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The map label for this gene is mboIBM [H]

Identifier: 188526898

GI number: 188526898

Start: 85119

End: 85952

Strand: Direct

Name: mboIBM [H]

Synonym: HPSH_00465

Alternate gene names: 188526898

Gene position: 85119-85952 (Clockwise)

Preceding gene: 188526887

Following gene: 188526901

Centisome position: 5.29

GC content: 40.17

Gene sequence:

>834_bases
ATGGTAATCGTGCATTCTAATGAAATCGCACGCCCCATTTTTAAAAGCCAAGACCAGCTTTTCACCCTTTATCAAGGGGA
TTGCAATGAGGTTTTGCCCCAATTTGAAAACCAGTTTGATTTGATTTTTGCCGATCCGCCTTATTTCCTTTCTAATGACG
GCTTAAGCATACAGAGCGGTAAAATCGTGAGCGTCAATAAGGGCGATTGGGATAAAGAAGAGAGCGTCAATGATATTGAT
GAGTTCAATTACCAGTGGATAAATAACGCCAAAAAGGCTTTAAAAAACACAGGAAGCCTTTTAATCAGTGGGACTTACCA
CAATATCTTTTCTTTGGGGCGTATTTTACAAAAATTGGATTTTAAGATCTTAAACCTCATCACCTGGCAAAAAACCAACC
CTCCGCCCAATTTTAGCTGCCGCTATTTGACGCATTCAGCTGAGCAAATCATTTGGGCGAGAAAAAGCCACAAACATAAA
CATGTTTTTAACTATGAGGTTTTAAAAAAGATCAATAACGACAAGCAAATGCGCGATGTGTGGAGCTTCCCAGCGATCGC
TCCTTGGGAAAAAGCTAATGGCAAGCACCCCACTCAAAAACCCCTCGCTTTATTGGTGCGCTTGCTTTTAATGGCGAGCG
ATGAAAATTCCCTCATTGGCGATCCTTTTAGCGGGAGCTCTACCACAGGCATTGCGGCTAATCTTTTGGAGAGGCAATTT
ATCGGTATAGAAAAAGAAAGCGAGTTTGTCAAAATATCAATGGATAGGAAATTAGAATTAGACGCTCGCTTTAAAGAAAT
CCGATCTAAAATCAAAGATTTAAATCTTAAGTAA

Upstream 100 bases:

>100_bases
TAGGTTGGCTAGACGCTAAAAGCAAACTTCAAGAAGCTTACAAATCTGTAGAAATCTACAATTTGAGCAATGTAAATGAT
TTTATATCAAAGGCGCAAAA

Downstream 100 bases:

>100_bases
GCCTTTTTTAAGCCGCTTTAAGCGTTATACTTTTCGGATTTTACCTCAAAATGGGATTCTATTTTCACCCATTTCTCACA
AAGGATATTTTCATGCCCAA

Product: type II restriction enzyme M protein (hsdM)

Products: NA

Alternate protein names: M.MboIB; Adenine-specific methyltransferase MboIB; M.MboI C [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MVIVHSNEIARPIFKSQDQLFTLYQGDCNEVLPQFENQFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEESVNDID
EFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHK
HVFNYEVLKKINNDKQMRDVWSFPAIAPWEKANGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLERQF
IGIEKESEFVKISMDRKLELDARFKEIRSKIKDLNLK

Sequences:

>Translated_277_residues
MVIVHSNEIARPIFKSQDQLFTLYQGDCNEVLPQFENQFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEESVNDID
EFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHK
HVFNYEVLKKINNDKQMRDVWSFPAIAPWEKANGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLERQF
IGIEKESEFVKISMDRKLELDARFKEIRSKIKDLNLK
>Mature_277_residues
MVIVHSNEIARPIFKSQDQLFTLYQGDCNEVLPQFENQFDLIFADPPYFLSNDGLSIQSGKIVSVNKGDWDKEESVNDID
EFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRILQKLDFKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHK
HVFNYEVLKKINNDKQMRDVWSFPAIAPWEKANGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLERQF
IGIEKESEFVKISMDRKLELDARFKEIRSKIKDLNLK

Specific function: This methylase recognizes the double-stranded sequence GATC, causes specific methylation on A-2 on both strands, and protects the DNA from cleavage by the MboI endonuclease [H]

COG id: COG0863

COG function: function code L; DNA modification methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI87082238, Length=264, Percent_Identity=28.030303030303, Blast_Score=86, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002941
- InterPro:   IPR002052
- InterPro:   IPR001091 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 31898; Mature: 31898

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVIVHSNEIARPIFKSQDQLFTLYQGDCNEVLPQFENQFDLIFADPPYFLSNDGLSIQSG
CEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCEECCCCCEEECC
KIVSVNKGDWDKEESVNDIDEFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRILQKLD
EEEEECCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
FKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHVFNYEVLKKINNDKQMRDV
HHHHHHEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
WSFPAIAPWEKANGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLERQF
HCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IGIEKESEFVKISMDRKLELDARFKEIRSKIKDLNLK
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MVIVHSNEIARPIFKSQDQLFTLYQGDCNEVLPQFENQFDLIFADPPYFLSNDGLSIQSG
CEEEECCHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCEEEEECCCCCEECCCCCEEECC
KIVSVNKGDWDKEESVNDIDEFNYQWINNAKKALKNTGSLLISGTYHNIFSLGRILQKLD
EEEEECCCCCCCCCCCCHHHHHCHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHH
FKILNLITWQKTNPPPNFSCRYLTHSAEQIIWARKSHKHKHVFNYEVLKKINNDKQMRDV
HHHHHHEEECCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHHHHHHHHCCCHHHHHH
WSFPAIAPWEKANGKHPTQKPLALLVRLLLMASDENSLIGDPFSGSSTTGIAANLLERQF
HCCCCCCCCHHCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
IGIEKESEFVKISMDRKLELDARFKEIRSKIKDLNLK
CCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8506128 [H]