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The map label for this gene is kefF [H]

Identifier: 187930446

GI number: 187930446

Start: 3543394

End: 3543957

Strand: Reverse

Name: kefF [H]

Synonym: Rpic_3380

Alternate gene names: 187930446

Gene position: 3543957-3543394 (Counterclockwise)

Preceding gene: 187930452

Following gene: 187930445

Centisome position: 89.89

GC content: 67.02

Gene sequence:

>564_bases
ATGACACCCTCGCGCATTCTTGTGATCTACGCGCATCCTGCGCCGCGCCGGTCGCGCGCCAACAAGCCACTCGCGCGTGC
GCTGGCCAGCTTGCCCGGCGTGGCCATGCACGACCTGTACTGGCATTACCCCGATTTCGACATCGACGTGCGTGCCGAGC
AGGAAGCGGTGGAAGCGGCGGAGCTGATCGTCTTCCAGTTTCCGGTGCAGTGGTATGCCACGCCGTCGCTGCTCAAGGAG
TGGATCGACGTGGTGCTGGAGATGGGCTGGGCCTATGGGCCGGGCGGCACGGCGTTGCGCGGCAAGCATCTAATGGTGCT
CGCCACCACTGGCGGCCGCGCGCATTCCTACAACCCGGATGGCGTTCACGGCCACACGTTCGACATGTTCCTGCTGCCGC
TGCAGCAGACCGCCGCGCTGTGCGGCATGCAATGGCAGCCGCCGCATGTGCTGCACGACGCCGACGATGCGTCTGCTGAC
GTCATCGACGCCCATATCGCGCAGGTCCGCGCCGCACTGCAGCCCTGGCTCCGGGCCGATGCCGACGCCAACTCCGTCGC
CTGA

Upstream 100 bases:

>100_bases
CGTTACATCACTACCCGCAGTACGATCGCCGCCTGTAACGCCGCTCAACAATAGCCGTGCGAAGATGCGCCATCAAGACG
CCCCAGCCTCCTGATTTTCC

Downstream 100 bases:

>100_bases
CGTTTCCGATCTCTTCCCTGCCTATGCAATCGCACGATCTGCTCGTCAATTTCGTCATCTACCTGGCTGCGGCTGTGGCA
GCGGTGCCGCTGGCGCGTCG

Product: NAD(P)H dehydrogenase (quinone)

Products: NAD+; NADP+; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MTPSRILVIYAHPAPRRSRANKPLARALASLPGVAMHDLYWHYPDFDIDVRAEQEAVEAAELIVFQFPVQWYATPSLLKE
WIDVVLEMGWAYGPGGTALRGKHLMVLATTGGRAHSYNPDGVHGHTFDMFLLPLQQTAALCGMQWQPPHVLHDADDASAD
VIDAHIAQVRAALQPWLRADADANSVA

Sequences:

>Translated_187_residues
MTPSRILVIYAHPAPRRSRANKPLARALASLPGVAMHDLYWHYPDFDIDVRAEQEAVEAAELIVFQFPVQWYATPSLLKE
WIDVVLEMGWAYGPGGTALRGKHLMVLATTGGRAHSYNPDGVHGHTFDMFLLPLQQTAALCGMQWQPPHVLHDADDASAD
VIDAHIAQVRAALQPWLRADADANSVA
>Mature_186_residues
TPSRILVIYAHPAPRRSRANKPLARALASLPGVAMHDLYWHYPDFDIDVRAEQEAVEAAELIVFQFPVQWYATPSLLKEW
IDVVLEMGWAYGPGGTALRGKHLMVLATTGGRAHSYNPDGVHGHTFDMFLLPLQQTAALCGMQWQPPHVLHDADDASADV
IDAHIAQVRAALQPWLRADADANSVA

Specific function: Required for full activity of kefC [H]

COG id: COG2249

COG function: function code R; Putative NADPH-quinone reductase (modulator of drug activity B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD(P)H dehydrogenase (quinone) family. KefF subfamily [H]

Homologues:

Organism=Homo sapiens, GI156564357, Length=147, Percent_Identity=35.3741496598639, Blast_Score=79, Evalue=2e-15,
Organism=Homo sapiens, GI4505415, Length=121, Percent_Identity=34.7107438016529, Blast_Score=74, Evalue=7e-14,
Organism=Escherichia coli, GI1786231, Length=174, Percent_Identity=47.1264367816092, Blast_Score=153, Evalue=5e-39,
Organism=Escherichia coli, GI1789750, Length=163, Percent_Identity=40.4907975460123, Blast_Score=130, Evalue=7e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003680 [H]

Pfam domain/function: PF02525 Flavodoxin_2 [H]

EC number: 1.6.99.2

Molecular weight: Translated: 20636; Mature: 20504

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSRILVIYAHPAPRRSRANKPLARALASLPGVAMHDLYWHYPDFDIDVRAEQEAVEAA
CCCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHEEECCCCCEEECCHHHHHHHH
ELIVFQFPVQWYATPSLLKEWIDVVLEMGWAYGPGGTALRGKHLMVLATTGGRAHSYNPD
HEEEEECCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
GVHGHTFDMFLLPLQQTAALCGMQWQPPHVLHDADDASADVIDAHIAQVRAALQPWLRAD
CCCCCCHHHEEECHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADANSVA
CCCCCCC
>Mature Secondary Structure 
TPSRILVIYAHPAPRRSRANKPLARALASLPGVAMHDLYWHYPDFDIDVRAEQEAVEAA
CCCEEEEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHEEECCCCCEEECCHHHHHHHH
ELIVFQFPVQWYATPSLLKEWIDVVLEMGWAYGPGGTALRGKHLMVLATTGGRAHSYNPD
HEEEEECCHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
GVHGHTFDMFLLPLQQTAALCGMQWQPPHVLHDADDASADVIDAHIAQVRAALQPWLRAD
CCCCCCHHHEEECHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCC
ADANSVA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; NADPH; H+; acceptor

Specific reaction: NAD(P)H + H+ + acceptor = NAD(P)+ + reduced acceptor

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA