Click here to switch to the map view.

The map label for this gene is serA [C]

Identifier: 188526901

GI number: 188526901

Start: 87562

End: 88506

Strand: Direct

Name: serA [C]

Synonym: HPSH_00480

Alternate gene names: 188526901

Gene position: 87562-88506 (Clockwise)

Preceding gene: 188526898

Following gene: 188526904

Centisome position: 5.44

GC content: 40.63

Gene sequence:

>945_bases
ATGGAAACATTCAAAAAAGGCGTGGTCTTAGACGCTAAAAGCGTGGGGTTAAAAGCGCTAGAAGTTTTAAAAGAAGTGGC
GGATTTTGATTTTTATGGGATCACTTCGCCTAATCAAGTGGTTGAGCGCTGCAAAGACGCTGAGATTGTGGTGCTGAATA
AAGTCGTTATCGCTCAAGAAATTTTAAGCCAATTGCCTAAACTCAAACTCATTTGCATCACCGCTACAGGCACGGATAAT
GTAGATATAAAAAGCGCGAAAGCTTTAGGCATAGAAGTCAAAAATGTGAGCGCTTATTCTACAGAATCCGTAGCCCAGCA
CACTTTAGCGTGCGCGTTGTCTTTGCTAGGGAGGATCAATGATTACGATCATTATTGCAAAAGCGGGGAATATAGTCAAA
GCGATATTTTTACGCACATTAGCGATATTAAAATGGGGCTTATTAAAGGGAGTCAATGGGGGGTTATTGGTTTAGGGACT
ATTGGTAAAAGAGTCGCCAAGCTCGCTCAAACTTTTGGGGCAAAGGTGGTGTATTATTCCCCTAAAGACAAAAAAGAAGA
GTATGAGCGCTTGAGTTTAAAAGATTTGCTCACAACAAGCGATATTATCAGCATTCATGCCCCCCTAAATGAAAGCACGC
GCGATTTAATCGCTCTAAAGGAATTGCAAAGCCTAAAAGACGGGGCGATTTTAATCAATGTGGGGCGTGGGGGCATTGTG
AATGAAAAGGATCTGGCTGGAATTTTAGAAACCAAAGATTTGTATTATGCGAGCGATGTGTTTGTGAAAGAGCCTTTTGA
AAAAGATCATGCGTTTTTGAACCCAAAGATCCAAAATAAATTGCTTTTAACCCCCCATATCGCATGGGCGTATAGCGACA
GCTTGAAAACCTTAGTGGAAAAAACCAAAGAAAATATCCAGGATTTTTTAGCTTCTCAAAAATAA

Upstream 100 bases:

>100_bases
GGAGGGTGTTTTTCTTGGGTTTTGGCATGCTTAGTTTCTTTTAAAAAAGGATTTTGTGGTTTAATTATATAACTTTAAAA
CTAATTCTAGGAGTAAAGAG

Downstream 100 bases:

>100_bases
ACGCAAAAAAGGGGGTTTAATTGTTGGGCTTTTCTCCCCTTTTGTCGTGAGAAGCGCCCCTTTCTAAAGCAAGGGACTTC
CTAACTAAAGCATTCCATTG

Product: 2-hydroxyacid dehydrogenase

Products: 3-phosphohydroxypyruvate; NADH; H+

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
METFKKGVVLDAKSVGLKALEVLKEVADFDFYGITSPNQVVERCKDAEIVVLNKVVIAQEILSQLPKLKLICITATGTDN
VDIKSAKALGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDHYCKSGEYSQSDIFTHISDIKMGLIKGSQWGVIGLGT
IGKRVAKLAQTFGAKVVYYSPKDKKEEYERLSLKDLLTTSDIISIHAPLNESTRDLIALKELQSLKDGAILINVGRGGIV
NEKDLAGILETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK

Sequences:

>Translated_314_residues
METFKKGVVLDAKSVGLKALEVLKEVADFDFYGITSPNQVVERCKDAEIVVLNKVVIAQEILSQLPKLKLICITATGTDN
VDIKSAKALGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDHYCKSGEYSQSDIFTHISDIKMGLIKGSQWGVIGLGT
IGKRVAKLAQTFGAKVVYYSPKDKKEEYERLSLKDLLTTSDIISIHAPLNESTRDLIALKELQSLKDGAILINVGRGGIV
NEKDLAGILETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK
>Mature_314_residues
METFKKGVVLDAKSVGLKALEVLKEVADFDFYGITSPNQVVERCKDAEIVVLNKVVIAQEILSQLPKLKLICITATGTDN
VDIKSAKALGIEVKNVSAYSTESVAQHTLACALSLLGRINDYDHYCKSGEYSQSDIFTHISDIKMGLIKGSQWGVIGLGT
IGKRVAKLAQTFGAKVVYYSPKDKKEEYERLSLKDLLTTSDIISIHAPLNESTRDLIALKELQSLKDGAILINVGRGGIV
NEKDLAGILETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVEKTKENIQDFLASQK

Specific function: Serine biosynthesis; first step. [C]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI61743967, Length=309, Percent_Identity=34.3042071197411, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI4557497, Length=309, Percent_Identity=34.3042071197411, Blast_Score=138, Evalue=7e-33,
Organism=Homo sapiens, GI145580578, Length=296, Percent_Identity=32.4324324324324, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI4557499, Length=296, Percent_Identity=32.4324324324324, Blast_Score=128, Evalue=6e-30,
Organism=Homo sapiens, GI145580575, Length=296, Percent_Identity=32.4324324324324, Blast_Score=128, Evalue=8e-30,
Organism=Homo sapiens, GI23308577, Length=261, Percent_Identity=27.9693486590038, Blast_Score=101, Evalue=1e-21,
Organism=Homo sapiens, GI6912396, Length=238, Percent_Identity=27.3109243697479, Blast_Score=82, Evalue=7e-16,
Organism=Escherichia coli, GI1789279, Length=286, Percent_Identity=29.7202797202797, Blast_Score=111, Evalue=6e-26,
Organism=Escherichia coli, GI87082289, Length=258, Percent_Identity=28.2945736434109, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787645, Length=303, Percent_Identity=25.0825082508251, Blast_Score=96, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI25147481, Length=302, Percent_Identity=28.476821192053, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI17532191, Length=275, Percent_Identity=32.7272727272727, Blast_Score=115, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6324964, Length=304, Percent_Identity=27.6315789473684, Blast_Score=107, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6322116, Length=296, Percent_Identity=24.6621621621622, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320925, Length=296, Percent_Identity=24.6621621621622, Blast_Score=93, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6324055, Length=257, Percent_Identity=24.5136186770428, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6324980, Length=138, Percent_Identity=31.1594202898551, Blast_Score=65, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24646446, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646448, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646452, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI24646450, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=3e-31,
Organism=Drosophila melanogaster, GI62472511, Length=303, Percent_Identity=31.6831683168317, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI28574286, Length=305, Percent_Identity=27.5409836065574, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921140, Length=262, Percent_Identity=26.7175572519084, Blast_Score=95, Evalue=5e-20,
Organism=Drosophila melanogaster, GI28571528, Length=277, Percent_Identity=28.8808664259928, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24585516, Length=299, Percent_Identity=26.0869565217391, Blast_Score=81, Evalue=1e-15,
Organism=Drosophila melanogaster, GI45552429, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585514, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574282, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI28574284, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI45551003, Length=256, Percent_Identity=24.609375, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: 1.1.1.95

Molecular weight: Translated: 34666; Mature: 34666

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METFKKGVVLDAKSVGLKALEVLKEVADFDFYGITSPNQVVERCKDAEIVVLNKVVIAQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHH
ILSQLPKLKLICITATGTDNVDIKSAKALGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DYDHYCKSGEYSQSDIFTHISDIKMGLIKGSQWGVIGLGTIGKRVAKLAQTFGAKVVYYS
CHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYERLSLKDLLTTSDIISIHAPLNESTRDLIALKELQSLKDGAILINVGRGGIV
CCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
NEKDLAGILETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVE
CCHHHHHHHHHCCCEEECCHHEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLASQK
HHHHHHHHHHHCCC
>Mature Secondary Structure
METFKKGVVLDAKSVGLKALEVLKEVADFDFYGITSPNQVVERCKDAEIVVLNKVVIAQE
CCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCEEEEHHHHHHHH
ILSQLPKLKLICITATGTDNVDIKSAKALGIEVKNVSAYSTESVAQHTLACALSLLGRIN
HHHHCCCEEEEEEEECCCCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCC
DYDHYCKSGEYSQSDIFTHISDIKMGLIKGSQWGVIGLGTIGKRVAKLAQTFGAKVVYYS
CHHHHHCCCCCCHHHHHHHHHHHHHHEEECCCCCEEEECHHHHHHHHHHHHHCCEEEEEC
PKDKKEEYERLSLKDLLTTSDIISIHAPLNESTRDLIALKELQSLKDGAILINVGRGGIV
CCCHHHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCC
NEKDLAGILETKDLYYASDVFVKEPFEKDHAFLNPKIQNKLLLTPHIAWAYSDSLKTLVE
CCHHHHHHHHHCCCEEECCHHEECCCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHH
KTKENIQDFLASQK
HHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-phosphoglycerate; NAD+

Specific reaction: 3-phosphoglycerate + NAD+ = 3-phosphohydroxypyruvate + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800; 10675023 [H]