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The map label for this gene is nth [H]

Identifier: 188527568

GI number: 188527568

Start: 772662

End: 773318

Strand: Direct

Name: nth [H]

Synonym: HPSH_03950

Alternate gene names: 188527568

Gene position: 772662-773318 (Clockwise)

Preceding gene: 188527567

Following gene: 188527569

Centisome position: 48.03

GC content: 41.25

Gene sequence:

>657_bases
GTGAAAATGGGTTTAAAACGCGCTAAAACTTACCAAAAAGCCCAACAAATCAAAAAACTGCTTTTAAAACATTACCCCAA
CCAAACCACCGAATTGCACCATAAAAACCCCTATGAATTGCTAGTGGCTACCATTTTAAGCGCTCAATGCACGGACGCTA
GAGTGAATAAAATAACGCCCAAGTTATTTGAAAAATACCCAAGCGTGAAGGATTTAGCGCTCGCTTCTTTAGAAGAAGTT
AAAGAAACCATCAAATCCGTTTCTTATTTCAACAATAAAAGCAAGCATTTAATCAGTATGGCGCAAAAAGTGGTTAGGGA
TTTTAAGGGCGTTATCCCCTCTACGCAAAAAGAATTGATGAGCCTAGATGGCGTGGGGCAAAAAACCGCTAATGTGGTGC
TTTCAGTGTGCTTTGATGCAAATTATATAGCCGTAGATACCCATGTGTTCCGCACGACTCACCGCTTAGGCTTAAGCAAC
GCTAACACGCCCATTAAAACCGAAGAAGAACTCAGCGATCTTTTTAAAGACAACCTATCCAAACTCCACCATGCCTTAAT
CTTGTTTGGCCGCTACACCTGCAAGGCTAAAAACCCCTTATGCGGCGCGTGTTTTTTAAAAGAATTTTGCGTTTCTAAGG
CTAGCTTTAAAGCGTAG

Upstream 100 bases:

>100_bases
ATTATTCCATGAAAAAAGCCACGCTTTCGGTTAAAATCAACCGCATTCAAGTGGCTTTAAGATCCCATGAAGCGCAATAC
CTTCTCATCAAAGAAAGCGT

Downstream 100 bases:

>100_bases
TGGCTTTATTTGTTATAAAATTTATAGTAAAATTAAGATTTCATAATCAAAGTTAAATCATTCTTGAAAGAGTAGGGTTT
TATGTCAGAAACAGAATCTA

Product: endonuclease III (nth)

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 218; Mature: 218

Protein sequence:

>218_residues
MKMGLKRAKTYQKAQQIKKLLLKHYPNQTTELHHKNPYELLVATILSAQCTDARVNKITPKLFEKYPSVKDLALASLEEV
KETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVVLSVCFDANYIAVDTHVFRTTHRLGLSN
ANTPIKTEEELSDLFKDNLSKLHHALILFGRYTCKAKNPLCGACFLKEFCVSKASFKA

Sequences:

>Translated_218_residues
MKMGLKRAKTYQKAQQIKKLLLKHYPNQTTELHHKNPYELLVATILSAQCTDARVNKITPKLFEKYPSVKDLALASLEEV
KETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVVLSVCFDANYIAVDTHVFRTTHRLGLSN
ANTPIKTEEELSDLFKDNLSKLHHALILFGRYTCKAKNPLCGACFLKEFCVSKASFKA
>Mature_218_residues
MKMGLKRAKTYQKAQQIKKLLLKHYPNQTTELHHKNPYELLVATILSAQCTDARVNKITPKLFEKYPSVKDLALASLEEV
KETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELMSLDGVGQKTANVVLSVCFDANYIAVDTHVFRTTHRLGLSN
ANTPIKTEEELSDLFKDNLSKLHHALILFGRYTCKAKNPLCGACFLKEFCVSKASFKA

Specific function: Has both an apurinic and/or apyrimidinic endonuclease activity and a DNA N-glycosylase activity. Incises damaged DNA at cytosines, thymines and guanines. Acts on a damaged strand, 5' from the damaged site [H]

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI115298648, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI115298650, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI115298654, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI115298652, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI6912520, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=5e-15,
Organism=Homo sapiens, GI190358497, Length=188, Percent_Identity=27.1276595744681, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI4505471, Length=177, Percent_Identity=27.683615819209, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1787920, Length=202, Percent_Identity=38.1188118811881, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI1789331, Length=187, Percent_Identity=26.2032085561497, Blast_Score=75, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554540, Length=180, Percent_Identity=31.1111111111111, Blast_Score=93, Evalue=1e-19,
Organism=Drosophila melanogaster, GI45550361, Length=186, Percent_Identity=30.1075268817204, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24578; Mature: 24578

Theoretical pI: Translated: 10.10; Mature: 10.10

Prosite motif: PS00764 ENDONUCLEASE_III_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMGLKRAKTYQKAQQIKKLLLKHYPNQTTELHHKNPYELLVATILSAQCTDARVNKITP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCH
KLFEKYPSVKDLALASLEEVKETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
SLDGVGQKTANVVLSVCFDANYIAVDTHVFRTTHRLGLSNANTPIKTEEELSDLFKDNLS
HHCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KLHHALILFGRYTCKAKNPLCGACFLKEFCVSKASFKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKMGLKRAKTYQKAQQIKKLLLKHYPNQTTELHHKNPYELLVATILSAQCTDARVNKITP
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHCCH
KLFEKYPSVKDLALASLEEVKETIKSVSYFNNKSKHLISMAQKVVRDFKGVIPSTQKELM
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHH
SLDGVGQKTANVVLSVCFDANYIAVDTHVFRTTHRLGLSNANTPIKTEEELSDLFKDNLS
HHCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHH
KLHHALILFGRYTCKAKNPLCGACFLKEFCVSKASFKA
HHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]