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The map label for this gene is mlaE [H]

Identifier: 187930306

GI number: 187930306

Start: 3383573

End: 3384340

Strand: Reverse

Name: mlaE [H]

Synonym: Rpic_3239

Alternate gene names: 187930306

Gene position: 3384340-3383573 (Counterclockwise)

Preceding gene: 187930307

Following gene: 187930305

Centisome position: 85.84

GC content: 63.02

Gene sequence:

>768_bases
ATGATTTCCACCATCGGCCGCTTTGTTCTGGTGCTGATCAGCCGCTTCGGCTATGCCGCCCGCACGTTGATTTCGCTCAT
TGGCGCTTCGGGCGACGTGCTCCGTCGCCCGCGCCTGGTGATCGAGCAACTGCGCTTCATCGGCAATGATTCGTTCATCA
TCATTGCTGTGTCGGGTCTGTTTGTTGGCTTCGTGCTTGGTTTGCAGGGCTACAACACGCTTAACCGCTACGGCTCCGAG
CAGGCGCTGGGTTTGCTGGTGGCGCTGTCGCTGGTGCGCGAGCTGGGGCCGGTGGTGACGGCCCTGCTGTTTGCCGGTCG
CGCCGGTACGTCGCTCACGGCCGAGATTGGCCTGATGAAAGCCGGCGAGCAGCTGATCGCCATGGAAATGATGGCGGTCG
ATCCGCTGGCCCGCGTGCTGGCGCCGCGTTTCTGGGCGGGCTTCATCGCCATGCCGCTGCTGGCCGCGATTTTCAGTGCG
GTGGGGATTCTGGGCGGATACTTCGTCGGGGTCGGACTGATTGGCGTGGACCCCGGAGCGTTCTGGTCGCAGATGCAGGC
CGGCGTGGATGTCATGGACGACGTGCTCAACGGCGTCATCAAGAGTGTCGTGTTTGGTGTGGCGGTCACCTTCATTGCGC
TGTATCAGGGTTATGAGGCGAAGGCCACGCCGGAAGGCGTCTCGCGCGCGACTACCCGGACGGTGGTGATCGCGTCGCTC
ACCGTGCTGGCGCTGGATTTCGTGCTGACGGCACTGATGTTCAGCTGA

Upstream 100 bases:

>100_bases
GTCAGTTCGTGCACGGAGAGGCGGACGGTCCCGTGCCGTTCCACTATCCCGGGGCACCGCTGGCAGATGACTTCGGTCTG
GGCAAGAGCGGAGGCGCACG

Downstream 100 bases:

>100_bases
GCGGCCGGTTGTCGTTAGCGTTCAACGAGAAAAGATCATGCGTAAAAGCGTCCTCGATTTCTGGGTCGGCCTGTTCGTTC
TGGTGGGCATCATTGCGCTG

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISTIGRFVLVLISRFGYAARTLISLIGASGDVLRRPRLVIEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYNTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLIAMEMMAVDPLARVLAPRFWAGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDPGAFWSQMQAGVDVMDDVLNGVIKSVVFGVAVTFIALYQGYEAKATPEGVSRATTRTVVIASL
TVLALDFVLTALMFS

Sequences:

>Translated_255_residues
MISTIGRFVLVLISRFGYAARTLISLIGASGDVLRRPRLVIEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYNTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLIAMEMMAVDPLARVLAPRFWAGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDPGAFWSQMQAGVDVMDDVLNGVIKSVVFGVAVTFIALYQGYEAKATPEGVSRATTRTVVIASL
TVLALDFVLTALMFS
>Mature_255_residues
MISTIGRFVLVLISRFGYAARTLISLIGASGDVLRRPRLVIEQLRFIGNDSFIIIAVSGLFVGFVLGLQGYNTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLIAMEMMAVDPLARVLAPRFWAGFIAMPLLAAIFSA
VGILGGYFVGVGLIGVDPGAFWSQMQAGVDVMDDVLNGVIKSVVFGVAVTFIALYQGYEAKATPEGVSRATTRTVVIASL
TVLALDFVLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=253, Percent_Identity=57.3122529644269, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 26955; Mature: 26955

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]