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The map label for this gene is rpoN [H]

Identifier: 188527441

GI number: 188527441

Start: 622709

End: 623953

Strand: Direct

Name: rpoN [H]

Synonym: HPSH_03295

Alternate gene names: 188527441

Gene position: 622709-623953 (Clockwise)

Preceding gene: 188527440

Following gene: 188527442

Centisome position: 38.71

GC content: 36.79

Gene sequence:

>1245_bases
ATGGCGGTTTTACGCGCAAACCTTAGCCCTAAAAACAAATTAAACGCTACCTTAAAAGGGTGGCTTCCCATTTTACAAAG
CGAGCTTGAAGATTTAGAAGAAGTGTTGAAACAAAACGCCCTAGATAACCCCTTAATCAAAATTGAAAACAAGCGCATCA
AAAATTTTAGCGATCGTTTTAGCGCTAAAAATAGCAGCGATCATTTAGAAAATTTCGCAATCGCTTCTAAAAGCCTTTTT
GAAACCTTAGAAGCTCAAATCATCCCCCCCCTCTTTCCCACTGAAACCTCTCAAAAAATCGCTATGGATATTATCAATGG
GCTAGATAGTGAAGGGTATTTTGAAGAAAACATTGAAGAAAGGGCTAAAATTTTAGGGGTAGAGAGCGAAGTTTATGAAA
AAGTGCGCCAGCGTTTTAGTTACCTTAATCCCGCTGGCATTGGTGCTAGAGATGTGAAAGAGAGCTTTTTATTCCAGCTA
GAGGGTAGGGAATTAGACAATAATGAGCTTTATGAAGAAGCGCGAAAAATCATTTTAAATTTAGAAAAACACCATGAATT
TTCTAAAGATTTTTATTATGAAAAGGCTTTAAAGATTTTAAGATCCTTTAAAAACCCCCCAGCCATTGAGTTTTTAGAAA
AAGAAATAGAAGTCATTCCGGAGCTTTTTATTCTAGAAGTGGATGGTGAAATCATCGTGCGTTTGAATGATGAGAGCTAC
CCAACGATCAGTTTAGAAGAAAATCGCTTTAAAGATAGCGCTTATTTAAAAGAAAAATTAAAAGAGGCTAAGGATTTGAT
TGATGCGTTGAGTTTGAGAAAAGCCACGATTTATAAAATCGGCCTCATGCTTTTAGAGTATCAATACGATTTTTTTAAGG
GTAAGGAATTGCGCCCTTTAAAATTGTTGGATTTAGCCAATGAGTTTAACCACTCTGTAAGCACGATTTCAAGGGCCATT
TCTAATAAATATTTGGCATGCCAAAGGGGGGTTTTCCCTATTAAACATTTCTTTAGCACCGCCTTAGACAATAGCGAGAC
TTCAAACGCTGTGATTAAAGACTACCTTTTAGAATTGATCAAAAACGAAGACAAAAAAGAGCCTTTGAGCGACGCTAAGA
TTTTAGAACTCATTGAAGAAAAATTCCATTTGAAAATGGTAAGAAGAACGATCACCAAATACCGCCAACTGCTCAACATC
GCTTCTTCAAGCGAAAGGAAAAAGCTCTATTTGATGCGCGCTTGA

Upstream 100 bases:

>100_bases
TTAAAAGCGGCACGCTTTTAGCGAGCGGGAACGCTAATGAAATTTATGAAAACGCTCTGGTGCGTAAGTATTATTTAGGG
GAAAATTTTAAGGTATAGAC

Downstream 100 bases:

>100_bases
AAATAACCAGTCAAGTTCTACTCAAATGGATCAGAAAAAATCCGCTTAAGCTATCTTGACCCTAACAAACATATAAAAAG
AATGATTTTAGAGAGGTGAG

Product: RNA polymerase factor sigma-54

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 414; Mature: 413

Protein sequence:

>414_residues
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKNFSDRFSAKNSSDHLENFAIASKSLF
ETLEAQIIPPLFPTETSQKIAMDIINGLDSEGYFEENIEERAKILGVESEVYEKVRQRFSYLNPAGIGARDVKESFLFQL
EGRELDNNELYEEARKIILNLEKHHEFSKDFYYEKALKILRSFKNPPAIEFLEKEIEVIPELFILEVDGEIIVRLNDESY
PTISLEENRFKDSAYLKEKLKEAKDLIDALSLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAI
SNKYLACQRGVFPIKHFFSTALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNI
ASSSERKKLYLMRA

Sequences:

>Translated_414_residues
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKNFSDRFSAKNSSDHLENFAIASKSLF
ETLEAQIIPPLFPTETSQKIAMDIINGLDSEGYFEENIEERAKILGVESEVYEKVRQRFSYLNPAGIGARDVKESFLFQL
EGRELDNNELYEEARKIILNLEKHHEFSKDFYYEKALKILRSFKNPPAIEFLEKEIEVIPELFILEVDGEIIVRLNDESY
PTISLEENRFKDSAYLKEKLKEAKDLIDALSLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAI
SNKYLACQRGVFPIKHFFSTALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNI
ASSSERKKLYLMRA
>Mature_413_residues
AVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKNFSDRFSAKNSSDHLENFAIASKSLFE
TLEAQIIPPLFPTETSQKIAMDIINGLDSEGYFEENIEERAKILGVESEVYEKVRQRFSYLNPAGIGARDVKESFLFQLE
GRELDNNELYEEARKIILNLEKHHEFSKDFYYEKALKILRSFKNPPAIEFLEKEIEVIPELFILEVDGEIIVRLNDESYP
TISLEENRFKDSAYLKEKLKEAKDLIDALSLRKATIYKIGLMLLEYQYDFFKGKELRPLKLLDLANEFNHSVSTISRAIS
NKYLACQRGVFPIKHFFSTALDNSETSNAVIKDYLLELIKNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIA
SSSERKKLYLMRA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1508

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma54 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-54 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789594, Length=346, Percent_Identity=30.3468208092486, Blast_Score=152, Evalue=3e-38,

Paralogues:

None

Copy number: 70 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000394
- InterPro:   IPR007046
- InterPro:   IPR007634 [H]

Pfam domain/function: PF00309 Sigma54_AID; PF04963 Sigma54_CBD; PF04552 Sigma54_DBD [H]

EC number: NA

Molecular weight: Translated: 48100; Mature: 47969

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS00717 SIGMA54_1 ; PS00718 SIGMA54_2 ; PS50044 SIGMA54_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKNFSDRF
CCEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCHHHHH
SAKNSSDHLENFAIASKSLFETLEAQIIPPLFPTETSQKIAMDIINGLDSEGYFEENIEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
RAKILGVESEVYEKVRQRFSYLNPAGIGARDVKESFLFQLEGRELDNNELYEEARKIILN
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LEKHHEFSKDFYYEKALKILRSFKNPPAIEFLEKEIEVIPELFILEVDGEIIVRLNDESY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCC
PTISLEENRFKDSAYLKEKLKEAKDLIDALSLRKATIYKIGLMLLEYQYDFFKGKELRPL
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KLLDLANEFNHSVSTISRAISNKYLACQRGVFPIKHFFSTALDNSETSNAVIKDYLLELI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIASSSERKKLYLMRA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC
>Mature Secondary Structure 
AVLRANLSPKNKLNATLKGWLPILQSELEDLEEVLKQNALDNPLIKIENKRIKNFSDRF
CEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCHHHHCHHHHH
SAKNSSDHLENFAIASKSLFETLEAQIIPPLFPTETSQKIAMDIINGLDSEGYFEENIEE
CCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCHHHHHHH
RAKILGVESEVYEKVRQRFSYLNPAGIGARDVKESFLFQLEGRELDNNELYEEARKIILN
HHHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
LEKHHEFSKDFYYEKALKILRSFKNPPAIEFLEKEIEVIPELFILEVDGEIIVRLNDESY
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCEEEEEECCEEEEEECCCCC
PTISLEENRFKDSAYLKEKLKEAKDLIDALSLRKATIYKIGLMLLEYQYDFFKGKELRPL
CEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
KLLDLANEFNHSVSTISRAISNKYLACQRGVFPIKHFFSTALDNSETSNAVIKDYLLELI
HHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHH
KNEDKKEPLSDAKILELIEEKFHLKMVRRTITKYRQLLNIASSSERKKLYLMRA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]