Click here to switch to the map view.

The map label for this gene is ubiF [H]

Identifier: 187930202

GI number: 187930202

Start: 3283864

End: 3285159

Strand: Reverse

Name: ubiF [H]

Synonym: Rpic_3135

Alternate gene names: 187930202

Gene position: 3285159-3283864 (Counterclockwise)

Preceding gene: 187930206

Following gene: 187930201

Centisome position: 83.33

GC content: 68.6

Gene sequence:

>1296_bases
ATGAATACACCCGCGTTTTCCTCTTCCAAGGCGGCTCACAAGGAGGCTGCGCCTTACCACGTGGCCGTGGTGGGCGGTGG
CATCGTCGGGCGCGCCTGCGCGCTCCGGCTGGCGCAGATCGGCTTGCGCATCGCGCATATCGCAACGCCCGCCCCAGTGT
CCGCAGCCGCCGGGCCGGACGCCTGGGATGCACGCGTCTACGCGTTCTCGTCCAGCTCGCAGGCGCTGCTGGAGCAGTTG
CGCATCTGGCCGGCCCTCGACATGACGCGCGTGCAGCCTGTGCACGACATGCGCATCTTCGGCGACGCCGCCGCCGCACG
CGGCGAACACGCCCATCCCGACCTGCACTTTTCCGCCTACGCAGCCGGCACGCCGCAACTGGCGTGGATTGCCGAATCCT
CGCTGGTCGAGCGTGCGCTGGAAACGGCGCTGCGCTTCGCCCACACGGTGCAGACCTTCCCGCACGCCGCCACCGGCTTC
GAAATGCAAGCCGATGCCGTGCGCCTGACGCTGGCCGACGGGCAGACGATCCGCGCGGAATGCGTGATCGGCGCCGATGG
CAAGAATTCGTGGGTGCGCCAGCAGGCCGGCATCCAGGCCGAAGCGAAGCCGTACCGGCAGCTCGGCGTGGTTGCCAACT
TCCAGGCCGAGCGCTGGCATCAGGACACCGCGTGGCAATGGTTCCTCGGTGCATCCGCCGAGCAGGCCGCCGAAGCGGAT
GACGCCGCGCCGGTACGCGGCGAGATCCTCGCGATGCTGCCGCTGCCCGATCGACGCGTGTCGATGGTGTGGTCGGCCAG
TGAAGATCACGCCCGCGAACTGCTTGCGCTGTCGCCCGAGGCGCTGTGCCGCGCGGTCGAGGCCGCGGCGGGTGGCGCCA
TCGCCGCCCGCTTCGGGCGCTTGTCGAACATCACGCCTGCGCAGGGCTTCCCGCTCGTGCTGCAACACGCCAGCCGGGCG
GTCGCGCCACGTGTGGCGCTGGTGGGCGATGCAGCGCATGTGATTCATCCGCTGGCCGGGCAGGGCATGAACCTCGGGCT
TAGGGATGTGTCGGAAATTGGCCGTGTGATGGCGGAACGTGAAACCATGCGCGATCATGGCGATCTGAGGTTGCTGCGCC
GCTACGAGCGTGCCCGCCGCGAAGACCTCCTTTCGCTCACGGCCGCCACCGATGGTCTGCACAAACTGTTCGCCCTGCCG
GGCGCGCTGCCTCGCACGGTACGCAACGCGGGGATGCGCATGGTGGGCATGACGCCATTTATCAAACGCTTGTTGATCAG
GCACGCACTCGGCTGA

Upstream 100 bases:

>100_bases
GTAAACACCGAGCCGATCTGCGGCGGTGCCCCATATCCGTCGCGACACATTTGCGCGGTCACAAGGGCGCAGCACCCCCC
GCCGCTATAATCGGCGCACC

Downstream 100 bases:

>100_bases
GGCACTCTCAGCGGATTTGTGGTTTTTCGGGGTTCCAGGAGTCGTTATGTCGTTTCGTATTCGGGTGGCGGCCATCGCCT
CTGCGGTGGCAGTGGCAGCC

Product: ubiquinone biosynthesis hydroxylase family protein

Products: 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNTPAFSSSKAAHKEAAPYHVAVVGGGIVGRACALRLAQIGLRIAHIATPAPVSAAAGPDAWDARVYAFSSSSQALLEQL
RIWPALDMTRVQPVHDMRIFGDAAAARGEHAHPDLHFSAYAAGTPQLAWIAESSLVERALETALRFAHTVQTFPHAATGF
EMQADAVRLTLADGQTIRAECVIGADGKNSWVRQQAGIQAEAKPYRQLGVVANFQAERWHQDTAWQWFLGASAEQAAEAD
DAAPVRGEILAMLPLPDRRVSMVWSASEDHARELLALSPEALCRAVEAAAGGAIAARFGRLSNITPAQGFPLVLQHASRA
VAPRVALVGDAAHVIHPLAGQGMNLGLRDVSEIGRVMAERETMRDHGDLRLLRRYERARREDLLSLTAATDGLHKLFALP
GALPRTVRNAGMRMVGMTPFIKRLLIRHALG

Sequences:

>Translated_431_residues
MNTPAFSSSKAAHKEAAPYHVAVVGGGIVGRACALRLAQIGLRIAHIATPAPVSAAAGPDAWDARVYAFSSSSQALLEQL
RIWPALDMTRVQPVHDMRIFGDAAAARGEHAHPDLHFSAYAAGTPQLAWIAESSLVERALETALRFAHTVQTFPHAATGF
EMQADAVRLTLADGQTIRAECVIGADGKNSWVRQQAGIQAEAKPYRQLGVVANFQAERWHQDTAWQWFLGASAEQAAEAD
DAAPVRGEILAMLPLPDRRVSMVWSASEDHARELLALSPEALCRAVEAAAGGAIAARFGRLSNITPAQGFPLVLQHASRA
VAPRVALVGDAAHVIHPLAGQGMNLGLRDVSEIGRVMAERETMRDHGDLRLLRRYERARREDLLSLTAATDGLHKLFALP
GALPRTVRNAGMRMVGMTPFIKRLLIRHALG
>Mature_431_residues
MNTPAFSSSKAAHKEAAPYHVAVVGGGIVGRACALRLAQIGLRIAHIATPAPVSAAAGPDAWDARVYAFSSSSQALLEQL
RIWPALDMTRVQPVHDMRIFGDAAAARGEHAHPDLHFSAYAAGTPQLAWIAESSLVERALETALRFAHTVQTFPHAATGF
EMQADAVRLTLADGQTIRAECVIGADGKNSWVRQQAGIQAEAKPYRQLGVVANFQAERWHQDTAWQWFLGASAEQAAEAD
DAAPVRGEILAMLPLPDRRVSMVWSASEDHARELLALSPEALCRAVEAAAGGAIAARFGRLSNITPAQGFPLVLQHASRA
VAPRVALVGDAAHVIHPLAGQGMNLGLRDVSEIGRVMAERETMRDHGDLRLLRRYERARREDLLSLTAATDGLHKLFALP
GALPRTVRNAGMRMVGMTPFIKRLLIRHALG

Specific function: Oxygenase that introduces the hydroxyl group at carbon five of 2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol resulting in the formation of 2-octaprenyl-3-methyl-5-hydroxy-6-methoxy-1,4- benzoquinol [H]

COG id: COG0654

COG function: function code HC; 2-polyprenyl-6-methoxyphenol hydroxylase and related FAD-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ubiH/COQ6 family [H]

Homologues:

Organism=Homo sapiens, GI32967305, Length=408, Percent_Identity=28.4313725490196, Blast_Score=132, Evalue=5e-31,
Organism=Homo sapiens, GI32967307, Length=291, Percent_Identity=33.3333333333333, Blast_Score=132, Evalue=7e-31,
Organism=Escherichia coli, GI1786883, Length=427, Percent_Identity=35.1288056206089, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI1789273, Length=419, Percent_Identity=32.4582338902148, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1789274, Length=423, Percent_Identity=30.2600472813239, Blast_Score=152, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI25146631, Length=436, Percent_Identity=27.0642201834862, Blast_Score=122, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19920760, Length=410, Percent_Identity=29.0243902439024, Blast_Score=136, Evalue=4e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002938
- InterPro:   IPR003042
- InterPro:   IPR018168
- InterPro:   IPR010971 [H]

Pfam domain/function: PF01494 FAD_binding_3 [H]

EC number: 1.14.13.- [C]

Molecular weight: Translated: 46407; Mature: 46407

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: PS01304 UBIH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPAFSSSKAAHKEAAPYHVAVVGGGIVGRACALRLAQIGLRIAHIATPAPVSAAAGPD
CCCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
AWDARVYAFSSSSQALLEQLRIWPALDMTRVQPVHDMRIFGDAAAARGEHAHPDLHFSAY
CCCCEEEEECCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHCCHHHCCCCCCCCCCEEEEE
AAGTPQLAWIAESSLVERALETALRFAHTVQTFPHAATGFEMQADAVRLTLADGQTIRAE
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEECCCCEEEEE
CVIGADGKNSWVRQQAGIQAEAKPYRQLGVVANFQAERWHQDTAWQWFLGASAEQAAEAD
EEEECCCCCHHHHHHCCCCCCCCHHHHHCCEECCCHHHCCCCCCEEEEECCCHHHHCCCC
DAAPVRGEILAMLPLPDRRVSMVWSASEDHARELLALSPEALCRAVEAAAGGAIAARFGR
CCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCC
LSNITPAQGFPLVLQHASRAVAPRVALVGDAAHVIHPLAGQGMNLGLRDVSEIGRVMAER
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ETMRDHGDLRLLRRYERARREDLLSLTAATDGLHKLFALPGALPRTVRNAGMRMVGMTPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHH
IKRLLIRHALG
HHHHHHHHHCC
>Mature Secondary Structure
MNTPAFSSSKAAHKEAAPYHVAVVGGGIVGRACALRLAQIGLRIAHIATPAPVSAAAGPD
CCCCCCCCCCHHHHCCCCEEEEEECCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCCC
AWDARVYAFSSSSQALLEQLRIWPALDMTRVQPVHDMRIFGDAAAARGEHAHPDLHFSAY
CCCCEEEEECCCHHHHHHHHHHCCCCCCHHCCCHHHHHHHCCHHHCCCCCCCCCCEEEEE
AAGTPQLAWIAESSLVERALETALRFAHTVQTFPHAATGFEMQADAVRLTLADGQTIRAE
ECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEEECCCCEEEEE
CVIGADGKNSWVRQQAGIQAEAKPYRQLGVVANFQAERWHQDTAWQWFLGASAEQAAEAD
EEEECCCCCHHHHHHCCCCCCCCHHHHHCCEECCCHHHCCCCCCEEEEECCCHHHHCCCC
DAAPVRGEILAMLPLPDRRVSMVWSASEDHARELLALSPEALCRAVEAAAGGAIAARFGR
CCCCCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHCC
LSNITPAQGFPLVLQHASRAVAPRVALVGDAAHVIHPLAGQGMNLGLRDVSEIGRVMAER
CCCCCCCCCCHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHH
ETMRDHGDLRLLRRYERARREDLLSLTAATDGLHKLFALPGALPRTVRNAGMRMVGMTPF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHH
IKRLLIRHALG
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! [C]

Specific reaction: @OCTA02.txt*2-Octaprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol! = 2-Octaprenyl-3-Methyl-5-Hydroxy-6-Methoxy-1,4- Benzoquinol [C]

General reaction: Hydroxylation [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 10802164 [H]