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The map label for this gene is prrB [H]

Identifier: 188527306

GI number: 188527306

Start: 495914

End: 497182

Strand: Direct

Name: prrB [H]

Synonym: HPSH_02565

Alternate gene names: 188527306

Gene position: 495914-497182 (Clockwise)

Preceding gene: 188527305

Following gene: 188527307

Centisome position: 30.83

GC content: 34.59

Gene sequence:

>1269_bases
ATGCATAAAATAGAGCGCTTACTCCAAACTTTAGTGCCTAAGGGGGTGGAGTTTAAAACGCTTGAAGAGGTTTTTGAAAT
TAAAAATGGTTACACCCCATCAAAAAACAATCCTGAATTTTGGGAAAAAGGGACTATCCCTTGGTTTAGAATGGAAGACA
TTAGAGAAAATGGGAGGATTTTAAAAGACTCTATCCAACACATTACCCCAAAGGCTTTAAAGGGTAAGAAATTATTCCCT
AAAAATTCTATTATTATTTCTACAACGGCAACGATAGGAGAGCATGCCCTTTTAATCGTTGATTCGTTAGCGAATCAACA
ATTCACTTTTTTAAGCAAAAAAGCGAATTGTGATATTGCTTTAGACATGAAATTCTTTTTTTATCAATGTTTTCTTTTGG
GGGAATGGTGCAAAAAAAACACTAATGTTTCAGGTTTTGCTTCTATGGATATGACTGCTTTTAAAAAATATAAGTTCCCC
ATCCCACCCCTAGAAATCCAACAAGAGATCGTTAAGATTTTGGACGCTTTCACAGAATTAAACACAGAATTAAACACAGA
ATTAAACACAGAATTAAAAGCGCGAAAAAAGCAATACCAGTATTACCAAAACATGCTTTTAGACTTTAAGGACATCCATT
CAAACCATAAAGACGCAAAAATTAGCGCAAAAACCTACCCCAAACGCTTAAAAACCTTACTCCAAACCTTAGCGCCTAAG
GGGGTGGAGTTTAGGAAATTGGGGGAGGTGTGTGAAATAATTAGGGGTAAAAGGGTTACAAAAAAAGAAATATTAGATAA
AGGAAAATATCCCGTTGTATCTGGTGGAATAGGATTTATGGGATATTTAAATGAATATAACAGAGAGGAAAATACAATCA
CTATAGCTCAATATGGAACTGCCGGATTTGTCAATTGGCAAAATCAAAAGTTTTGGGCAAATGATGTTTGTTTTTCTGTC
ATTCCAAAAGAAACCCTAATCAATAGATACCTTTATTATGTATTGACAAACATGCAAAATTATTTATATTCTATTTCAAA
TAGAAGCGCTATACCTTATAGCATTTCTAGTAACAACATTATGCAAATAACAATCCCCATCCCACCCCTAGAAATCCAAC
AAGAGATCGTTAAGATTTTGGATCAATTCTTGGCCCTAACCACCGATTTATTAGCCGGTATCCCGGCTGAAATAGAAGCG
AGAAAAAAGCAATACGAATATTACAGAGAAAAACTACTGACTTTTAAACCACTCCAAAACAAGGCATAA

Upstream 100 bases:

>100_bases
ATTAAAGCGCTCAATGGTGAGATTTCTCAAATCGTAGAAAAACAAAGCGCTTTAAGGAACAGCCTTGAATCAATCATCAA
AGAGTTAGAAGAGGGGCAAA

Downstream 100 bases:

>100_bases
ACCATGAGTTATGAGATTATCGCAGAAAGCAATGAAAGCACCGTGATGGCTGAGTATCATAGCGATAATGAAAAAAAAAG
CGCTTATGAGAGCGAAGCAG

Product: anti-codon nuclease masking agent (prrB)

Products: NA

Alternate protein names: S.EcoprrI; Type I restriction enzyme EcoprrI specificity protein; S protein [H]

Number of amino acids: Translated: 422; Mature: 422

Protein sequence:

>422_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPK
GVEFRKLGEVCEIIRGKRVTKKEILDKGKYPVVSGGIGFMGYLNEYNREENTITIAQYGTAGFVNWQNQKFWANDVCFSV
IPKETLINRYLYYVLTNMQNYLYSISNRSAIPYSISSNNIMQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEA
RKKQYEYYREKLLTFKPLQNKA

Sequences:

>Translated_422_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPK
GVEFRKLGEVCEIIRGKRVTKKEILDKGKYPVVSGGIGFMGYLNEYNREENTITIAQYGTAGFVNWQNQKFWANDVCFSV
IPKETLINRYLYYVLTNMQNYLYSISNRSAIPYSISSNNIMQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEA
RKKQYEYYREKLLTFKPLQNKA
>Mature_422_residues
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRILKDSIQHITPKALKGKKLFP
KNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIALDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFP
IPPLEIQQEIVKILDAFTELNTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPK
GVEFRKLGEVCEIIRGKRVTKKEILDKGKYPVVSGGIGFMGYLNEYNREENTITIAQYGTAGFVNWQNQKFWANDVCFSV
IPKETLINRYLYYVLTNMQNYLYSISNRSAIPYSISSNNIMQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEA
RKKQYEYYREKLLTFKPLQNKA

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 48964; Mature: 48964

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCH
LKDSIQHITPKALKGKKLFPKNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEHHCCCCCCEE
LDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCC
GVEFRKLGEVCEIIRGKRVTKKEILDKGKYPVVSGGIGFMGYLNEYNREENTITIAQYGT
CCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHCCCCCCEEEEEECCC
AGFVNWQNQKFWANDVCFSVIPKETLINRYLYYVLTNMQNYLYSISNRSAIPYSISSNNI
CEEEECCCCCEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCE
MQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARKKQYEYYREKLLTFKPLQN
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KA
CC
>Mature Secondary Structure
MHKIERLLQTLVPKGVEFKTLEEVFEIKNGYTPSKNNPEFWEKGTIPWFRMEDIRENGRI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCEEEHHHHHHCCCH
LKDSIQHITPKALKGKKLFPKNSIIISTTATIGEHALLIVDSLANQQFTFLSKKANCDIA
HHHHHHHCCHHHHCCCCCCCCCCEEEEECCCCCCCCEEEEEHHCCCCEEEHHCCCCCCEE
LDMKFFFYQCFLLGEWCKKNTNVSGFASMDMTAFKKYKFPIPPLEIQQEIVKILDAFTEL
EHHHHHHHHHHHHHHHHHCCCCCCCEECCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
NTELNTELNTELKARKKQYQYYQNMLLDFKDIHSNHKDAKISAKTYPKRLKTLLQTLAPK
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHHHCCC
GVEFRKLGEVCEIIRGKRVTKKEILDKGKYPVVSGGIGFMGYLNEYNREENTITIAQYGT
CCCHHHHHHHHHHHCCCCCCHHHHHCCCCCCEEECCCHHHHHHHHCCCCCCEEEEEECCC
AGFVNWQNQKFWANDVCFSVIPKETLINRYLYYVLTNMQNYLYSISNRSAIPYSISSNNI
CEEEECCCCCEECCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEECCCCE
MQITIPIPPLEIQQEIVKILDQFLALTTDLLAGIPAEIEARKKQYEYYREKLLTFKPLQN
EEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1691706 [H]