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The map label for this gene is eptA [H]

Identifier: 188526833

GI number: 188526833

Start: 20893

End: 22464

Strand: Reverse

Name: eptA [H]

Synonym: HPSH_00115

Alternate gene names: 188526833

Gene position: 22464-20893 (Counterclockwise)

Preceding gene: 188526834

Following gene: 188526832

Centisome position: 1.4

GC content: 38.68

Gene sequence:

>1572_bases
TTGGCATCATTATTCCATTTGAAGTTTTTAAAGCCCTTAAGTTGTCTGCAAGCCGGTTTGCTTTATAACCTTATTTTTGG
CGTTTTATACCATTTCCCTTTGTTCGCTTATGTCTATAAAGAAAGCAACCAGGTTAGTTTTATAGCCATGATTGTTGTTG
TGCTTTTTTGCGTTAATGGCGCTCTTTTTTTAACATTAGGCTTGATCTCTATCCATTTGATGCGCCTAAGCGCGATTGTT
TTTAGTTTGCTCAATTCCATCGCTTTCTATTTCATTAGCGCTTATAAGGTGTTTTTAAATAAGAGCATGATGGGTAATGT
CTTAAACACCAATACGCATGAGCTTTTAGGCTTTTTGAGCGTTAAATTATTCATTGTTATCGTTGTTTTTGGGGTGTTGC
CTGGCTATGTCATCTATAAAATCCCCCTCAAAAATTCTTCTAAAAAAGCGCCCTTTTTAGCGATCTTGGCGTTAGTGTTT
ATCTTTATCGCTAGCGCTTTAATGAACGCTAAAAATTGGCTGTGGTTTGACAAGCATGCGAAATTCATAGGGGGCTTAAT
TTTGCCTTTCGCTTATAGCGTGAACGCTTTTAGAGTGAGCGCTCTTAAATTTTTCGCCCCCACTATCAAGCCGCTCCCTC
TTTTTTCGCCCAATCGTTCTCATTCGTTTGTGGTGCTAGTCATTGGCGAAAGCGCTAGGAAGCATAATTACGCCCTTTAT
GGCTATAAAAAACCCACCACCCCAAAATTAAGCAAGCGTTTAGCCGATAATGAACTCACTCTTTTTAACGCCACTTCTTG
CGCCACTTACACGACCGCGAGTTTGGAATGCATTTTAGATTCTTCTTTTAAAAACAGCTTGAACGCTTATGAAAATTTAC
CGACTTACTTGACTAAAGCCGGTATCAAAGTCTTTTGGTATAGCGCGAATGACGGCGAAAAGAATGTTAAGGTTACAAGC
TATCTTAAAAACTATGAATTGATTCAAAAATGCCCCAATTGTGAAGCGATCGCTCCTTATGATGAATCCTTACTCTATAA
TTTGCCTGACCTTTTAAAAGAACACTCTAATGAAAATGTCTTACTCATCTTACACCTTGCAGGCTCGCATGGTCCCAACT
ATGACAATAAAGTGCCTTTAAATTTTAGGGTGTTTAAACCCTATTGCTCAAGCGCTGATTTGTCTTCTTGCTCCAAAGAA
AGCTTGATTAACGCCTATGACAACACCATTTTTTACAACGACTATCTGTTAGACAAGATCATTAGCATGCTCAAAAACGC
CAAGCAGCCCGCCTTAATGATCTATTTGAGCGATCATGGCGAAAGTTTGGGCGAAGGAGCGTATTATTTGCATGGCATTC
CTAAAAGCATCGCCCCCAAAGAACAATACGAGGTCCCCTTTATTGTTTATGCCAATGATCTTTTTAAAGAGAAGCATTCC
ATCATTCAAACCCAAACCCCAATCAATCAAAATGTTATTTTCCATAGCATTTTAGGAGTGTTTGAAGATTTTAAAAACCC
TAGCGCTGTTTATCGCCCTTCTTTAGATCTGCTTAAACACAAAAAAGAATAA

Upstream 100 bases:

>100_bases
ATCTTGCGACCTAAAAGAGGGAGTTTTTCGCGCTGAATAAACCATAAAATCCCCATACCTACGATATAATCCTAAGATTG
ATTGATTCTAAGGGGCGTGT

Downstream 100 bases:

>100_bases
AATAATACGCATGAAAAGATTCTTATTTAAACAAAAATTTTTGGAAAGCCTGCCTAAAAGCTTTTCTAAAACTTTATTAG
CGCTCAGTTTGGGCTTGATC

Product: lipid A phosphoethanolamine transferase

Products: NA

Alternate protein names: Polymyxin resistance protein pmrC [H]

Number of amino acids: Translated: 523; Mature: 522

Protein sequence:

>523_residues
MASLFHLKFLKPLSCLQAGLLYNLIFGVLYHFPLFAYVYKESNQVSFIAMIVVVLFCVNGALFLTLGLISIHLMRLSAIV
FSLLNSIAFYFISAYKVFLNKSMMGNVLNTNTHELLGFLSVKLFIVIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASALMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNRSHSFVVLVIGESARKHNYALY
GYKKPTTPKLSKRLADNELTLFNATSCATYTTASLECILDSSFKNSLNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTS
YLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKE
SLINAYDNTIFYNDYLLDKIISMLKNAKQPALMIYLSDHGESLGEGAYYLHGIPKSIAPKEQYEVPFIVYANDLFKEKHS
IIQTQTPINQNVIFHSILGVFEDFKNPSAVYRPSLDLLKHKKE

Sequences:

>Translated_523_residues
MASLFHLKFLKPLSCLQAGLLYNLIFGVLYHFPLFAYVYKESNQVSFIAMIVVVLFCVNGALFLTLGLISIHLMRLSAIV
FSLLNSIAFYFISAYKVFLNKSMMGNVLNTNTHELLGFLSVKLFIVIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVF
IFIASALMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNRSHSFVVLVIGESARKHNYALY
GYKKPTTPKLSKRLADNELTLFNATSCATYTTASLECILDSSFKNSLNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTS
YLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKE
SLINAYDNTIFYNDYLLDKIISMLKNAKQPALMIYLSDHGESLGEGAYYLHGIPKSIAPKEQYEVPFIVYANDLFKEKHS
IIQTQTPINQNVIFHSILGVFEDFKNPSAVYRPSLDLLKHKKE
>Mature_522_residues
ASLFHLKFLKPLSCLQAGLLYNLIFGVLYHFPLFAYVYKESNQVSFIAMIVVVLFCVNGALFLTLGLISIHLMRLSAIVF
SLLNSIAFYFISAYKVFLNKSMMGNVLNTNTHELLGFLSVKLFIVIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFI
FIASALMNAKNWLWFDKHAKFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNRSHSFVVLVIGESARKHNYALYG
YKKPTTPKLSKRLADNELTLFNATSCATYTTASLECILDSSFKNSLNAYENLPTYLTKAGIKVFWYSANDGEKNVKVTSY
LKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENVLLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKES
LINAYDNTIFYNDYLLDKIISMLKNAKQPALMIYLSDHGESLGEGAYYLHGIPKSIAPKEQYEVPFIVYANDLFKEKHSI
IQTQTPINQNVIFHSILGVFEDFKNPSAVYRPSLDLLKHKKE

Specific function: Catalyzes the addition of a phosphoethanolamine moiety to the lipid A. The phosphoethanolamine modification is required for resistance to polymyxin [H]

COG id: COG2194

COG function: function code R; Predicted membrane-associated, metal-dependent hydrolase

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoethanolamine transferase family. EptA subfamily [H]

Homologues:

Organism=Escherichia coli, GI87082372, Length=488, Percent_Identity=28.6885245901639, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI87082286, Length=453, Percent_Identity=26.0485651214128, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI87082223, Length=428, Percent_Identity=26.8691588785047, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790392, Length=378, Percent_Identity=24.8677248677249, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1787035, Length=277, Percent_Identity=26.3537906137184, Blast_Score=80, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR017850
- InterPro:   IPR012549
- InterPro:   IPR000917 [H]

Pfam domain/function: PF08019 DUF1705; PF00884 Sulfatase [H]

EC number: NA

Molecular weight: Translated: 59010; Mature: 58878

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS01037 SBP_BACTERIAL_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLFHLKFLKPLSCLQAGLLYNLIFGVLYHFPLFAYVYKESNQVSFIAMIVVVLFCVNG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
ALFLTLGLISIHLMRLSAIVFSLLNSIAFYFISAYKVFLNKSMMGNVLNTNTHELLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFIVIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASALMNAKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNRSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYKKPTTPKLSKRLADNELTLFNATSCATYTTASLECILDSSFKNSLNAYENLPTYLTKA
ECCCCCCHHHHHHHCCCCEEEEECCCCCEEEHHHHHEEECCHHHHHHHHHHCCHHHHHHC
GIKVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCE
LLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESLINAYDNTIFYNDYLLDKI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCEEEEHHHHHHHH
ISMLKNAKQPALMIYLSDHGESLGEGAYYLHGIPKSIAPKEQYEVPFIVYANDLFKEKHS
HHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHCCCCEEEECHHHHHHHHH
IIQTQTPINQNVIFHSILGVFEDFKNPSAVYRPSLDLLKHKKE
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCC
>Mature Secondary Structure 
ASLFHLKFLKPLSCLQAGLLYNLIFGVLYHFPLFAYVYKESNQVSFIAMIVVVLFCVNG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCC
ALFLTLGLISIHLMRLSAIVFSLLNSIAFYFISAYKVFLNKSMMGNVLNTNTHELLGFLS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
VKLFIVIVVFGVLPGYVIYKIPLKNSSKKAPFLAILALVFIFIASALMNAKNWLWFDKHA
HHHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEHHHH
KFIGGLILPFAYSVNAFRVSALKFFAPTIKPLPLFSPNRSHSFVVLVIGESARKHNYALY
HHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCEEEE
GYKKPTTPKLSKRLADNELTLFNATSCATYTTASLECILDSSFKNSLNAYENLPTYLTKA
ECCCCCCHHHHHHHCCCCEEEEECCCCCEEEHHHHHEEECCHHHHHHHHHHCCHHHHHHC
GIKVFWYSANDGEKNVKVTSYLKNYELIQKCPNCEAIAPYDESLLYNLPDLLKEHSNENV
CCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCHHHHHCCHHHHHHCCCCCE
LLILHLAGSHGPNYDNKVPLNFRVFKPYCSSADLSSCSKESLINAYDNTIFYNDYLLDKI
EEEEEECCCCCCCCCCCCCCEEEEECCCCCCCCHHHCCHHHHHHHHCCEEEEHHHHHHHH
ISMLKNAKQPALMIYLSDHGESLGEGAYYLHGIPKSIAPKEQYEVPFIVYANDLFKEKHS
HHHHHCCCCCEEEEEEECCCCCCCCCCEEEECCCCCCCCCHHCCCCEEEECHHHHHHHHH
IIQTQTPINQNVIFHSILGVFEDFKNPSAVYRPSLDLLKHKKE
HHHCCCCCCCHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8391535; 11677609 [H]