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The map label for this gene is ngoPIIM [H]

Identifier: 188527304

GI number: 188527304

Start: 493165

End: 494148

Strand: Reverse

Name: ngoPIIM [H]

Synonym: HPSH_02555

Alternate gene names: 188527304

Gene position: 494148-493165 (Counterclockwise)

Preceding gene: 188527311

Following gene: 188527303

Centisome position: 30.72

GC content: 34.86

Gene sequence:

>984_bases
TTGAATTTACTTTCATTATTTGCTGGAGCCGGAGGGCTAGATTTAGGTTTTGAGAAAGCAGGGTTTAAAATAGTAGTTGC
AAATGAATATGATAAAAATATTACACCTACTTACAGACTAAATCATAAAAATACACGACTACTAGAAAAAGACATTAAAA
ATTTACAAACCAGTGAAATTAACTTTTCTGTTGATGGGATTATTGGCGGTCCGCCATGCCAAAGTTGGTCAGAGGCTGGG
AATTTAAAAGGTATTGATGATGCTAGAGGGCAGTTATTTTATGAATATTTGCGCCTTTTAAGAGAGCTTAAGCCTAAATT
TTTTTTAGCTGAAAATGTAAGGGGCATGCTCGCTCAACGCCACGAAACGAGTGTGAAAAATATCCTTAATGCTTTTAAAG
AGTGTGGTTATGAAGTCAATACGCATTTAGTGAATGCCAAAGATTATGGAGTAGCGCAAGAAAGGCTTAGGGTTTTTTAT
ATTGGTTTTAGGAAAGATTTAAAAATCAATTACATTTTTCCTAAAGGTTCAACGCATTTAAAAAAACTCACTTTAAAAGA
TGTTATTTGGGATCTAAAAGATAGCGTGGTGTGCGCTTTAGCAAAAAACAAACGAAACCCTAATGCCATTAACAATCATG
AATATTTTATTGGTTCTTATTCGCCGATCTTTATGAGCAGAAATCGTGTAAAAAATTGGGACGAACAAGCCTTTACTATA
CAAGCATCAGGAAGGCAATGCCAACTACACCCACAAGCCCCTAAAATGGCTAAATTTGGCAAAAATGATTGCAGATTTAT
AGAAAATTACCAACATTTATACCGCCGCTTGAGCGTGCGAGAGTGTGCAAGGATTCAAGGTTTTGATGATGATTTTTATT
TCGTTTATGAAAACCTCAACGACGCTTATAAAATGATAGGTAATGCTGTGCCTGTGAGTTTAGCTAAAGAAATTGCTGTA
AGTATTTATAAGGTGTTATATTGA

Upstream 100 bases:

>100_bases
CTTAAATAATGCTAACCCATTCTTTAGAATAACCCGATACTTATTGTAAAATTCTTTCATTATAAAGTATTGAGATTTAA
CAGAATGATAAGGATAGTTT

Downstream 100 bases:

>100_bases
GCGATAAGAGCAATAATCAAGGTAGGGCGTATGAATACGCATGGTGTTTAGCTTTGGAGCAAAAATTGAGCGTTTTTAAA
AAGGTTATAGTGGATAAACA

Product: site-specific DNA methyltransferase

Products: NA

Alternate protein names: M.NgoPII; Cytosine-specific methyltransferase NgoPII [H]

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MNLLSLFAGAGGLDLGFEKAGFKIVVANEYDKNITPTYRLNHKNTRLLEKDIKNLQTSEINFSVDGIIGGPPCQSWSEAG
NLKGIDDARGQLFYEYLRLLRELKPKFFLAENVRGMLAQRHETSVKNILNAFKECGYEVNTHLVNAKDYGVAQERLRVFY
IGFRKDLKINYIFPKGSTHLKKLTLKDVIWDLKDSVVCALAKNKRNPNAINNHEYFIGSYSPIFMSRNRVKNWDEQAFTI
QASGRQCQLHPQAPKMAKFGKNDCRFIENYQHLYRRLSVRECARIQGFDDDFYFVYENLNDAYKMIGNAVPVSLAKEIAV
SIYKVLY

Sequences:

>Translated_327_residues
MNLLSLFAGAGGLDLGFEKAGFKIVVANEYDKNITPTYRLNHKNTRLLEKDIKNLQTSEINFSVDGIIGGPPCQSWSEAG
NLKGIDDARGQLFYEYLRLLRELKPKFFLAENVRGMLAQRHETSVKNILNAFKECGYEVNTHLVNAKDYGVAQERLRVFY
IGFRKDLKINYIFPKGSTHLKKLTLKDVIWDLKDSVVCALAKNKRNPNAINNHEYFIGSYSPIFMSRNRVKNWDEQAFTI
QASGRQCQLHPQAPKMAKFGKNDCRFIENYQHLYRRLSVRECARIQGFDDDFYFVYENLNDAYKMIGNAVPVSLAKEIAV
SIYKVLY
>Mature_327_residues
MNLLSLFAGAGGLDLGFEKAGFKIVVANEYDKNITPTYRLNHKNTRLLEKDIKNLQTSEINFSVDGIIGGPPCQSWSEAG
NLKGIDDARGQLFYEYLRLLRELKPKFFLAENVRGMLAQRHETSVKNILNAFKECGYEVNTHLVNAKDYGVAQERLRVFY
IGFRKDLKINYIFPKGSTHLKKLTLKDVIWDLKDSVVCALAKNKRNPNAINNHEYFIGSYSPIFMSRNRVKNWDEQAFTI
QASGRQCQLHPQAPKMAKFGKNDCRFIENYQHLYRRLSVRECARIQGFDDDFYFVYENLNDAYKMIGNAVPVSLAKEIAV
SIYKVLY

Specific function: This methylase recognizes the double-stranded sequence GGCC, causes specific methylation on C-3 on both strands, and protects the DNA from cleavage by the NgoPII endonuclease [H]

COG id: COG0270

COG function: function code L; Site-specific DNA methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the C5-methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4758184, Length=168, Percent_Identity=28.5714285714286, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI195927037, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI4503351, Length=192, Percent_Identity=27.6041666666667, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788271, Length=360, Percent_Identity=25.5555555555556, Blast_Score=89, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018117
- InterPro:   IPR001525 [H]

Pfam domain/function: PF00145 DNA_methylase [H]

EC number: =2.1.1.37 [H]

Molecular weight: Translated: 37625; Mature: 37625

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: PS00094 C5_MTASE_1 ; PS00095 C5_MTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLLSLFAGAGGLDLGFEKAGFKIVVANEYDKNITPTYRLNHKNTRLLEKDIKNLQTSEI
CCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCE
NFSVDGIIGGPPCQSWSEAGNLKGIDDARGQLFYEYLRLLRELKPKFFLAENVRGMLAQR
EEEECCEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
HETSVKNILNAFKECGYEVNTHLVNAKDYGVAQERLRVFYIGFRKDLKINYIFPKGSTHL
HHHHHHHHHHHHHHCCCEEEEEEECCHHCCHHHHCEEEEEEEEECCEEEEEEECCCCCHH
KKLTLKDVIWDLKDSVVCALAKNKRNPNAINNHEYFIGSYSPIFMSRNRVKNWDEQAFTI
HHHHHHHHHHHHHHHHEEEEHHCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCEEEE
QASGRQCQLHPQAPKMAKFGKNDCRFIENYQHLYRRLSVRECARIQGFDDDFYFVYENLN
EECCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
DAYKMIGNAVPVSLAKEIAVSIYKVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNLLSLFAGAGGLDLGFEKAGFKIVVANEYDKNITPTYRLNHKNTRLLEKDIKNLQTSEI
CCHHHHHCCCCCCCCCCCCCCCEEEEECCCCCCCCCCEEECCCHHHHHHHHHHCCCCCCE
NFSVDGIIGGPPCQSWSEAGNLKGIDDARGQLFYEYLRLLRELKPKFFLAENVRGMLAQR
EEEECCEECCCCCCCHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHH
HETSVKNILNAFKECGYEVNTHLVNAKDYGVAQERLRVFYIGFRKDLKINYIFPKGSTHL
HHHHHHHHHHHHHHCCCEEEEEEECCHHCCHHHHCEEEEEEEEECCEEEEEEECCCCCHH
KKLTLKDVIWDLKDSVVCALAKNKRNPNAINNHEYFIGSYSPIFMSRNRVKNWDEQAFTI
HHHHHHHHHHHHHHHHEEEEHHCCCCCCCCCCCCEEEECCCHHHHCCCCCCCCCCCEEEE
QASGRQCQLHPQAPKMAKFGKNDCRFIENYQHLYRRLSVRECARIQGFDDDFYFVYENLN
EECCCEEEECCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
DAYKMIGNAVPVSLAKEIAVSIYKVLY
HHHHHHCCCCCHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2837733; 2501649; 8406039 [H]