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The map label for this gene is rimO [H]

Identifier: 188527423

GI number: 188527423

Start: 604162

End: 605481

Strand: Direct

Name: rimO [H]

Synonym: HPSH_03170

Alternate gene names: 188527423

Gene position: 604162-605481 (Clockwise)

Preceding gene: 188527422

Following gene: 188527424

Centisome position: 37.56

GC content: 40.98

Gene sequence:

>1320_bases
ATGCAAATTAAAGAAAACAAACAACTCTGCTTAATTTCATTAGGTTGCTCTAAAAATTTGGTGGATTCAGAAGTGATGTT
AGGCAAGCTTTATAACTACACGCTCACTAATGACACTAAGAGCGCTGATGTGATTTTGATCAACACTTGCGGTTTTATTG
AAAGCGCCAAACAAGAGAGCATCCAAACCATTCTAAACGCCGCTAAAGACAAAAAAAAAGGGGCGATTTTGATTGCGAGC
GGGTGCTTGAGCGAGCGCTATAAAGATGAAATCAAAGAATTGATCCCTGAAGTGGATATTTTTACCGGCGTGGGGGATTA
TGACAAGATTGATATAATGATTGCTAAAAAACAAAACCAGTTCAGCGAGCAAGTGTTTTTAAGCGAGCATTATAACGCGC
GCATCATCACGGGATCGAGCGTGCATGCGTATGTTAAAATTTCTGAGGGTTGCAATCAAAAATGCTCTTTTTGCGCTATC
CCTAGCTTTAAGGGGAAATTACAAAGCAGGGAATTAGACTCCATTTTAAAAGAAGCGGAAAATCTCGCACTTAAAGGTTA
TACGGATATGACTTTTATCGCTCAAGACTCTAGCTCCTTTTTATACGATAAGGGGCAAAAAGACGGCTTGATCCAGCTCA
TTAGTGCGATTGACAAGCAGCAAGCCTTAAAGAGCGCGCGCATCTTATACCTCTACCCTTCTAGCACCACGCTAGAGCTT
ATTAGCACGATTGAAAGTTCGCCCATTTTTCAAAATTATTTTGACATGCCCATCCAGCATATCAGCGACTCCATGCTTAA
AAAAATGCGGCGCAACTCCAGCCAAGCGCACCATTTAAAGCTTTTAGATGCCATGAAGCAGGTTAAAGAAAGCTTTATAA
GAAGCACGATCATTGTAGGGCATCCGGAAGAAAATGAGGGCGAATTTGAGGAATTGAGCGCGTTTTTAGACGAGTTCCGG
TTTGACCGGTTGAATATCTTTGCTTTCAGCGCTGAAGAAAACACGCATGCCTATTCTTTAGAGAAAGTGCCTAAAAAAAT
CATCAACGCTCGCATTAAAGCCTTGAATAAAATCGCTCTAAAGCACCAGAACAATTCCTTTAAGGCTTTGTTAAATAAGC
CCATTAAGGCGTTAGTGGAAAATAAAGAGGGCGAGTATTTTTACAAAGCAAGGGATTTGAGATGGGCGCCTGAAGTGGAT
GGGGAAATCTTAATCAATGACAGCGAACTAGCCACCCCTTTAAAACCCGGGCATTATACGATTATGCCTAGCGCATTTAA
AGACAATATCTTACTCGCTAAGGTTTTAAGCCCTTTTTAA

Upstream 100 bases:

>100_bases
ACAAGCGCGAAATACAAAGCCGATGCGTTTTTAAAAGACGCTCCTGAATGGATTGATTTCTTTTGGGAAGTGGATTTGAA
AAACTTAAAAAGCCATTAAC

Downstream 100 bases:

>100_bases
AAGTTAGGGCTAAAAGCGTGGCTAGAGCGTGGGGGTTTGCGTTTTTTTTTAAATGCGTCAGAAAAATCCGGCAAGAGGCA
AAAATGAAAAACATTTATTC

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO [H]

Number of amino acids: Translated: 439; Mature: 439

Protein sequence:

>439_residues
MQIKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDTKSADVILINTCGFIESAKQESIQTILNAAKDKKKGAILIAS
GCLSERYKDEIKELIPEVDIFTGVGDYDKIDIMIAKKQNQFSEQVFLSEHYNARIITGSSVHAYVKISEGCNQKCSFCAI
PSFKGKLQSRELDSILKEAENLALKGYTDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALKSARILYLYPSSTTLEL
ISTIESSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLDAMKQVKESFIRSTIIVGHPEENEGEFEELSAFLDEFR
FDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQNNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVD
GEILINDSELATPLKPGHYTIMPSAFKDNILLAKVLSPF

Sequences:

>Translated_439_residues
MQIKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDTKSADVILINTCGFIESAKQESIQTILNAAKDKKKGAILIAS
GCLSERYKDEIKELIPEVDIFTGVGDYDKIDIMIAKKQNQFSEQVFLSEHYNARIITGSSVHAYVKISEGCNQKCSFCAI
PSFKGKLQSRELDSILKEAENLALKGYTDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALKSARILYLYPSSTTLEL
ISTIESSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLDAMKQVKESFIRSTIIVGHPEENEGEFEELSAFLDEFR
FDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQNNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVD
GEILINDSELATPLKPGHYTIMPSAFKDNILLAKVLSPF
>Mature_439_residues
MQIKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDTKSADVILINTCGFIESAKQESIQTILNAAKDKKKGAILIAS
GCLSERYKDEIKELIPEVDIFTGVGDYDKIDIMIAKKQNQFSEQVFLSEHYNARIITGSSVHAYVKISEGCNQKCSFCAI
PSFKGKLQSRELDSILKEAENLALKGYTDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALKSARILYLYPSSTTLEL
ISTIESSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLDAMKQVKESFIRSTIIVGHPEENEGEFEELSAFLDEFR
FDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIALKHQNNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVD
GEILINDSELATPLKPGHYTIMPSAFKDNILLAKVLSPF

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12 [H]

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 MTTase N-terminal domain [H]

Homologues:

Organism=Homo sapiens, GI93277076, Length=369, Percent_Identity=24.390243902439, Blast_Score=115, Evalue=8e-26,
Organism=Homo sapiens, GI28872782, Length=401, Percent_Identity=25.4364089775561, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI28872784, Length=401, Percent_Identity=25.4364089775561, Blast_Score=111, Evalue=2e-24,
Organism=Escherichia coli, GI1787057, Length=430, Percent_Identity=31.3953488372093, Blast_Score=206, Evalue=2e-54,
Organism=Escherichia coli, GI1786882, Length=394, Percent_Identity=26.3959390862944, Blast_Score=127, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17553146, Length=455, Percent_Identity=22.6373626373626, Blast_Score=119, Evalue=4e-27,
Organism=Drosophila melanogaster, GI21356207, Length=441, Percent_Identity=24.0362811791383, Blast_Score=117, Evalue=1e-26,
Organism=Drosophila melanogaster, GI19922432, Length=368, Percent_Identity=23.3695652173913, Blast_Score=103, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840 [H]

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004 [H]

EC number: NA

Molecular weight: Translated: 49600; Mature: 49600

Theoretical pI: Translated: 7.23; Mature: 7.23

Prosite motif: PS01278 UPF0004

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDTKSADVILINTCGFIESAKQES
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHHH
IQTILNAAKDKKKGAILIASGCLSERYKDEIKELIPEVDIFTGVGDYDKIDIMIAKKQNQ
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCC
FSEQVFLSEHYNARIITGSSVHAYVKISEGCNQKCSFCAIPSFKGKLQSRELDSILKEAE
HHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCEEECCCHHCHHHHHHHHHHHHHHH
NLALKGYTDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALKSARILYLYPSSTTLEL
CCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHH
ISTIESSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLDAMKQVKESFIRSTIIVG
HHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEE
HPEENEGEFEELSAFLDEFRFDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIAL
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KHQNNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDGEILINDSELATPLKPGHYT
HCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEE
IMPSAFKDNILLAKVLSPF
EECCCCCCCEEHHHHCCCC
>Mature Secondary Structure
MQIKENKQLCLISLGCSKNLVDSEVMLGKLYNYTLTNDTKSADVILINTCGFIESAKQES
CCCCCCCCEEEEEECCCCCCCHHHHHHHHHEEEEECCCCCCCCEEEEECCCCHHHHHHHH
IQTILNAAKDKKKGAILIASGCLSERYKDEIKELIPEVDIFTGVGDYDKIDIMIAKKQNQ
HHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEECCCCCCCEEEEEEECCCCC
FSEQVFLSEHYNARIITGSSVHAYVKISEGCNQKCSFCAIPSFKGKLQSRELDSILKEAE
HHHHHHHHCCCCEEEEECCCEEEEEEECCCCCCCCCEEECCCHHCHHHHHHHHHHHHHHH
NLALKGYTDMTFIAQDSSSFLYDKGQKDGLIQLISAIDKQQALKSARILYLYPSSTTLEL
CCEEECCCCEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHHHHHCEEEEEECCCCHHHH
ISTIESSPIFQNYFDMPIQHISDSMLKKMRRNSSQAHHLKLLDAMKQVKESFIRSTIIVG
HHHHCCCCCHHHHHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCEEEEE
HPEENEGEFEELSAFLDEFRFDRLNIFAFSAEENTHAYSLEKVPKKIINARIKALNKIAL
CCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KHQNNSFKALLNKPIKALVENKEGEYFYKARDLRWAPEVDGEILINDSELATPLKPGHYT
HCCCCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEE
IMPSAFKDNILLAKVLSPF
EECCCCCCCEEHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA