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The map label for this gene is hemB [H]

Identifier: 188526968

GI number: 188526968

Start: 156273

End: 157244

Strand: Reverse

Name: hemB [H]

Synonym: HPSH_00825

Alternate gene names: 188526968

Gene position: 157244-156273 (Counterclockwise)

Preceding gene: 188526969

Following gene: 188526967

Centisome position: 9.78

GC content: 39.4

Gene sequence:

>972_bases
ATGTTCAAACGATTGAGAAGATTACGAAGCAGCGAAAACTTAAGAGCGATGATAAGAGAAACGCGTCTGAATATTGATGA
TTTCATCGCTCCCTTATTTGTCATAGAAAGCGATAGTTGTATTAAAAATGAAATCAACTCCATGCCTGGCGTTTATCAAA
TGAGTATAGAGCCTCTTTTAAAAGAATGCGAAGAATTAGTGGGTTTAGGCATCAAAGCCGTTTTATTGTTTGGCATTCCT
AAACATAAGGATGCTACAGGAAGCCATGCGTTAAATAAGGATCACATTGTCGCAAAAGCCGCTAGAGCGATTAAAAAACG
ATTCAAGGATTTAATCGTTATAGCGGATTTGTGTTTTTGTGAATACACCGACCATGGGCATTGCGGGATTTTAGAAAACG
CTTCTGTGTCTAACGATAAAACGCTAGAGATTTTAAACCTTCAAGGGCTTATTTTGGCTGAAAGCGGTGTGGATATTCTA
GCCCCAAGCAACATGATGGATGGGAATGTTTTAAGCTTGAGAAAAACGCTAGATAACGCCGGCTATACCCACACGCCCAT
CATGAGCTATTCCACTAAATTTGCGAGCAGTTACTATGGGCCTTTTAGAGATGTAGCAAACTCTGCACCGAGTTTTGGCA
ATCGCAAAAGCTATCAAATGGATTACGCTAATAAAAAAGAAGCGCTTTTAGAAAGCTTAGAAGATGAAAAACAGGGTGCG
GATATTTTAATGGTGAAACCGGCTTTGGCGTATTTGGATATTGTTAAAGAAATCAGAGATCACACTTTGCTCCCTTTAGC
GCTCTATAATGTGAGCGGGGAATACGCCATGCTCAAACTCGCTCAAAAACACAACCTGATCAACTATGAAAGCGCTTTAT
TAGAAACGATGACTTGTTTTAAAAGAGCGGGAGCGGATATGATCATTAGCTATCATGCTAAAGAAATGGCTAACTTATTA
CAAAGGAATTGA

Upstream 100 bases:

>100_bases
GAGCTACCCCCCCCCCCCCGAAAATTTGAGAGAGGTGAGCGGAATGAAGGGGATAGAAAAAGCCAATTATGGGGTTAAAG
AAAAACAATAAAGAGAGAAC

Downstream 100 bases:

>100_bases
ATGGGACGAGCGTTTGAATACAGAAGAGCGGCTAAAGAAAAACGATGGGATAAGATGAGTAAGGTTTTCCCCAAGCTCGC
CAAAGCGATCACTCTAGCGG

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MFKRLRRLRSSENLRAMIRETRLNIDDFIAPLFVIESDSCIKNEINSMPGVYQMSIEPLLKECEELVGLGIKAVLLFGIP
KHKDATGSHALNKDHIVAKAARAIKKRFKDLIVIADLCFCEYTDHGHCGILENASVSNDKTLEILNLQGLILAESGVDIL
APSNMMDGNVLSLRKTLDNAGYTHTPIMSYSTKFASSYYGPFRDVANSAPSFGNRKSYQMDYANKKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRDHTLLPLALYNVSGEYAMLKLAQKHNLINYESALLETMTCFKRAGADMIISYHAKEMANLL
QRN

Sequences:

>Translated_323_residues
MFKRLRRLRSSENLRAMIRETRLNIDDFIAPLFVIESDSCIKNEINSMPGVYQMSIEPLLKECEELVGLGIKAVLLFGIP
KHKDATGSHALNKDHIVAKAARAIKKRFKDLIVIADLCFCEYTDHGHCGILENASVSNDKTLEILNLQGLILAESGVDIL
APSNMMDGNVLSLRKTLDNAGYTHTPIMSYSTKFASSYYGPFRDVANSAPSFGNRKSYQMDYANKKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRDHTLLPLALYNVSGEYAMLKLAQKHNLINYESALLETMTCFKRAGADMIISYHAKEMANLL
QRN
>Mature_323_residues
MFKRLRRLRSSENLRAMIRETRLNIDDFIAPLFVIESDSCIKNEINSMPGVYQMSIEPLLKECEELVGLGIKAVLLFGIP
KHKDATGSHALNKDHIVAKAARAIKKRFKDLIVIADLCFCEYTDHGHCGILENASVSNDKTLEILNLQGLILAESGVDIL
APSNMMDGNVLSLRKTLDNAGYTHTPIMSYSTKFASSYYGPFRDVANSAPSFGNRKSYQMDYANKKEALLESLEDEKQGA
DILMVKPALAYLDIVKEIRDHTLLPLALYNVSGEYAMLKLAQKHNLINYESALLETMTCFKRAGADMIISYHAKEMANLL
QRN

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=306, Percent_Identity=41.5032679738562, Blast_Score=245, Evalue=4e-65,
Organism=Escherichia coli, GI87081728, Length=318, Percent_Identity=50.9433962264151, Blast_Score=315, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6321398, Length=313, Percent_Identity=36.4217252396166, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI21358291, Length=302, Percent_Identity=36.7549668874172, Blast_Score=217, Evalue=7e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 36199; Mature: 36199

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRLRRLRSSENLRAMIRETRLNIDDFIAPLFVIESDSCIKNEINSMPGVYQMSIEPLL
CHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEHHHHHHH
KECEELVGLGIKAVLLFGIPKHKDATGSHALNKDHIVAKAARAIKKRFKDLIVIADLCFC
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGILENASVSNDKTLEILNLQGLILAESGVDILAPSNMMDGNVLSLRKTLDNA
CCCCCCCCCEECCCCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCHHHHHHHHHHCC
GYTHTPIMSYSTKFASSYYGPFRDVANSAPSFGNRKSYQMDYANKKEALLESLEDEKQGA
CCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCC
DILMVKPALAYLDIVKEIRDHTLLPLALYNVSGEYAMLKLAQKHNLINYESALLETMTCF
CEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
KRAGADMIISYHAKEMANLLQRN
HHCCCHHEEHHHHHHHHHHHHCC
>Mature Secondary Structure
MFKRLRRLRSSENLRAMIRETRLNIDDFIAPLFVIESDSCIKNEINSMPGVYQMSIEPLL
CHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHEEECCCHHHHHHHHCCCCEEEHHHHHHH
KECEELVGLGIKAVLLFGIPKHKDATGSHALNKDHIVAKAARAIKKRFKDLIVIADLCFC
HHHHHHHCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EYTDHGHCGILENASVSNDKTLEILNLQGLILAESGVDILAPSNMMDGNVLSLRKTLDNA
CCCCCCCCCEECCCCCCCCCEEEEEECCEEEEECCCCCEECCCCCCCCHHHHHHHHHHCC
GYTHTPIMSYSTKFASSYYGPFRDVANSAPSFGNRKSYQMDYANKKEALLESLEDEKQGA
CCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCEECCCCCHHHHHHHHHHHHHCCC
DILMVKPALAYLDIVKEIRDHTLLPLALYNVSGEYAMLKLAQKHNLINYESALLETMTCF
CEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHCCCCHHHHHHHHHHHHH
KRAGADMIISYHAKEMANLLQRN
HHCCCHHEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]