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The map label for this gene is ampD [H]

Identifier: 187930119

GI number: 187930119

Start: 3184964

End: 3185560

Strand: Reverse

Name: ampD [H]

Synonym: Rpic_3051

Alternate gene names: 187930119

Gene position: 3185560-3184964 (Counterclockwise)

Preceding gene: 187930120

Following gene: 187930115

Centisome position: 80.8

GC content: 64.99

Gene sequence:

>597_bases
ATGCCTGAACGGTTGCACGTCGGCGCTGACGGATGGGTGGATGGCGCGCGCCGCCATCCCTCGCCGAATATCGACGCACG
GCCCGAAGGCATGCCCATCGACCTGATCGTGCTGCACAACATCAGCCTGCCGCCTGCGCAATCCGCGGCCGACTTCGGCA
CCTGCCACGTGCTCGACTTCTTCACCAACACGCTTGACTGCGACGCGCATCCGTACTTCGATCAGTTGCGCGGCGTGCGC
GTGTCGGCGCATTTCTTCGTCCGCCGCACGGGCGAATGCGTGCAATTTGCTTCGTGCGACGCGCGTGCCTGGCATGCCGG
TGCGTCGGACTTCTTCGGCCGCACGCGCTGCAACGATTTCTCGATCGGCATCGAAATCGAGGGCACCGATGATCTGCCCT
TCACGCCTGAGCAGTACGTCGCTACGGCGTCGCTTGTGCGGGCGATCTGTGCGGCGTATCCGATCGCAGCAATCGCCGGT
CACTCGGACATTGCGCCGGGCCGCAAGACGGACCCGGGACCGCATTTCGACTGGGTGCATCTGCGCGCGCTCGGCGGGTT
CAACGCGGCGTTGTTTCCGTATCAGCACGCGCTCTGA

Upstream 100 bases:

>100_bases
TTGCGCTATCGCATGCAGCAGCTTGGCATTCGTGATCCGCGGGACGTTGAAGCGTCGCCGGGCAGCGTCGGTGAACCGGC
CGGAAATGGGCTGAACGGCG

Downstream 100 bases:

>100_bases
CGCGACAACCCCCGTTCGCTCGCCAAGGTAGAAAGGCGGCGCACCTCGGCGCGGCGCCGGCACACGGCGGTAAGGCGCCG
ACGCCTCACGGTCTGCGGCT

Product: N-acetyl-anhydromuranmyl-L-alanine amidase

Products: NA

Alternate protein names: N-acetylmuramoyl-L-alanine amidase [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MPERLHVGADGWVDGARRHPSPNIDARPEGMPIDLIVLHNISLPPAQSAADFGTCHVLDFFTNTLDCDAHPYFDQLRGVR
VSAHFFVRRTGECVQFASCDARAWHAGASDFFGRTRCNDFSIGIEIEGTDDLPFTPEQYVATASLVRAICAAYPIAAIAG
HSDIAPGRKTDPGPHFDWVHLRALGGFNAALFPYQHAL

Sequences:

>Translated_198_residues
MPERLHVGADGWVDGARRHPSPNIDARPEGMPIDLIVLHNISLPPAQSAADFGTCHVLDFFTNTLDCDAHPYFDQLRGVR
VSAHFFVRRTGECVQFASCDARAWHAGASDFFGRTRCNDFSIGIEIEGTDDLPFTPEQYVATASLVRAICAAYPIAAIAG
HSDIAPGRKTDPGPHFDWVHLRALGGFNAALFPYQHAL
>Mature_197_residues
PERLHVGADGWVDGARRHPSPNIDARPEGMPIDLIVLHNISLPPAQSAADFGTCHVLDFFTNTLDCDAHPYFDQLRGVRV
SAHFFVRRTGECVQFASCDARAWHAGASDFFGRTRCNDFSIGIEIEGTDDLPFTPEQYVATASLVRAICAAYPIAAIAGH
SDIAPGRKTDPGPHFDWVHLRALGGFNAALFPYQHAL

Specific function: Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha- amino group of the L-alanine in degradation products containing an anhydr

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1786300, Length=176, Percent_Identity=53.4090909090909, Blast_Score=182, Evalue=1e-47,
Organism=Escherichia coli, GI1787092, Length=157, Percent_Identity=36.3057324840764, Blast_Score=72, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 21654; Mature: 21523

Theoretical pI: Translated: 6.10; Mature: 6.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPERLHVGADGWVDGARRHPSPNIDARPEGMPIDLIVLHNISLPPAQSAADFGTCHVLDF
CCCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEHHHH
FTNTLDCDAHPYFDQLRGVRVSAHFFVRRTGECVQFASCDARAWHAGASDFFGRTRCNDF
HHCCCCCCCCCCHHHHCCEEEEEEEEEECCCCEEEECCCCCCHHCCCCHHHCCCCCCCCE
SIGIEIEGTDDLPFTPEQYVATASLVRAICAAYPIAAIAGHSDIAPGRKTDPGPHFDWVH
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LRALGGFNAALFPYQHAL
EEEECCCCEEECCHHHCC
>Mature Secondary Structure 
PERLHVGADGWVDGARRHPSPNIDARPEGMPIDLIVLHNISLPPAQSAADFGTCHVLDF
CCEEECCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEHHHH
FTNTLDCDAHPYFDQLRGVRVSAHFFVRRTGECVQFASCDARAWHAGASDFFGRTRCNDF
HHCCCCCCCCCCHHHHCCEEEEEEEEEECCCCEEEECCCCCCHHCCCCHHHCCCCCCCCE
SIGIEIEGTDDLPFTPEQYVATASLVRAICAAYPIAAIAGHSDIAPGRKTDPGPHFDWVH
EEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEE
LRALGGFNAALFPYQHAL
EEEECCCCEEECCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677609; 8419294 [H]