BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is livF [H]

Identifier: 187930602

GI number: 187930602

Start: 3706538

End: 3707278

Strand: Reverse

Name: livF [H]

Synonym: Rpic_3538

Alternate gene names: 187930602

Gene position: 3707278-3706538 (Counterclockwise)

Preceding gene: 187930603

Following gene: 187930601

Centisome position: 94.03

GC content: 65.45

Gene sequence:

>741_bases
ATGAGCACGCCCGCGCTCCAGATCAAGGACCTGCACGCCTGGTACGGCGAATCGCACATCCTGCATGGTGTCGACCTGAC
CGTGAACCGCGGCGAGGTGGTCACGCTGCTCGGCCGCAACGGTGCGGGCCGTACCACCACGCTGCGCGCCATCATGGGCC
TGGTGGGCACGCGCAAAGGCTCCATCGTCGTGCATGACGCTGGCGGCCAGGGTGTGGAAACGATCGACCTGCCCACGCAC
AAGATCGCCCACTGCGGCATCGGGTACTGCCCCGAGGAGCGCGGCATTTTCTCGAGCCTGTCGTGCGAAGAGAACCTGAT
GCTGCCGCCGGTGCTGAAGGGCCAGGTGGCCAAGACCGGCAATGCCGGTATGAGCATCGACGAGATCTACGAGATGTTCC
CCAACCTGGCCGAGCGCCGCCAAAGCCAGGGCACGCGCCTGTCGGGCGGCGAGCAGCAGATGCTGGCGGTCGGGCGCATC
CTGCGCACCGGCGCGAACCTGTTGCTGCTCGACGAGATTTCCGAAGGACTGGCGCCGGTGATCGTGCAGGCGCTGGCCCG
CATGATCCTGATGCTCAAGAAGCGCGGCTACACGGTGGTGATGGTGGAGCAGAACTTCCGCTTTGCCGCGCCGCTGGCAG
ACCGGTTTTATGTGATGGAACACGGCCGCATCGTCGAGCGTTTCGAGGCGGCGCAGTTGCAGGACAAGATGCCGGTGCTG
CACGAGCTGCTCGGCGTTTAA

Upstream 100 bases:

>100_bases
CATTGAGGCCACTGATCGACGCTGATCGATCACGCGACGCGGCCACACGCCGCGCATCAAACGTAAGCGAACGCTAGACC
CGAACACACGAGAACACTGC

Downstream 100 bases:

>100_bases
GAGGTTTTCCCGATCGACTTGCAAGACCCATGCGCCAGCGTGATCCGCGGCGCGCACGACACAGGAGACAAGACCATGAC
ACTCAAGAAGCTGGCTGGTG

Product: ABC transporter-like protein

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: LIV-I protein F [H]

Number of amino acids: Translated: 246; Mature: 245

Protein sequence:

>246_residues
MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDAGGQGVETIDLPTH
KIAHCGIGYCPEERGIFSSLSCEENLMLPPVLKGQVAKTGNAGMSIDEIYEMFPNLAERRQSQGTRLSGGEQQMLAVGRI
LRTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPVL
HELLGV

Sequences:

>Translated_246_residues
MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDAGGQGVETIDLPTH
KIAHCGIGYCPEERGIFSSLSCEENLMLPPVLKGQVAKTGNAGMSIDEIYEMFPNLAERRQSQGTRLSGGEQQMLAVGRI
LRTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPVL
HELLGV
>Mature_245_residues
STPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKGSIVVHDAGGQGVETIDLPTHK
IAHCGIGYCPEERGIFSSLSCEENLMLPPVLKGQVAKTGNAGMSIDEIYEMFPNLAERRQSQGTRLSGGEQQMLAVGRIL
RTGANLLLLDEISEGLAPVIVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPVLH
ELLGV

Specific function: Component of the leucine-specific transport system [H]

COG id: COG0410

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI31657092, Length=224, Percent_Identity=25.8928571428571, Blast_Score=83, Evalue=2e-16,
Organism=Homo sapiens, GI105990541, Length=201, Percent_Identity=27.363184079602, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI45446740, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI47078218, Length=204, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI21536376, Length=204, Percent_Identity=25.9803921568627, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI30795238, Length=214, Percent_Identity=28.9719626168224, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI27881501, Length=214, Percent_Identity=28.9719626168224, Blast_Score=75, Evalue=4e-14,
Organism=Homo sapiens, GI150417984, Length=203, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI116734710, Length=203, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI42741659, Length=248, Percent_Identity=26.6129032258064, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI9961252, Length=250, Percent_Identity=27.2, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI9961250, Length=250, Percent_Identity=27.2, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI4505771, Length=250, Percent_Identity=27.2, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI27436953, Length=203, Percent_Identity=27.0935960591133, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI153792144, Length=219, Percent_Identity=27.3972602739726, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI27477115, Length=203, Percent_Identity=25.615763546798, Blast_Score=65, Evalue=4e-11,
Organism=Escherichia coli, GI87082268, Length=225, Percent_Identity=42.2222222222222, Blast_Score=159, Evalue=1e-40,
Organism=Escherichia coli, GI1789593, Length=223, Percent_Identity=34.0807174887892, Blast_Score=111, Evalue=4e-26,
Organism=Escherichia coli, GI1789672, Length=223, Percent_Identity=28.2511210762332, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1788225, Length=234, Percent_Identity=31.6239316239316, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787029, Length=222, Percent_Identity=28.8288288288288, Blast_Score=94, Evalue=7e-21,
Organism=Escherichia coli, GI1789864, Length=263, Percent_Identity=25.0950570342205, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788897, Length=219, Percent_Identity=29.6803652968037, Blast_Score=85, Evalue=4e-18,
Organism=Escherichia coli, GI1788644, Length=242, Percent_Identity=28.9256198347107, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI1790525, Length=235, Percent_Identity=27.2340425531915, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1787089, Length=236, Percent_Identity=26.6949152542373, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI87081791, Length=229, Percent_Identity=27.9475982532751, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788450, Length=219, Percent_Identity=30.5936073059361, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1788761, Length=225, Percent_Identity=27.5555555555556, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790467, Length=223, Percent_Identity=27.3542600896861, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787370, Length=225, Percent_Identity=27.5555555555556, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787792, Length=223, Percent_Identity=27.3542600896861, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1786253, Length=249, Percent_Identity=28.5140562248996, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1787105, Length=228, Percent_Identity=27.6315789473684, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790190, Length=232, Percent_Identity=25, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI48995001, Length=230, Percent_Identity=25.6521739130435, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1786872, Length=224, Percent_Identity=28.125, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1786703, Length=212, Percent_Identity=28.3018867924528, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1787712, Length=226, Percent_Identity=26.9911504424779, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787112, Length=243, Percent_Identity=28.8065843621399, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1786398, Length=208, Percent_Identity=24.5192307692308, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI48994943, Length=235, Percent_Identity=24.2553191489362, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1788210, Length=255, Percent_Identity=23.921568627451, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789991, Length=231, Percent_Identity=29.004329004329, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI87081782, Length=238, Percent_Identity=25.6302521008403, Blast_Score=65, Evalue=3e-12,
Organism=Escherichia coli, GI1786319, Length=232, Percent_Identity=27.1551724137931, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17510237, Length=207, Percent_Identity=30.4347826086957, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI115533608, Length=224, Percent_Identity=29.9107142857143, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI71983516, Length=209, Percent_Identity=27.7511961722488, Blast_Score=74, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71983523, Length=209, Percent_Identity=27.7511961722488, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI71983510, Length=209, Percent_Identity=27.7511961722488, Blast_Score=74, Evalue=6e-14,
Organism=Caenorhabditis elegans, GI17565586, Length=223, Percent_Identity=26.9058295964126, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI25146777, Length=211, Percent_Identity=26.0663507109005, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI193202349, Length=243, Percent_Identity=23.8683127572016, Blast_Score=64, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323959, Length=221, Percent_Identity=24.4343891402715, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648314, Length=220, Percent_Identity=26.3636363636364, Blast_Score=84, Evalue=7e-17,
Organism=Drosophila melanogaster, GI24666092, Length=219, Percent_Identity=28.310502283105, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI28574150, Length=208, Percent_Identity=28.8461538461538, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI116007328, Length=208, Percent_Identity=28.8461538461538, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI221512771, Length=220, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24643674, Length=220, Percent_Identity=25, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24650853, Length=232, Percent_Identity=25.8620689655172, Blast_Score=64, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24650855, Length=232, Percent_Identity=25.8620689655172, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI85725262, Length=210, Percent_Identity=25.7142857142857, Blast_Score=64, Evalue=8e-11,
Organism=Drosophila melanogaster, GI281362751, Length=213, Percent_Identity=26.2910798122066, Blast_Score=64, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 26912; Mature: 26781

Theoretical pI: Translated: 7.25; Mature: 7.25

Prosite motif: PS00211 ABC_TRANSPORTER_1 ; PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKG
CCCCCCCHHHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC
SIVVHDAGGQGVETIDLPTHKIAHCGIGYCPEERGIFSSLSCEENLMLPPVLKGQVAKTG
CEEEECCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAGMSIDEIYEMFPNLAERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPV
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHH
IVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPVL
HHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEHHCCHHHHHHHHHHHHHCHHHH
HELLGV
HHHHCC
>Mature Secondary Structure 
STPALQIKDLHAWYGESHILHGVDLTVNRGEVVTLLGRNGAGRTTTLRAIMGLVGTRKG
CCCCCCHHHHHHHHCCCEEEECEEEEECCCCEEEEEECCCCCCHHHHHHHHHHHCCCCC
SIVVHDAGGQGVETIDLPTHKIAHCGIGYCPEERGIFSSLSCEENLMLPPVLKGQVAKTG
CEEEECCCCCCCEEEECCCHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
NAGMSIDEIYEMFPNLAERRQSQGTRLSGGEQQMLAVGRILRTGANLLLLDEISEGLAPV
CCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEHHHHCCHHHH
IVQALARMILMLKKRGYTVVMVEQNFRFAAPLADRFYVMEHGRIVERFEAAQLQDKMPVL
HHHHHHHHHHHHHCCCCEEEEEECCCEEECCHHHHEEEHHCCHHHHHHHHHHHHHCHHHH
HELLGV
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2195019; 8041620; 9278503 [H]