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The map label for this gene is mraW [H]

Identifier: 187930165

GI number: 187930165

Start: 3235863

End: 3236822

Strand: Reverse

Name: mraW [H]

Synonym: Rpic_3097

Alternate gene names: 187930165

Gene position: 3236822-3235863 (Counterclockwise)

Preceding gene: 187930166

Following gene: 187930164

Centisome position: 82.1

GC content: 64.27

Gene sequence:

>960_bases
ATGACAACCCAAACCAGTACGGGACTGCGCCATCAGACGGTGCTCTTAGACGAAGCGGTCGATGCGCTCATCTGGCGCGA
CGACGGTATCTATATCGATGGAACCTTCGGGAGAGGCGGGCACAGCCGGCGCATCCTGGAACGGCTGGGGCCTGGCGGCA
GACTTGTCGCCTTCGACAAGGACCCGGCAGCAATCACCGAAGCGGGCACCGTAGAGGATGCCCGCTTCGCTATTGAGCAT
GACAGCTTCGCGCAGATGGCCCGATGCCTGGACGCGCGTGGCGTGGAGCAGGTGGCCGGCGTGCTGCTTGATCTCGGCAT
CAGTTCGCCGCAGATCGACGAAGGCGCACGTGGCTTCTCGTTCCGGATGGATGGCCCGCTCGACATGCGGATGGACACCA
CGCGCGGCATCACCGCAGCCCAATGGCTGGCCGAAGCTGATGAGCGCGACATTGCGAGGGTGATACGGGACTATGGGGAA
GAACGGTTTGCTGTACAGATTGCAAAGGCGATTGTTGCTCGCCGGGGCCAATCCGGGGATCGAGGTCCTCTCGATCGCAC
ATCCGAGCTTGCCGCGCTCGTGGCGCAAGCCGTCAAAACACGCGAGAAGGGCCAAGACCCTGCGACCCGCACCTTTCAAG
CTTTACGGATTCACGTCAATCAAGAGCTTGCGGACCTCGAAACCGGTCTGAAGGCCGCCTTTGACCGTCTGGAACAGGGG
GGACGTCTCGTCGTGATCAGCTTCCATTCGCTGGAAGACCGCATCGTCAAGCGCTTCATGCAGGCGCTGGCGCGCCCGGA
GCAATCCGCCGCGCCGGAACTGCGTCGGGCGCCGCTGCGTGCGCATGAGCTGCCGGCGCCGCAGTTGCGCCTGCTCGGCC
GCGTGAAGCCGTCGGAGGCGGAAGTGTCGGCCAATCCGCGTGCGCGCTCGGCCATCATGCGCGTGGCCGAACGCTGCTGA

Upstream 100 bases:

>100_bases
CGAAGTCTGGGACGCCGCCACGTACACCGCGAAGGAGCAGGCCGCCATGGCGCAAGGCATGCCCGACGCCCTCAAGAATT
TCTCCTTTTGATGACGCGCG

Downstream 100 bases:

>100_bases
CCGACGAGGCCGCCCCATGAACCGCCTGAACATGTTCCTGCTGACTGCGCTGGTGCTGTGCGCGCTGTCGCTGGTCAACG
CGCAGCATCAGGCGCGGCAA

Product: S-adenosyl-methyltransferase MraW

Products: NA

Alternate protein names: 16S rRNA m(4)C1402 methyltransferase; rRNA (cytosine-N(4)-)-methyltransferase RsmH [H]

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTTQTSTGLRHQTVLLDEAVDALIWRDDGIYIDGTFGRGGHSRRILERLGPGGRLVAFDKDPAAITEAGTVEDARFAIEH
DSFAQMARCLDARGVEQVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGE
ERFAVQIAKAIVARRGQSGDRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKAAFDRLEQG
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPELRRAPLRAHELPAPQLRLLGRVKPSEAEVSANPRARSAIMRVAERC

Sequences:

>Translated_319_residues
MTTQTSTGLRHQTVLLDEAVDALIWRDDGIYIDGTFGRGGHSRRILERLGPGGRLVAFDKDPAAITEAGTVEDARFAIEH
DSFAQMARCLDARGVEQVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGE
ERFAVQIAKAIVARRGQSGDRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKAAFDRLEQG
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPELRRAPLRAHELPAPQLRLLGRVKPSEAEVSANPRARSAIMRVAERC
>Mature_318_residues
TTQTSTGLRHQTVLLDEAVDALIWRDDGIYIDGTFGRGGHSRRILERLGPGGRLVAFDKDPAAITEAGTVEDARFAIEHD
SFAQMARCLDARGVEQVAGVLLDLGISSPQIDEGARGFSFRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEE
RFAVQIAKAIVARRGQSGDRGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKAAFDRLEQGG
RLVVISFHSLEDRIVKRFMQALARPEQSAAPELRRAPLRAHELPAPQLRLLGRVKPSEAEVSANPRARSAIMRVAERC

Specific function: Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA [H]

COG id: COG0275

COG function: function code M; Predicted S-adenosylmethionine-dependent methyltransferase involved in cell envelope biogenesis

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmH family [H]

Homologues:

Organism=Homo sapiens, GI165377209, Length=344, Percent_Identity=39.5348837209302, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI165377202, Length=198, Percent_Identity=39.3939393939394, Blast_Score=113, Evalue=3e-25,
Organism=Escherichia coli, GI1786270, Length=314, Percent_Identity=54.4585987261147, Blast_Score=320, Evalue=6e-89,
Organism=Drosophila melanogaster, GI62472493, Length=330, Percent_Identity=36.969696969697, Blast_Score=170, Evalue=9e-43,
Organism=Drosophila melanogaster, GI28571637, Length=330, Percent_Identity=36.969696969697, Blast_Score=170, Evalue=9e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002903 [H]

Pfam domain/function: PF01795 Methyltransf_5 [H]

EC number: NA

Molecular weight: Translated: 35123; Mature: 34992

Theoretical pI: Translated: 7.15; Mature: 7.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTQTSTGLRHQTVLLDEAVDALIWRDDGIYIDGTFGRGGHSRRILERLGPGGRLVAFDK
CCCCCCCCCCHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECC
DPAAITEAGTVEDARFAIEHDSFAQMARCLDARGVEQVAGVLLDLGISSPQIDEGARGFS
CCCHHCCCCCCCHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCE
FRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEERFAVQIAKAIVARRGQSGD
EEECCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
RGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKAAFDRLEQG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPELRRAPLRAHELPAPQLRLLGRVKPSEA
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC
EVSANPRARSAIMRVAERC
CCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TTQTSTGLRHQTVLLDEAVDALIWRDDGIYIDGTFGRGGHSRRILERLGPGGRLVAFDK
CCCCCCCCCHHHHHHHHHHHHHEECCCCEEEECCCCCCCHHHHHHHHHCCCCEEEEECC
DPAAITEAGTVEDARFAIEHDSFAQMARCLDARGVEQVAGVLLDLGISSPQIDEGARGFS
CCCHHCCCCCCCHHHHEECCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCCCCCCCCCE
FRMDGPLDMRMDTTRGITAAQWLAEADERDIARVIRDYGEERFAVQIAKAIVARRGQSGD
EEECCCCCCEECCCCCHHHHHHHHHCCHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCC
RGPLDRTSELAALVAQAVKTREKGQDPATRTFQALRIHVNQELADLETGLKAAFDRLEQG
CCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GRLVVISFHSLEDRIVKRFMQALARPEQSAAPELRRAPLRAHELPAPQLRLLGRVKPSEA
CCEEEEECCCHHHHHHHHHHHHHCCCCCCCCCHHHHCCCHHHCCCCHHHHHHHCCCCCCC
EVSANPRARSAIMRVAERC
CCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA