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The map label for this gene is tuf1 [H]

Identifier: 187930388

GI number: 187930388

Start: 3469162

End: 3470352

Strand: Reverse

Name: tuf1 [H]

Synonym: Rpic_3321

Alternate gene names: 187930388

Gene position: 3470352-3469162 (Counterclockwise)

Preceding gene: 187930389

Following gene: 187930387

Centisome position: 88.02

GC content: 61.88

Gene sequence:

>1191_bases
ATGGCAAAGGAAAAGTTCGAGCGGACTAAGCCGCACGTGAACGTTGGTACGATTGGTCACGTTGACCACGGCAAGACGAC
GCTGACGGCAGCGATCACGACCGTGCTGGCGACCAAGTTTGGTGGTGCCGCCAAGGCATACGACCAGATTGATGCGGCGC
CGGAAGAAAAGGCACGCGGCATCACGATCAACACCGCACACGTTGAGTACGAAACGGCTAACCGTCACTACGCGCACGTT
GACTGCCCGGGCCACGCCGACTACGTCAAGAACATGATTACCGGTGCCGCCCAGATGGACGGCGCAATCCTGGTGTGCTC
GGCCGCTGACGGCCCGATGCCGCAAACGCGTGAGCACATCCTGCTGGCCCGCCAGGTTGGTGTGCCGTACATCGTCGTGT
TCCTGAACAAGTGCGACATGGTGGACGACGCTGAGCTGCTGGAGCTGGTCGAGATGGAGGTGCGCGAACTTCTGTCGAAG
TACGACTTCCCGGGCGACGACACCCCGATCATCAAGGGTTCGGCCAAGCTGGCGCTGGAAGGCGACAAGGGCGAGCTGGG
CGAAGTGGCCATCATGAACCTGGCCGACGCACTGGACACCTACATCCCGACGCCGGAGCGCGCTGTTGACGGCACGTTCC
TGATGCCGGTGGAAGACGTGTTCTCGATCTCGGGTCGCGGCACCGTGGTGACCGGCCGTATCGAGCGCGGCGTAGTCAAG
GTCGGCGAAGAGCTGGAAATCGTCGGTATCAAGGCCACGCAGAAGACCACCTGCACGGGCGTGGAAATGTTCCGCAAGCT
GCTGGACCAAGGTCAGGCAGGCGACAACGTCGGTATCCTGCTGCGCGGCACGAAGCGCGAAGACGTCGAGCGCGGCCAAG
TGCTATGCAAGCCGGGTTCGATCAAGCCGCACACGCACTTCACGGGCGAGGTCTACATCCTGTCGAAGGACGAAGGCGGC
CGTCACACGCCGTTCTTCAACAACTACCGTCCGCAGTTCTATTTCCGTACGACGGACGTGACCGGCTCGATCGAGCTGCC
GGCCGACAAGGAAATGGTCATGCCGGGCGACAACGTGTCGATCACCGTCAAGCTGATCGCCCCGATCGCCATGGAAGAAG
GTCTGCGCTTCGCTATCCGTGAAGGCGGTCGTACCGTCGGCGCCGGCGTCGTCGCTAAGATCATCGAGTAA

Upstream 100 bases:

>100_bases
TAGAGCACTCCCTTGGTAAGGGAGAGGTCGGCAGTTCGATCCTGCCCATGGGCACCACGCATCCACCAACGCAAATCGCT
ACCGAGACAGGAGTCGCGAC

Downstream 100 bases:

>100_bases
GTGCTGTAACTCTTCCGTGCGGCTGCCAACCGGCCGCACGTGAAGTGCCAGAGTGGGGTTGACCGAAATGGCAACCCCAA
AGCTGTTTTAGGGGTATAGC

Product: elongation factor Tu

Products: GDP; phosphate

Alternate protein names: EF-Tu 1 [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLATKFGGAAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVVK
VGEELEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLATKFGGAAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHV
DCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKCDMVDDAELLELVEMEVRELLSK
YDFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVVK
VGEELEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGG
RHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLATKFGGAAKAYDQIDAAPEEKARGITINTAHVEYETANRHYAHVD
CPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHILLARQVGVPYIVVFLNKCDMVDDAELLELVEMEVRELLSKY
DFPGDDTPIIKGSAKLALEGDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVVKV
GEELEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGSIKPHTHFTGEVYILSKDEGGR
HTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVSITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=57.8680203045685, Blast_Score=484, Evalue=1e-137,
Organism=Homo sapiens, GI223555963, Length=437, Percent_Identity=30.6636155606407, Blast_Score=161, Evalue=1e-39,
Organism=Homo sapiens, GI5729864, Length=437, Percent_Identity=30.6636155606407, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI4503475, Length=448, Percent_Identity=27.6785714285714, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI4503471, Length=442, Percent_Identity=28.7330316742081, Blast_Score=146, Evalue=3e-35,
Organism=Homo sapiens, GI46094014, Length=444, Percent_Identity=27.7027027027027, Blast_Score=122, Evalue=7e-28,
Organism=Homo sapiens, GI194018522, Length=434, Percent_Identity=28.3410138248848, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194018520, Length=434, Percent_Identity=28.3410138248848, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI194097354, Length=434, Percent_Identity=28.3410138248848, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI54607086, Length=319, Percent_Identity=29.7805642633229, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI4503507, Length=268, Percent_Identity=27.9850746268657, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI94966754, Length=135, Percent_Identity=31.8518518518519, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI53729339, Length=238, Percent_Identity=31.5126050420168, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI53729337, Length=238, Percent_Identity=31.5126050420168, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI157426893, Length=309, Percent_Identity=27.831715210356, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=81.2658227848101, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=81.2658227848101, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=341, Percent_Identity=30.791788856305, Blast_Score=135, Evalue=4e-33,
Organism=Escherichia coli, GI48994988, Length=275, Percent_Identity=31.6363636363636, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI1789108, Length=96, Percent_Identity=38.5416666666667, Blast_Score=79, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556456, Length=400, Percent_Identity=52.75, Blast_Score=410, Evalue=1e-115,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.2196382428941, Blast_Score=332, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI17552884, Length=456, Percent_Identity=29.6052631578947, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=456, Percent_Identity=29.6052631578947, Blast_Score=149, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI17506081, Length=295, Percent_Identity=30.5084745762712, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=28.1464530892449, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI115532067, Length=439, Percent_Identity=26.1958997722096, Blast_Score=121, Evalue=8e-28,
Organism=Caenorhabditis elegans, GI115532065, Length=439, Percent_Identity=26.1958997722096, Blast_Score=120, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI32566629, Length=355, Percent_Identity=28.7323943661972, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI32566301, Length=154, Percent_Identity=38.3116883116883, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17509919, Length=296, Percent_Identity=25.3378378378378, Blast_Score=83, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17557151, Length=237, Percent_Identity=26.5822784810127, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.6666666666667, Blast_Score=536, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6325337, Length=444, Percent_Identity=29.0540540540541, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6319594, Length=444, Percent_Identity=29.0540540540541, Blast_Score=145, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6320377, Length=336, Percent_Identity=30.0595238095238, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6322937, Length=333, Percent_Identity=25.5255255255255, Blast_Score=85, Evalue=2e-17,
Organism=Saccharomyces cerevisiae, GI6320863, Length=265, Percent_Identity=27.1698113207547, Blast_Score=84, Evalue=3e-17,
Organism=Saccharomyces cerevisiae, GI6323098, Length=192, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=59.2875318066158, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=59.2875318066158, Blast_Score=490, Evalue=1e-139,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.8134715025907, Blast_Score=412, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24652838, Length=455, Percent_Identity=29.010989010989, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI17137572, Length=455, Percent_Identity=29.010989010989, Blast_Score=151, Evalue=9e-37,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=28.7912087912088, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=28.7912087912088, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=28.7912087912088, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=28.7912087912088, Blast_Score=149, Evalue=3e-36,
Organism=Drosophila melanogaster, GI17137380, Length=348, Percent_Identity=31.0344827586207, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=118, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45550900, Length=355, Percent_Identity=29.0140845070423, Blast_Score=115, Evalue=4e-26,
Organism=Drosophila melanogaster, GI24647046, Length=269, Percent_Identity=28.2527881040892, Blast_Score=88, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24647048, Length=269, Percent_Identity=28.2527881040892, Blast_Score=88, Evalue=1e-17,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: 3.6.5.3

Molecular weight: Translated: 42981; Mature: 42850

Theoretical pI: Translated: 5.33; Mature: 5.33

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLATKFGGAAKAYDQIDAAPEEKARG
CCCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVVK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECHHCCHHH
VGEELEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC
>Mature Secondary Structure 
AKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLATKFGGAAKAYDQIDAAPEEKARG
CCHHHHHCCCCEEECEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCCHHCCC
ITINTAHVEYETANRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSAADGPMPQTREHI
EEEEEEEEEEECCCCEEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLARQVGVPYIVVFLNKCDMVDDAELLELVEMEVRELLSKYDFPGDDTPIIKGSAKLALE
EEEHHCCCCEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEEECCEEEEEE
GDKGELGEVAIMNLADALDTYIPTPERAVDGTFLMPVEDVFSISGRGTVVTGRIERGVVK
CCCCCCCCCHHHHHHHHHHHHCCCCCHHCCCEEEEEHHHHHCCCCCCEEEEECHHCCHHH
VGEELEIVGIKATQKTTCTGVEMFRKLLDQGQAGDNVGILLRGTKREDVERGQVLCKPGS
CCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCEEECCCC
IKPHTHFTGEVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGSIELPADKEMVMPGDNVS
CCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCEEEECCCCCCEEECCCCCE
ITVKLIAPIAMEEGLRFAIREGGRTVGAGVVAKIIE
EEEEEEECHHHHCCCEEEECCCCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GTP; H2O

Specific reaction: GTP + H2O = GDP + phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA