCAAATCCAAGAACACGCCCAAAAACTTT
TTGAAGCTGGATTGATTACTTATATTCGCACCGATAGTGAAGCGTTAAGTAAAGAATACTTGCAAGAGCATCAAGCGTTT
TTTGAAAATATTTATCCTAGCGTGTATGAGTACAGAGAATATAGGGCTGGTAAAAATTCACAAGCTGAAGCGCATGAGGC
TATAAGAATTACACACCCTCACTGCTATGAAGATCTTAAAAAAGTGTGCGAGAAACACAATATTACAGATATTAATGATT
TAAAAGTCTATACGCTTATCTTTTTTAACACCATTTGCTCTCAAAGTAAAAACGCTATTTACGAAAACACAACTTTAAAT
TTTAAAGTAAAAACGCACAGCTTTAAATGCAGTTTTAGCAAACTCAAATCTAAAGGCTTTAAAGCGATTAAAGACTTAGA
AGAAGAAAAGAAAGATGAAGAAGAGATTGAAAGCGATCTTGATTTTTCTAGCTTGCAATTAAAAACTCAAATGCCTATTT
TAGATTTTAATATCAAAGAACTAAAAACTAAAGCTCCTAGCCCTTATACTGAAAGCACTTTTATCGCAATGATGGAAACC
TATGGCATAGGAAGACCTAGCACTTATACAAGTGTATTTGAGATCTTAAAAAACAAAAATTACATTACATTAGAGGGCAA
AAATAGGAAGATCACGCCCACAGCTCTTGGTAAAAGCATTGTTGATTTCTTTTTGAATGATAACCAAACACAATGGATTG
CTATTTCTAAAGTGGATAATAGTTTTACAAAAAAATTAGAAGAAATGTTAGATATGATTATAAAAGATGGTAAAAGTGCT
TATCTTGATTTAATGCAAAATATCCAAAAGAAATTAGGCACAGAAATTTCTAACTTATATAGAAATAATAGCAATAACAA
CATTTCTGCTACAAAAAAAGAAATGATACCTCCAACTGAAAAACAACTTAATTTTGTAGAAACTATAGAAAAAACTCTTC
AAATAAAAGCTTCTGATATGACGAAAAAAGACAAGTTTGCGTGCATGAAGTTTATAGAAGAACATAGTAAAAAAATGCCC
AAAAAGGATAATTGA

Upstream 100 bases:

>100_bases
AATAGAAAAATCCTCAATGATCTTAAAAAATCAAACCAAGAAACTTATAAGGAAGAGTATTTAAAAACTAAAATGAAAAA
AGAAAGCGAGAATATTCAAT

Downstream 100 bases:

>100_bases
TGGTATTAAAACTTGTATCTATTTGCTATGCAGTCATTATTCTTATGGGTTGCTCCAATAAACAATATGAAATGTATAAA
AGCCCTTGCGCGAACTTAGA

Product: topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 644; Mature: 644

Protein sequence:

>644_residues
MYKNCVFIIESPNKIAKIKELTGSSFVFATGGHFVELVNIEVSKEFNPIFEIKKSTDKKKDKSTHINYMINQCKDKVVYI
ATDPDREGYGIGYKFYEKIKNIAKTIYRTEFHEITKSGVEKGLNNAVLFSQSNLNLYYSWLGRIVSDQFVGFTLTPYLRK
NIKNFEVSAGRVQTTALSILVELDRKIQAFEQKNIDEKLSYSIEAIIDVLGSQISITLVEENKRKVFETKELAQNFLNDL
KNNLNPLAFLDAVEQKDKEKSPPKPFTTSNLLKDGVRILEMGVKQIQEHAQKLFEAGLITYIRTDSEALSKEYLQEHQAF
FENIYPSVYEYREYRAGKNSQAEAHEAIRITHPHCYEDLKKVCEKHNITDINDLKVYTLIFFNTICSQSKNAIYENTTLN
FKVKTHSFKCSFSKLKSKGFKAIKDLEEEKKDEEEIESDLDFSSLQLKTQMPILDFNIKELKTKAPSPYTESTFIAMMET
YGIGRPSTYTSVFEILKNKNYITLEGKNRKITPTALGKSIVDFFLNDNQTQWIAISKVDNSFTKKLEEMLDMIIKDGKSA
YLDLMQNIQKKLGTEISNLYRNNSNNNISATKKEMIPPTEKQLNFVETIEKTLQIKASDMTKKDKFACMKFIEEHSKKMP
KKDN

Sequences:

>Translated_644_residues
MYKNCVFIIESPNKIAKIKELTGSSFVFATGGHFVELVNIEVSKEFNPIFEIKKSTDKKKDKSTHINYMINQCKDKVVYI
ATDPDREGYGIGYKFYEKIKNIAKTIYRTEFHEITKSGVEKGLNNAVLFSQSNLNLYYSWLGRIVSDQFVGFTLTPYLRK
NIKNFEVSAGRVQTTALSILVELDRKIQAFEQKNIDEKLSYSIEAIIDVLGSQISITLVEENKRKVFETKELAQNFLNDL
KNNLNPLAFLDAVEQKDKEKSPPKPFTTSNLLKDGVRILEMGVKQIQEHAQKLFEAGLITYIRTDSEALSKEYLQEHQAF
FENIYPSVYEYREYRAGKNSQAEAHEAIRITHPHCYEDLKKVCEKHNITDINDLKVYTLIFFNTICSQSKNAIYENTTLN
FKVKTHSFKCSFSKLKSKGFKAIKDLEEEKKDEEEIESDLDFSSLQLKTQMPILDFNIKELKTKAPSPYTESTFIAMMET
YGIGRPSTYTSVFEILKNKNYITLEGKNRKITPTALGKSIVDFFLNDNQTQWIAISKVDNSFTKKLEEMLDMIIKDGKSA
YLDLMQNIQKKLGTEISNLYRNNSNNNISATKKEMIPPTEKQLNFVETIEKTLQIKASDMTKKDKFACMKFIEEHSKKMP
KKDN
>Mature_644_residues
MYKNCVFIIESPNKIAKIKELTGSSFVFATGGHFVELVNIEVSKEFNPIFEIKKSTDKKKDKSTHINYMINQCKDKVVYI
ATDPDREGYGIGYKFYEKIKNIAKTIYRTEFHEITKSGVEKGLNNAVLFSQSNLNLYYSWLGRIVSDQFVGFTLTPYLRK
NIKNFEVSAGRVQTTALSILVELDRKIQAFEQKNIDEKLSYSIEAIIDVLGSQISITLVEENKRKVFETKELAQNFLNDL
KNNLNPLAFLDAVEQKDKEKSPPKPFTTSNLLKDGVRILEMGVKQIQEHAQKLFEAGLITYIRTDSEALSKEYLQEHQAF
FENIYPSVYEYREYRAGKNSQAEAHEAIRITHPHCYEDLKKVCEKHNITDINDLKVYTLIFFNTICSQSKNAIYENTTLN
FKVKTHSFKCSFSKLKSKGFKAIKDLEEEKKDEEEIESDLDFSSLQLKTQMPILDFNIKELKTKAPSPYTESTFIAMMET
YGIGRPSTYTSVFEILKNKNYITLEGKNRKITPTALGKSIVDFFLNDNQTQWIAISKVDNSFTKKLEEMLDMIIKDGKSA
YLDLMQNIQKKLGTEISNLYRNNSNNNISATKKEMIPPTEKQLNFVETIEKTLQIKASDMTKKDKFACMKFIEEHSKKMP
KKDN

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI4507635, Length=506, Percent_Identity=22.5296442687747, Blast_Score=102, Evalue=1e-21,
Organism=Escherichia coli, GI1787529, Length=585, Percent_Identity=27.008547008547, Blast_Score=171, Evalue=1e-43,
Organism=Escherichia coli, GI1788061, Length=479, Percent_Identity=22.1294363256785, Blast_Score=77, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17555378, Length=482, Percent_Identity=25.103734439834, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24585251, Length=475, Percent_Identity=23.3684210526316, Blast_Score=96, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013826
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 74299; Mature: 74299

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKNCVFIIESPNKIAKIKELTGSSFVFATGGHFVELVNIEVSKEFNPIFEIKKSTDKKK
CCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHCCCCC
DKSTHINYMINQCKDKVVYIATDPDREGYGIGYKFYEKIKNIAKTIYRTEFHEITKSGVE
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLNNAVLFSQSNLNLYYSWLGRIVSDQFVGFTLTPYLRKNIKNFEVSAGRVQTTALSIL
HCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECHHHHCCCCCCCCCCCHHHHHHHHHH
VELDRKIQAFEQKNIDEKLSYSIEAIIDVLGSQISITLVEENKRKVFETKELAQNFLNDL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
KNNLNPLAFLDAVEQKDKEKSPPKPFTTSNLLKDGVRILEMGVKQIQEHAQKLFEAGLIT
HHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
YIRTDSEALSKEYLQEHQAFFENIYPSVYEYREYRAGKNSQAEAHEAIRITHPHCYEDLK
EEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHEEECCCHHHHHHH
KVCEKHNITDINDLKVYTLIFFNTICSQSKNAIYENTTLNFKVKTHSFKCSFSKLKSKGF
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEEEECEEECCHHHHHHHHH
KAIKDLEEEKKDEEEIESDLDFSSLQLKTQMPILDFNIKELKTKAPSPYTESTFIAMMET
HHHHHHHHHCCCHHHHHHCCCHHHEEEEECCCEECCCHHHHHCCCCCCCCCHHHHHHHHH
YGIGRPSTYTSVFEILKNKNYITLEGKNRKITPTALGKSIVDFFLNDNQTQWIAISKVDN
HCCCCCCHHHHHHHHHHCCCEEEEECCCCEECHHHHHHHHHHHHHCCCCEEEEEEEECCC
SFTKKLEEMLDMIIKDGKSAYLDLMQNIQKKLGTEISNLYRNNSNNNISATKKEMIPPTE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHCCCCCH
KQLNFVETIEKTLQIKASDMTKKDKFACMKFIEEHSKKMPKKDN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYKNCVFIIESPNKIAKIKELTGSSFVFATGGHFVELVNIEVSKEFNPIFEIKKSTDKKK
CCCCEEEEEECCCHHHHHHHCCCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHCCCCC
DKSTHINYMINQCKDKVVYIATDPDREGYGIGYKFYEKIKNIAKTIYRTEFHEITKSGVE
CHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KGLNNAVLFSQSNLNLYYSWLGRIVSDQFVGFTLTPYLRKNIKNFEVSAGRVQTTALSIL
HCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEECHHHHCCCCCCCCCCCHHHHHHHHHH
VELDRKIQAFEQKNIDEKLSYSIEAIIDVLGSQISITLVEENKRKVFETKELAQNFLNDL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHHHHH
KNNLNPLAFLDAVEQKDKEKSPPKPFTTSNLLKDGVRILEMGVKQIQEHAQKLFEAGLIT
HHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHE
YIRTDSEALSKEYLQEHQAFFENIYPSVYEYREYRAGKNSQAEAHEAIRITHPHCYEDLK
EEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCHHHHHEEECCCHHHHHHH
KVCEKHNITDINDLKVYTLIFFNTICSQSKNAIYENTTLNFKVKTHSFKCSFSKLKSKGF
HHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEEEECEEECCHHHHHHHHH
KAIKDLEEEKKDEEEIESDLDFSSLQLKTQMPILDFNIKELKTKAPSPYTESTFIAMMET
HHHHHHHHHCCCHHHHHHCCCHHHEEEEECCCEECCCHHHHHCCCCCCCCCHHHHHHHHH
YGIGRPSTYTSVFEILKNKNYITLEGKNRKITPTALGKSIVDFFLNDNQTQWIAISKVDN
HCCCCCCHHHHHHHHHHCCCEEEEECCCCEECHHHHHHHHHHHHHCCCCEEEEEEEECCC
SFTKKLEEMLDMIIKDGKSAYLDLMQNIQKKLGTEISNLYRNNSNNNISATKKEMIPPTE
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCHHHCCCCCH
KQLNFVETIEKTLQIKASDMTKKDKFACMKFIEEHSKKMPKKDN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]