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The map label for this gene is dapF

Identifier: 188527587

GI number: 188527587

Start: 789668

End: 790489

Strand: Direct

Name: dapF

Synonym: HPSH_04045

Alternate gene names: 188527587

Gene position: 789668-790489 (Clockwise)

Preceding gene: 188527585

Following gene: 188527588

Centisome position: 49.09

GC content: 39.05

Gene sequence:

>822_bases
ATGGTGTTTTACAAATATTCAGGGAGCGGGAATGATTTTTTAATCGTTCAAAGCTTCAAAAAAAAAGATTTTTCAAATTT
AGCCAAACAGGTGTGCCATAGGCATGAGGGTTTTGGGGCTGATGGGCTTGTAGTCGTCTTACCGAGTAAGGATTATGACT
ACGAATGGGATTTTTACAATTCAGACGGCTCTAAAGCTGGCATGTGCGGGAATGCGAGCCGTTGTGTGGGGTTATTCGCC
TACCAACATGCTATAGCCTCTAAAAACCATGTTTTTTTAGCCGGAAAAAGAGAGATTTCTATTTGCATAGAAGAGCCCAA
TATTATAGAGAGCAATCTTGGTAACTACAAAATCCTAGACACTATACCCGCTTTAAGATGCGAAAAGTTTTTTACCAACG
ACAGCGTTTTAGAAAATATCCCTACTTTCTACCTCATAGATACAGGAGTGCCGCATTTAGTGGGATTTGTGAAAAATAAA
GAGTGGTTAAATTCCCTTAACACGCTGGAATTAAGGGCTTTAAGGCATGCGTTTAACGCTAATATTAACATCGCTTTTAT
AGAAAATAAAGAGACGATTTTTTTACAAACTTATGAAAGAGGGGTTGAAGATTTCACGCTAGCTTGCGGGACGGGCATGG
CGGCGGTTTTTATCGCTGCACGCATTTTTTATAACACGCCTGAAAAAGCCGCTCTCATCCCTAAAAGCAGTGAATCTTTA
GAACTTTCTTTAAAAAATGATGAAATTTTTTATAAAGGCGCGGTGCGTTATATTGGCATGAGTGTTTTGGGCATGAGTGT
TTTTGAAAATGGGTGTTTTTGA

Upstream 100 bases:

>100_bases
TAAACTGAACCAAAAATTATCAAATGTTAGCTAAAAAACGCTCTTTTAAGGATAAAAAACCTCTTTTAACTTATAATTAA
AACCAAAAAGGCTTATATAA

Downstream 100 bases:

>100_bases
TCGTTATTTCTTGTAATAGTAATGATTTTGTTGCAATTAAACATAATGCGCTATCATTTGCGAGTTGTTTTTAGCAATAT
AAAAAATATCCTAAAGGAAT

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 273; Mature: 273

Protein sequence:

>273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDTIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EWLNSLNTLELRALRHAFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPEKAALIPKSSESL
ELSLKNDEIFYKGAVRYIGMSVLGMSVFENGCF

Sequences:

>Translated_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDTIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EWLNSLNTLELRALRHAFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPEKAALIPKSSESL
ELSLKNDEIFYKGAVRYIGMSVLGMSVFENGCF
>Mature_273_residues
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYNSDGSKAGMCGNASRCVGLFA
YQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILDTIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNK
EWLNSLNTLELRALRHAFNANINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPEKAALIPKSSESL
ELSLKNDEIFYKGAVRYIGMSVLGMSVFENGCF

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=225, Percent_Identity=28, Blast_Score=85, Evalue=5e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30625; Mature: 30625

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHCCCCCEEEEE
TIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNKEWLNSLNTLELRALRHAFNA
CCCHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPEKAALIPKSSESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCCE
ELSLKNDEIFYKGAVRYIGMSVLGMSVFENGCF
EEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MVFYKYSGSGNDFLIVQSFKKKDFSNLAKQVCHRHEGFGADGLVVVLPSKDYDYEWDFYN
CEEEEEECCCCEEEEEECHHCCCHHHHHHHHHHHCCCCCCCCEEEEECCCCCCEECEEEC
SDGSKAGMCGNASRCVGLFAYQHAIASKNHVFLAGKREISICIEEPNIIESNLGNYKILD
CCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECCCCHHCCCCCEEEEE
TIPALRCEKFFTNDSVLENIPTFYLIDTGVPHLVGFVKNKEWLNSLNTLELRALRHAFNA
CCCHHHHHHHCCCCHHHHHCCEEEEEECCCHHHHHHHCCHHHHHCCCHHHHHHHHHHHCC
NINIAFIENKETIFLQTYERGVEDFTLACGTGMAAVFIAARIFYNTPEKAALIPKSSESL
CEEEEEEECCCEEEEEEHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEECCCCCCCE
ELSLKNDEIFYKGAVRYIGMSVLGMSVFENGCF
EEEECCCEEEEEHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA