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The map label for this gene is tgt

Identifier: 188527087

GI number: 188527087

Start: 273744

End: 274859

Strand: Reverse

Name: tgt

Synonym: HPSH_01460

Alternate gene names: 188527087

Gene position: 274859-273744 (Counterclockwise)

Preceding gene: 188527101

Following gene: 188527067

Centisome position: 17.09

GC content: 43.73

Gene sequence:

>1116_bases
ATGGATTTTCAACTCCAAGCGACGGACAATAACGCGCGAGCTGGTCTTTTAAATTTAGCCCATTCTCAAGTGGCAACGCC
TGTTTTTATGCCAGTAGGCACGCAAGGCTGTATCAAATCTTTAGACGCTACTGACATGCAAGGCATTTTAGGCGCTAAAC
TCATTTTAGCCAACACCTATCACTTGTATTTAAGGCCGGGCGAAAAGGTCGTTGAGGAGTTAGGGGGCTTGCATCGTTTC
GCTCAATTTCATGGGAGTTTTTTAACCGATAGTGGAGGGTTTCAAGCCTTTAGTTTGAGCGATAATGTCAAATTGCAAGA
AGACGGGATTGTTTTTAAATCCCATATTGATGGGAGCAAGCATTTATTCACGCCCGCTAAAGTTTTGGACATTCAATATT
CTTTAAATAGCGATATTATGATGGTTTTAGACGATTTAGTGGGCTTGCCCGCTCCCTTAAAACGCCTTGAAGAATCCATT
AAAAGAAGCGCTAAATGGGCGAATCTTAGCCTAGAATACCACAAGCAAAAAAACCGCCCCAACAACAACCTTTTTGCCAT
TATCCAAGGCGGGACGCATTTGAAAATGCGCAGCCTTAGCGTGGGATTAACGCATGAGGGTTTTGATGGCTACGCTATAG
GCGGTTTAGCGGTGGGGGAAAGCGCTGATGAAATGCTAGAAACCATCGCGCACACCGCCCCCTTGCTCCCCAAAGACAAG
CCTCGCTACTTAATGGGCGTAGGCACGCCTGAAAATATCCTAGACGCTATCGGTTTAGGGGTGGATATGTTTGATTGCGT
GATGCCCACCAGAAACGCCAGAAACGCCACCCTTTTCACGCATTCTGGCAAAATTTCTATCAAAAACGCGCCCTATAAAT
TGGATGATACCCCTATTGAAGAAAATTGCGCATGTTATACTTGCAAACGCTATTCTAAAGCCTATTTGCACCATTTGTTT
AGGGCTAAAGAACTCACTTACGCTCGTTTGGCCAGCTTGCACAATTTGCATTTTTATTTAGAGCTGGTTAAAAACGCCAG
AAACGCCATTTTAGAAAAGCGGTTTTTGAGTTTTAAAAAAGAATTTTTAAAGAAATACCACTCCCGCTCTCATTGA

Upstream 100 bases:

>100_bases
TTTTTTGCTACAATGCCATCTAAAATAAGGGCGATTTTTTTCAATTCCAAACTTCCTAAAAAATGATTACGCCATTATAT
CCAAGATTAAGGCTTAAACG

Downstream 100 bases:

>100_bases
ATGAGTTTTTCTTCAAATAAGGCTTTAGTTTTTAATTTTTAATTAAAACAAAACCCTATCTTTGATAAATCTCAGGATGG
GTGCTTTTAAAACGCCTGTG

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDMQGILGAKLILANTYHLYLRPGEKVVEELGGLHRF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPNNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDDTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLKKYHSRSH

Sequences:

>Translated_371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDMQGILGAKLILANTYHLYLRPGEKVVEELGGLHRF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPNNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDDTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLKKYHSRSH
>Mature_371_residues
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDMQGILGAKLILANTYHLYLRPGEKVVEELGGLHRF
AQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSKHLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESI
KRSAKWANLSLEYHKQKNRPNNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDDTPIEENCACYTCKRYSKAYLHHLF
RAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKKEFLKKYHSRSH

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=364, Percent_Identity=40.3846153846154, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1786607, Length=367, Percent_Identity=46.866485013624, Blast_Score=350, Evalue=1e-97,
Organism=Caenorhabditis elegans, GI17544680, Length=369, Percent_Identity=38.4823848238482, Blast_Score=252, Evalue=3e-67,
Organism=Caenorhabditis elegans, GI25146502, Length=213, Percent_Identity=27.2300469483568, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=40.8839779005525, Blast_Score=299, Evalue=2e-81,
Organism=Drosophila melanogaster, GI21355311, Length=255, Percent_Identity=28.2352941176471, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41391; Mature: 41391

Theoretical pI: Translated: 8.85; Mature: 8.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDMQGILGAKLILANTY
CCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCHHHHHHHHHEEEEEEEE
HLYLRPGEKVVEELGGLHRFAQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSK
EEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANLSLEYHKQKNRP
CEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCEEEEHHHCCCC
NNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDDTPIE
CCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCACYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLKKYHSRSH
HHHHHHHCCCC
>Mature Secondary Structure
MDFQLQATDNNARAGLLNLAHSQVATPVFMPVGTQGCIKSLDATDMQGILGAKLILANTY
CCEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCHHHHHHHHHEEEEEEEE
HLYLRPGEKVVEELGGLHRFAQFHGSFLTDSGGFQAFSLSDNVKLQEDGIVFKSHIDGSK
EEEECCCHHHHHHHHHHHHHHHHCCCEEECCCCEEEEECCCCCEEECCCEEEEECCCCCC
HLFTPAKVLDIQYSLNSDIMMVLDDLVGLPAPLKRLEESIKRSAKWANLSLEYHKQKNRP
CEECCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHHHHCCHHHCCEEEEHHHCCCC
NNNLFAIIQGGTHLKMRSLSVGLTHEGFDGYAIGGLAVGESADEMLETIAHTAPLLPKDK
CCCEEEEEECCCEEEEEEEEEECEECCCCCEEECCEEECCCHHHHHHHHHHHCCCCCCCC
PRYLMGVGTPENILDAIGLGVDMFDCVMPTRNARNATLFTHSGKISIKNAPYKLDDTPIE
CCEEEECCCHHHHHHHHHCCHHHHHHHCCCCCCCCEEEEEECCEEEECCCCEECCCCCCC
ENCACYTCKRYSKAYLHHLFRAKELTYARLASLHNLHFYLELVKNARNAILEKRFLSFKK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EFLKKYHSRSH
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA