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The map label for this gene is hsdM [H]

Identifier: 188527305

GI number: 188527305

Start: 494332

End: 495921

Strand: Direct

Name: hsdM [H]

Synonym: HPSH_02560

Alternate gene names: 188527305

Gene position: 494332-495921 (Clockwise)

Preceding gene: 188527301

Following gene: 188527306

Centisome position: 30.73

GC content: 40.63

Gene sequence:

>1590_bases
ATGGAAAACAAAAACACTCAAGCGGATAAATCTTCTTCACTAGAACGCAACGAATTGCACAACACCATTTGGAAAGTGGC
TAATGAATTGAGAGGCTCAGTGGATGGCTGGGATTTTAAGCAATATGTTTTAGGCATTCTTTTTTACCGCTACATTTCAG
AAAACATGGCTCATTACATCAATAAAGAAGAGCGAAAGCGCGATCCGAGTTTTGATTACGCCAAATTAAGCGATGAAGAA
GCCGAGAGCGCAAAAGAAGGCCTGATTGAAGAAAAAGGCTTTTTCATCCCGCCAAGCGCTTTATTTTGTAATGTGCTAAA
AAACGCACCCCATAACGAAGATCTCAATGTAACCTTACAAAATATTTTTAACGAAATAGAAAAATCCAGCCTAGGGTTTA
AATCAGAAGAAAATGTCAAAGGCTTGTTTGCGGATTTAGATGTCAATAGCAATAAATTAGGGAGCTCTCATAAAAATAGG
GTTGAAAAATTGAATAAAATCCTTCAAGCCATAGGGGGCATGCAATTAGGCGACTACCAAAAAAGCGGGATTGATGTTTT
TGGCGACGCTTATGAATATTTAATGGCCATGTATGCGAGCAATGCCGGCAAAAGCGGAGGGGAATTTTTCACCCCCCAAG
AAGTGAGTGAATTACTCGCTAAAATCACCCTACACAACCAAGAGAGCGTCAATAAAGTTTATGACCCATGCTGTGGGAGC
GGATCGCTACTCTTACAATTTTCTAAAGTGTTAGGCGATAAAAACGTTTCAAAAGGGTATTTTGGGCAAGAAATCAATTT
GACCACTTACAACCTTTGCCATATCAACATGTTTTTGCATGACATCAATTACTCTAAATTCCACATAGCGCATGGGGACA
CGCTTTTAGATCCAAAACATGAAGACGATGAGCCTTTTGATGCGATCGTTTCCAACCCTCCTTATTCCACTAAATGGGCG
GGCAATAGCAACCCTATTTTAATCAACGACGAGCGCTTTAGCCCGGCCGGTGTGTTAGCGCCCAAAAACGCCGCCGATCT
CGCTTTCACCATGCACATGCTTTCTTATCTGTCCAATAGCGGCACTTGCGCGATCGTGGAATTTCCCGGGGTGCTTTATA
GGGGGAATGCTGAAGCAAAAATCAGAGAACATTTAGTCAAAGAGAATTTCATTGACTGCGTGATCGCTTTACCAGACAAC
CTCTTTTTTGGAACGAGTATCGCTACTTGTATTTTAGTGCTTAAGAAAAACAAACAAGACGACACCACGCTTTTTATTGA
TGCGAGTAAGGAATTTGTCAAAGAAGGCAAGAAAAACAAGCTCAAAGAACACAACCGAGAAAAGATTTTGCAAACCTATA
CTGAAAGGAAAACAATCAAACATTTTTCCGCCCTAGCCAATATGGAGAAAATCAAAGAAAACGATTACAACCTATCCGTG
AACCGCTTCGTGGAGCAAGAAGACACTAAAGAGATCATTGACATTAAAGCGCTCAATGGTGAGATTTCTCAAATCGTAGA
AAAACAAAGCGCTTTAAGGAACAGCCTTGAATCAATCATCAAAGAGTTAGAAGAGGGGCAAAATGCATAA

Upstream 100 bases:

>100_bases
GGTTAGCATTATTTAAGGCTTTAGTTATCGGTATTTGATTAAAATAAAACCTTTAAATTTTTAAAAAACACCAATCAAAA
CAAAAAAGAGGCTTATTATC

Downstream 100 bases:

>100_bases
AATAGAGCGCTTACTCCAAACTTTAGTGCCTAAGGGGGTGGAGTTTAAAACGCTTGAAGAGGTTTTTGAAATTAAAAATG
GTTACACCCCATCAAAAAAC

Product: type I restriction enzyme M protein (hsdM)

Products: NA

Alternate protein names: M.EcoR124II [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MENKNTQADKSSSLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMAHYINKEERKRDPSFDYAKLSDEE
AESAKEGLIEEKGFFIPPSALFCNVLKNAPHNEDLNVTLQNIFNEIEKSSLGFKSEENVKGLFADLDVNSNKLGSSHKNR
VEKLNKILQAIGGMQLGDYQKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGS
GSLLLQFSKVLGDKNVSKGYFGQEINLTTYNLCHINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWA
GNSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTCAIVEFPGVLYRGNAEAKIREHLVKENFIDCVIALPDN
LFFGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKEHNREKILQTYTERKTIKHFSALANMEKIKENDYNLSV
NRFVEQEDTKEIIDIKALNGEISQIVEKQSALRNSLESIIKELEEGQNA

Sequences:

>Translated_529_residues
MENKNTQADKSSSLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMAHYINKEERKRDPSFDYAKLSDEE
AESAKEGLIEEKGFFIPPSALFCNVLKNAPHNEDLNVTLQNIFNEIEKSSLGFKSEENVKGLFADLDVNSNKLGSSHKNR
VEKLNKILQAIGGMQLGDYQKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGS
GSLLLQFSKVLGDKNVSKGYFGQEINLTTYNLCHINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWA
GNSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTCAIVEFPGVLYRGNAEAKIREHLVKENFIDCVIALPDN
LFFGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKEHNREKILQTYTERKTIKHFSALANMEKIKENDYNLSV
NRFVEQEDTKEIIDIKALNGEISQIVEKQSALRNSLESIIKELEEGQNA
>Mature_529_residues
MENKNTQADKSSSLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMAHYINKEERKRDPSFDYAKLSDEE
AESAKEGLIEEKGFFIPPSALFCNVLKNAPHNEDLNVTLQNIFNEIEKSSLGFKSEENVKGLFADLDVNSNKLGSSHKNR
VEKLNKILQAIGGMQLGDYQKSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGS
GSLLLQFSKVLGDKNVSKGYFGQEINLTTYNLCHINMFLHDINYSKFHIAHGDTLLDPKHEDDEPFDAIVSNPPYSTKWA
GNSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNSGTCAIVEFPGVLYRGNAEAKIREHLVKENFIDCVIALPDN
LFFGTSIATCILVLKKNKQDDTTLFIDASKEFVKEGKKNKLKEHNREKILQTYTERKTIKHFSALANMEKIKENDYNLSV
NRFVEQEDTKEIIDIKALNGEISQIVEKQSALRNSLESIIKELEEGQNA

Specific function: Methylation of specific adenine residues; required for both restriction and modification activities. The EcoR124/3 I enzyme recognizes 5'-GAAN(7)RTCG-3' [H]

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=372, Percent_Identity=22.8494623655914, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR004546 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 59698; Mature: 59698

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENKNTQADKSSSLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMAHYI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NKEERKRDPSFDYAKLSDEEAESAKEGLIEEKGFFIPPSALFCNVLKNAPHNEDLNVTLQ
CHHHHCCCCCCCHHHCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEHH
NIFNEIEKSSLGFKSEENVKGLFADLDVNSNKLGSSHKNRVEKLNKILQAIGGMQLGDYQ
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
KSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
GSLLLQFSKVLGDKNVSKGYFGQEINLTTYNLCHINMFLHDINYSKFHIAHGDTLLDPKH
CHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEEEEEEHEECCCCEEEEECCCCCCCCCC
EDDEPFDAIVSNPPYSTKWAGNSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNS
CCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GTCAIVEFPGVLYRGNAEAKIREHLVKENFIDCVIALPDNLFFGTSIATCILVLKKNKQD
CCEEEEECCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
DTTLFIDASKEFVKEGKKNKLKEHNREKILQTYTERKTIKHFSALANMEKIKENDYNLSV
CCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
NRFVEQEDTKEIIDIKALNGEISQIVEKQSALRNSLESIIKELEEGQNA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MENKNTQADKSSSLERNELHNTIWKVANELRGSVDGWDFKQYVLGILFYRYISENMAHYI
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
NKEERKRDPSFDYAKLSDEEAESAKEGLIEEKGFFIPPSALFCNVLKNAPHNEDLNVTLQ
CHHHHCCCCCCCHHHCCCHHHHHHHHCCHHCCCCCCCHHHHHHHHHHCCCCCCCCEEEHH
NIFNEIEKSSLGFKSEENVKGLFADLDVNSNKLGSSHKNRVEKLNKILQAIGGMQLGDYQ
HHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCC
KSGIDVFGDAYEYLMAMYASNAGKSGGEFFTPQEVSELLAKITLHNQESVNKVYDPCCGS
CCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCC
GSLLLQFSKVLGDKNVSKGYFGQEINLTTYNLCHINMFLHDINYSKFHIAHGDTLLDPKH
CHHHHHHHHHHCCCCCCCCCCCCEECCEEEEEEEEEEEHEECCCCEEEEECCCCCCCCCC
EDDEPFDAIVSNPPYSTKWAGNSNPILINDERFSPAGVLAPKNAADLAFTMHMLSYLSNS
CCCCCHHHHHCCCCCCCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
GTCAIVEFPGVLYRGNAEAKIREHLVKENFIDCVIALPDNLFFGTSIATCILVLKKNKQD
CCEEEEECCCEEEECCCHHHHHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCC
DTTLFIDASKEFVKEGKKNKLKEHNREKILQTYTERKTIKHFSALANMEKIKENDYNLSV
CCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEH
NRFVEQEDTKEIIDIKALNGEISQIVEKQSALRNSLESIIKELEEGQNA
HHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2784505 [H]