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The map label for this gene is rpoD [H]

Identifier: 188526895

GI number: 188526895

Start: 80054

End: 82039

Strand: Reverse

Name: rpoD [H]

Synonym: HPSH_00435

Alternate gene names: 188526895

Gene position: 82039-80054 (Counterclockwise)

Preceding gene: 188526896

Following gene: 188526894

Centisome position: 5.1

GC content: 38.97

Gene sequence:

>1986_bases
ATGAAAAAGAAAGTTAACGAAGAAAAAGCCCCAAAAAAAACCAAAACAGAAGCCACACAAGAAAATAAAATCAAAGAGAG
CAAAATTAAAGAAGCTAAAACCAAAGAAACTAAAGCGAAAGAACTTGTTCCTGTTAAAAAGCTTAGTTTCAATGAAGCGT
TAGAAGAATTGTTCGCTAATTCCTTAAGCGATTGCGTTTCTTATGAGTCTATCATTCAAATCAGCGCGAAAGTCCCCACT
CTAGCCCAAGTCAAAAAAATCAAAGAATTGTGCCAAAAATACCAAAAGAAATTAGTCAGCTCTTCAGAATACGCTAAAAA
ACTCAATGCGATTGACAAGATTAAAAAAACTGAAGAAAAGAAAAAAGTTTTAGATGAAGAATTAGAAGATGGCTATGACT
TTTTGAAAGAAAAGGATTTTTTAGAGTGGAGCAGAAGCGATAGCCCTGTGCGCATGTATTTGCGCGAGATGGGGGATATA
AAACTTTTAAGCAAAGATGAAGAGATTGAATTGAGCAAGCAAATCCGCTTGGGCGAAGACATTATTTTAGACGCGATTTG
CTCGGTGCCGTATTTGATTGATTTTATCTATGCGTATAAAGACGCTTTAATCAATCGTGAAAGAAGGGTTAAAGAGCTTT
TTAGGAGCTTTGATGATGACGATGAAAATAGCGTGAGCGATTCTAAAAAAGATGACGACAGCGAAGAGGATGAAGAAAAC
GAAGAAAGGAAAAAAGTCGTTTCTGAAAAAGACAGGAAGCGTGTAGAAAAGGTTCAAGAAAGCTTTAAAGCCCTAGATAA
GGCTAAAAAAGAATGGCTCAAAGCCCTTGAAGCCCCTGTAGATGAAAAAGAAGATGAATTGGTGCGTTTATTGACCCTAG
CTTACAAACGCCAAACGCTCAAAGACAGACTCTATGACTTAGAGCCTACTAGCAAACTGATTAATGAATTAGTCAAAACG
ATGGAAACCACTTTAAAAAGCGGCGATGGGTTTGAAAAAGAGTTGAAACGCTTGGAATACAAACTGCCCTTATTCAATGA
CACCTTGATCGCAAACCATAAAAAAATCCTTGCTAATATCACTAACATGACTAAAGAAGATATTATCGCTCAAGTCCCAG
AAGCGACTATGGTGAGCGTGTATATGGATCTCAAAAAGCTTTTTTTGACTAAAGAAGCGAGCGAAGAGGGCTTTGATTTA
GACCCTAACAAGCTAAAAGAAATTTTAGAGCAAATCAAAAGAGGGAAATTGATTTCCGATCGCGCTAAAAACAAAATGGC
TAAATCCAATTTAAGGTTGGTGGTGAGCATCGCTAAACGATTCACGAGCAGAGGCTTACCATTCTTGGATTTGATTCAAG
AGGGTAATATCGGCTTGATGAAAGCAGTGGATAAGTTTGAGCATGAAAAGGGCTTCAAGTTTTCTACCTATGCGACCTGG
TGGATCAAGCAAGCTATCAGCAGAGCCATAGCCGATCAAGCCCGCACTATCCGCATCCCCATTCACATGATTGATACGAT
TAATCGCATCAATAAAGTCATGCGCAAACACATTCAAGAAAACGGCAAAGAGCCTGATTTGGAAGTGGTGGCTGAAGAAG
TGGGGCTTTCGTTAGATAAAGTGAAGAATGTGATTAAGGTTACTAAAGAGCCTATCAGTTTGGAAACCCCAGTCGGCAAT
GATGATGACGGCAAATTTGGGGATTTCGTGGAAGATAAAAATATCGTTAGCTCCATTGATCACATCATGCGAGAAGATTT
GAAAGTGCAAATTGAAAGCGTTTTGGATCAGTTGAATGAGCGAGAAAAAGCGGTGATTCGCATGCGTTTTGGGCTTTTAG
ACGATGAGAGCGATCGCACTTTAGAAGAAATCGGCAAGGAATTGAATGTTACTAGAGAAAGGGTGCGCCAGATTGAAAGC
TCTGCGATTAAAAAATTGAGAAGCCCGCAATACGGGCGCATTTTAAGAAACTATTTGCGCATTTGA

Upstream 100 bases:

>100_bases
TTTAAGCTATTTTTTAGTAAAATAACAAAGGATTAGGTGTATCTTATCTTTATAGACTTGGCTTGCAAAAAGCATGGGTT
CTTATTTTATGGAGTGTCCA

Downstream 100 bases:

>100_bases
TGTAAGGTTTTTCTAAAGCATGCGTTATTTTCTTGTAGTTTTCTTGTTTTTGTTTGTGGGTTGCATGCAAAAGAATTTCA
CGCTCAAAGATTTATCCTTG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-70 [H]

Number of amino acids: Translated: 661; Mature: 661

Protein sequence:

>661_residues
MKKKVNEEKAPKKTKTEATQENKIKESKIKEAKTKETKAKELVPVKKLSFNEALEELFANSLSDCVSYESIIQISAKVPT
LAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEKKKVLDEELEDGYDFLKEKDFLEWSRSDSPVRMYLREMGDI
KLLSKDEEIELSKQIRLGEDIILDAICSVPYLIDFIYAYKDALINRERRVKELFRSFDDDDENSVSDSKKDDDSEEDEEN
EERKKVVSEKDRKRVEKVQESFKALDKAKKEWLKALEAPVDEKEDELVRLLTLAYKRQTLKDRLYDLEPTSKLINELVKT
METTLKSGDGFEKELKRLEYKLPLFNDTLIANHKKILANITNMTKEDIIAQVPEATMVSVYMDLKKLFLTKEASEEGFDL
DPNKLKEILEQIKRGKLISDRAKNKMAKSNLRLVVSIAKRFTSRGLPFLDLIQEGNIGLMKAVDKFEHEKGFKFSTYATW
WIKQAISRAIADQARTIRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDKVKNVIKVTKEPISLETPVGN
DDDGKFGDFVEDKNIVSSIDHIMREDLKVQIESVLDQLNEREKAVIRMRFGLLDDESDRTLEEIGKELNVTRERVRQIES
SAIKKLRSPQYGRILRNYLRI

Sequences:

>Translated_661_residues
MKKKVNEEKAPKKTKTEATQENKIKESKIKEAKTKETKAKELVPVKKLSFNEALEELFANSLSDCVSYESIIQISAKVPT
LAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEKKKVLDEELEDGYDFLKEKDFLEWSRSDSPVRMYLREMGDI
KLLSKDEEIELSKQIRLGEDIILDAICSVPYLIDFIYAYKDALINRERRVKELFRSFDDDDENSVSDSKKDDDSEEDEEN
EERKKVVSEKDRKRVEKVQESFKALDKAKKEWLKALEAPVDEKEDELVRLLTLAYKRQTLKDRLYDLEPTSKLINELVKT
METTLKSGDGFEKELKRLEYKLPLFNDTLIANHKKILANITNMTKEDIIAQVPEATMVSVYMDLKKLFLTKEASEEGFDL
DPNKLKEILEQIKRGKLISDRAKNKMAKSNLRLVVSIAKRFTSRGLPFLDLIQEGNIGLMKAVDKFEHEKGFKFSTYATW
WIKQAISRAIADQARTIRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDKVKNVIKVTKEPISLETPVGN
DDDGKFGDFVEDKNIVSSIDHIMREDLKVQIESVLDQLNEREKAVIRMRFGLLDDESDRTLEEIGKELNVTRERVRQIES
SAIKKLRSPQYGRILRNYLRI
>Mature_661_residues
MKKKVNEEKAPKKTKTEATQENKIKESKIKEAKTKETKAKELVPVKKLSFNEALEELFANSLSDCVSYESIIQISAKVPT
LAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEKKKVLDEELEDGYDFLKEKDFLEWSRSDSPVRMYLREMGDI
KLLSKDEEIELSKQIRLGEDIILDAICSVPYLIDFIYAYKDALINRERRVKELFRSFDDDDENSVSDSKKDDDSEEDEEN
EERKKVVSEKDRKRVEKVQESFKALDKAKKEWLKALEAPVDEKEDELVRLLTLAYKRQTLKDRLYDLEPTSKLINELVKT
METTLKSGDGFEKELKRLEYKLPLFNDTLIANHKKILANITNMTKEDIIAQVPEATMVSVYMDLKKLFLTKEASEEGFDL
DPNKLKEILEQIKRGKLISDRAKNKMAKSNLRLVVSIAKRFTSRGLPFLDLIQEGNIGLMKAVDKFEHEKGFKFSTYATW
WIKQAISRAIADQARTIRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDKVKNVIKVTKEPISLETPVGN
DDDGKFGDFVEDKNIVSSIDHIMREDLKVQIESVLDQLNEREKAVIRMRFGLLDDESDRTLEEIGKELNVTRERVRQIES
SAIKKLRSPQYGRILRNYLRI

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=265, Percent_Identity=54.3396226415094, Blast_Score=306, Evalue=2e-84,
Organism=Escherichia coli, GI1789098, Length=234, Percent_Identity=43.1623931623932, Blast_Score=197, Evalue=2e-51,
Organism=Escherichia coli, GI1789871, Length=237, Percent_Identity=32.0675105485232, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1788231, Length=201, Percent_Identity=26.3681592039801, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 76698; Mature: 76698

Theoretical pI: Translated: 7.99; Mature: 7.99

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKKVNEEKAPKKTKTEATQENKIKESKIKEAKTKETKAKELVPVKKLSFNEALEELFAN
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLSDCVSYESIIQISAKVPTLAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KKVLDEELEDGYDFLKEKDFLEWSRSDSPVRMYLREMGDIKLLSKDEEIELSKQIRLGED
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHCCHH
IILDAICSVPYLIDFIYAYKDALINRERRVKELFRSFDDDDENSVSDSKKDDDSEEDEEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
EERKKVVSEKDRKRVEKVQESFKALDKAKKEWLKALEAPVDEKEDELVRLLTLAYKRQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KDRLYDLEPTSKLINELVKTMETTLKSGDGFEKELKRLEYKLPLFNDTLIANHKKILANI
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TNMTKEDIIAQVPEATMVSVYMDLKKLFLTKEASEEGFDLDPNKLKEILEQIKRGKLISD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
RAKNKMAKSNLRLVVSIAKRFTSRGLPFLDLIQEGNIGLMKAVDKFEHEKGFKFSTYATW
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHH
WIKQAISRAIADQARTIRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDK
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHH
VKNVIKVTKEPISLETPVGNDDDGKFGDFVEDKNIVSSIDHIMREDLKVQIESVLDQLNE
HHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKAVIRMRFGLLDDESDRTLEEIGKELNVTRERVRQIESSAIKKLRSPQYGRILRNYLR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
I
C
>Mature Secondary Structure
MKKKVNEEKAPKKTKTEATQENKIKESKIKEAKTKETKAKELVPVKKLSFNEALEELFAN
CCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
SLSDCVSYESIIQISAKVPTLAQVKKIKELCQKYQKKLVSSSEYAKKLNAIDKIKKTEEK
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHH
KKVLDEELEDGYDFLKEKDFLEWSRSDSPVRMYLREMGDIKLLSKDEEIELSKQIRLGED
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEEEECCCCHHHHHHHHHCCHH
IILDAICSVPYLIDFIYAYKDALINRERRVKELFRSFDDDDENSVSDSKKDDDSEEDEEN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHH
EERKKVVSEKDRKRVEKVQESFKALDKAKKEWLKALEAPVDEKEDELVRLLTLAYKRQTL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
KDRLYDLEPTSKLINELVKTMETTLKSGDGFEKELKRLEYKLPLFNDTLIANHKKILANI
HHHHCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
TNMTKEDIIAQVPEATMVSVYMDLKKLFLTKEASEEGFDLDPNKLKEILEQIKRGKLISD
HHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCHHH
RAKNKMAKSNLRLVVSIAKRFTSRGLPFLDLIQEGNIGLMKAVDKFEHEKGFKFSTYATW
HHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHH
WIKQAISRAIADQARTIRIPIHMIDTINRINKVMRKHIQENGKEPDLEVVAEEVGLSLDK
HHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCHHH
VKNVIKVTKEPISLETPVGNDDDGKFGDFVEDKNIVSSIDHIMREDLKVQIESVLDQLNE
HHHHHHHHCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
REKAVIRMRFGLLDDESDRTLEEIGKELNVTRERVRQIESSAIKKLRSPQYGRILRNYLR
HHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
I
C

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]