Click here to switch to the map view.

The map label for this gene is prs [H]

Identifier: 188527414

GI number: 188527414

Start: 597129

End: 598085

Strand: Reverse

Name: prs [H]

Synonym: HPSH_03125

Alternate gene names: 188527414

Gene position: 598085-597129 (Counterclockwise)

Preceding gene: 188527415

Following gene: 188527412

Centisome position: 37.18

GC content: 43.78

Gene sequence:

>957_bases
ATGAAGGCGCGTGGGTTTAAGACAAAGATGCGTGGGTTTAAGATTTTTTCAGGGAGCGCTCACCCTGCATTTGGCAAAGA
AGTGTCAAAGCATTTAGGCTTTCCCTTATCCAAAGCGGTGATAGGCAAATTCAGCGATGGCGAAATCAATATCCAAATCA
GCGAATCGGTGCGCGGTAAGGATATTTTTATTATTCAGCCCACTTGCGTGCCGGTCAATGACAATTTAATGGAATTGTTA
GTCATGGTAGATGCTTTAAGGCGCAGTTCAGCCAATTCTATCACAGCGGTGTTGCCGTATTTTGGCTATGCCAGACAGGA
CAGAAAAGCGGCTCCAAGAGTGCCTATCACGGCTAAAATGGTCGCTAATTTGATGCAAGAAGTGGGGATTGAAAGGATCA
TTACGATGGATTTGCATGCCGGGCAAATCCAAGGCTTTTTTGATGTGCCGGTGGATAATTTATACGGATCTATCGTCTTT
AGAGATTATATCCGCTCTAAAGCGTTAAAAAACCCTGTGATCGCTAGCCCTGATGTGGGTGGGGTTACAAGAGCCAGGTA
TTTTGCTAATCAAATGGGCTTAGATTTAATCATCGTGGATAAGCGCCGTGAAAAAGCTAATGAAAGCGAAGTGATGAATA
TTATCGGCTCAGCAAAGGAGCGCGATGTGATTTTAGTGGATGACATGATTGATACCGCAGGCACGATCTGTAAAGCCGCT
TTAGCTTTAAAAGAACAAGGGGCAACTTCTGTCATGGCGTTAGGCACGCATGCGGTTTTGAGTGGGAATGCGATCAAGCG
CATTAAAGAAAGCGCGTTAGATGAAGTGGTGGTAACTAACTCTATCCCTTTAGTTCAAAAATGCGATAAAATCACCACTT
TAAGCGTAGCGCCCTTATTTGCAGAAGTGATCAGAAGGATTTATCATAACGAAAGCGTTCAATCGCTTTTCACTTAA

Upstream 100 bases:

>100_bases
ACCTTAAAAAACTTATTAGAAAAGGAGCTTGGTTGAAAACAAACGCTTTTAGTTTGGGTGTGCTACAATTGATTTTAATT
CATTTTAGGGAGTGTAAGCG

Downstream 100 bases:

>100_bases
AAAGAGAGCAGTTAAAAAGGGGGCAGTTAAAAAGGGAGAGATCAAAAAGGGGGAGTGGTGGATTCTGTAGGACTTGAACC
TACGACCAATCGGTTATGAG

Product: ribose-phosphate pyrophosphokinase

Products: NA

Alternate protein names: RPPK; Phosphoribosyl pyrophosphate synthase; P-Rib-PP synthase; PRPP synthase [H]

Number of amino acids: Translated: 318; Mature: 318

Protein sequence:

>318_residues
MKARGFKTKMRGFKIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGKDIFIIQPTCVPVNDNLMELL
VMVDALRRSSANSITAVLPYFGYARQDRKAAPRVPITAKMVANLMQEVGIERIITMDLHAGQIQGFFDVPVDNLYGSIVF
RDYIRSKALKNPVIASPDVGGVTRARYFANQMGLDLIIVDKRREKANESEVMNIIGSAKERDVILVDDMIDTAGTICKAA
LALKEQGATSVMALGTHAVLSGNAIKRIKESALDEVVVTNSIPLVQKCDKITTLSVAPLFAEVIRRIYHNESVQSLFT

Sequences:

>Translated_318_residues
MKARGFKTKMRGFKIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGKDIFIIQPTCVPVNDNLMELL
VMVDALRRSSANSITAVLPYFGYARQDRKAAPRVPITAKMVANLMQEVGIERIITMDLHAGQIQGFFDVPVDNLYGSIVF
RDYIRSKALKNPVIASPDVGGVTRARYFANQMGLDLIIVDKRREKANESEVMNIIGSAKERDVILVDDMIDTAGTICKAA
LALKEQGATSVMALGTHAVLSGNAIKRIKESALDEVVVTNSIPLVQKCDKITTLSVAPLFAEVIRRIYHNESVQSLFT
>Mature_318_residues
MKARGFKTKMRGFKIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGKDIFIIQPTCVPVNDNLMELL
VMVDALRRSSANSITAVLPYFGYARQDRKAAPRVPITAKMVANLMQEVGIERIITMDLHAGQIQGFFDVPVDNLYGSIVF
RDYIRSKALKNPVIASPDVGGVTRARYFANQMGLDLIIVDKRREKANESEVMNIIGSAKERDVILVDDMIDTAGTICKAA
LALKEQGATSVMALGTHAVLSGNAIKRIKESALDEVVVTNSIPLVQKCDKITTLSVAPLFAEVIRRIYHNESVQSLFT

Specific function: Utilized by both the de novo and the salvage pathways by which endogenously formed or exogenously added pyrimidine, purine, or pyridine bases are converted to the corresponding ribonucleoside monophosphates. [C]

COG id: COG0462

COG function: function code FE; Phosphoribosylpyrophosphate synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribose-phosphate pyrophosphokinase family [H]

Homologues:

Organism=Homo sapiens, GI4506127, Length=314, Percent_Identity=48.0891719745223, Blast_Score=303, Evalue=1e-82,
Organism=Homo sapiens, GI4506129, Length=314, Percent_Identity=48.0891719745223, Blast_Score=301, Evalue=5e-82,
Organism=Homo sapiens, GI28557709, Length=314, Percent_Identity=47.4522292993631, Blast_Score=297, Evalue=8e-81,
Organism=Homo sapiens, GI84875539, Length=317, Percent_Identity=47.6340694006309, Blast_Score=296, Evalue=1e-80,
Organism=Homo sapiens, GI4506133, Length=355, Percent_Identity=36.3380281690141, Blast_Score=194, Evalue=1e-49,
Organism=Homo sapiens, GI194018537, Length=344, Percent_Identity=36.6279069767442, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI310128524, Length=141, Percent_Identity=35.4609929078014, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310115209, Length=141, Percent_Identity=35.4609929078014, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310118259, Length=141, Percent_Identity=35.4609929078014, Blast_Score=93, Evalue=3e-19,
Organism=Homo sapiens, GI310119946, Length=141, Percent_Identity=35.4609929078014, Blast_Score=93, Evalue=3e-19,
Organism=Escherichia coli, GI1787458, Length=313, Percent_Identity=53.9936102236422, Blast_Score=349, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17554702, Length=315, Percent_Identity=46.031746031746, Blast_Score=293, Evalue=9e-80,
Organism=Caenorhabditis elegans, GI25149168, Length=314, Percent_Identity=46.1783439490446, Blast_Score=293, Evalue=1e-79,
Organism=Caenorhabditis elegans, GI71989924, Length=315, Percent_Identity=46.031746031746, Blast_Score=292, Evalue=2e-79,
Organism=Caenorhabditis elegans, GI17554704, Length=309, Percent_Identity=46.2783171521036, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI17570245, Length=338, Percent_Identity=33.4319526627219, Blast_Score=194, Evalue=6e-50,
Organism=Saccharomyces cerevisiae, GI6319403, Length=313, Percent_Identity=43.1309904153355, Blast_Score=277, Evalue=1e-75,
Organism=Saccharomyces cerevisiae, GI6320946, Length=309, Percent_Identity=42.3948220064725, Blast_Score=273, Evalue=2e-74,
Organism=Saccharomyces cerevisiae, GI6321776, Length=311, Percent_Identity=44.0514469453376, Blast_Score=262, Evalue=5e-71,
Organism=Saccharomyces cerevisiae, GI6322667, Length=200, Percent_Identity=41, Blast_Score=162, Evalue=7e-41,
Organism=Saccharomyces cerevisiae, GI6324511, Length=110, Percent_Identity=43.6363636363636, Blast_Score=101, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355239, Length=316, Percent_Identity=46.5189873417722, Blast_Score=294, Evalue=5e-80,
Organism=Drosophila melanogaster, GI45551540, Length=339, Percent_Identity=43.3628318584071, Blast_Score=281, Evalue=4e-76,
Organism=Drosophila melanogaster, GI24651458, Length=351, Percent_Identity=35.6125356125356, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI24651456, Length=351, Percent_Identity=35.6125356125356, Blast_Score=202, Evalue=2e-52,
Organism=Drosophila melanogaster, GI281362873, Length=351, Percent_Identity=35.6125356125356, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651454, Length=351, Percent_Identity=35.6125356125356, Blast_Score=202, Evalue=3e-52,
Organism=Drosophila melanogaster, GI24651462, Length=370, Percent_Identity=34.8648648648649, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24651464, Length=370, Percent_Identity=34.8648648648649, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI45552010, Length=370, Percent_Identity=34.8648648648649, Blast_Score=193, Evalue=1e-49,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000842
- InterPro:   IPR005946
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.7.6.1 [H]

Molecular weight: Translated: 34855; Mature: 34855

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00114 PRPP_SYNTHETASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKARGFKTKMRGFKIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGK
CCCCCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCC
DIFIIQPTCVPVNDNLMELLVMVDALRRSSANSITAVLPYFGYARQDRKAAPRVPITAKM
EEEEECCEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCCCCCCCCCHHHH
VANLMQEVGIERIITMDLHAGQIQGFFDVPVDNLYGSIVFRDYIRSKALKNPVIASPDVG
HHHHHHHCCHHEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GVTRARYFANQMGLDLIIVDKRREKANESEVMNIIGSAKERDVILVDDMIDTAGTICKAA
CHHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHH
LALKEQGATSVMALGTHAVLSGNAIKRIKESALDEVVVTNSIPLVQKCDKITTLSVAPLF
HHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHEEECCCCHHHHHCCCHHEEHHHHHH
AEVIRRIYHNESVQSLFT
HHHHHHHHCCHHHHHHCC
>Mature Secondary Structure
MKARGFKTKMRGFKIFSGSAHPAFGKEVSKHLGFPLSKAVIGKFSDGEINIQISESVRGK
CCCCCCHHHHCCEEEECCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCEEEEEECCCCCCC
DIFIIQPTCVPVNDNLMELLVMVDALRRSSANSITAVLPYFGYARQDRKAAPRVPITAKM
EEEEECCEEECCCHHHHHHHHHHHHHHHCCCCCCEEHHHHHCCCCCCCCCCCCCCCHHHH
VANLMQEVGIERIITMDLHAGQIQGFFDVPVDNLYGSIVFRDYIRSKALKNPVIASPDVG
HHHHHHHCCHHEEEEEECCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
GVTRARYFANQMGLDLIIVDKRREKANESEVMNIIGSAKERDVILVDDMIDTAGTICKAA
CHHHHHHHHHHCCCEEEEEECCHHCCCHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHH
LALKEQGATSVMALGTHAVLSGNAIKRIKESALDEVVVTNSIPLVQKCDKITTLSVAPLF
HHHHHCCCCEEEECCCHHHHCCHHHHHHHHHHHHHEEECCCCHHHHHCCCHHEEHHHHHH
AEVIRRIYHNESVQSLFT
HHHHHHHHCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]