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The map label for this gene is grpE

Identifier: 187929942

GI number: 187929942

Start: 2979550

End: 2980197

Strand: Reverse

Name: grpE

Synonym: Rpic_2874

Alternate gene names: 187929942

Gene position: 2980197-2979550 (Counterclockwise)

Preceding gene: 187929944

Following gene: 187929941

Centisome position: 75.59

GC content: 65.28

Gene sequence:

>648_bases
ATGAAACACACTTCGGACACCCCCTCGAACTCGGACATGCCATCGGACAGCCAAGCCACCCAGCCGAACGCATCGGCGAC
GGGCCAAGCGGCCCATGCCTATTCGAGTCAGGCGCAGCGCGCAAGCGCCGACGCCCAGGCTGTCGCCGGTGACGAAGCCG
CCGTGGCAGAAGCTGTGGCCGACGTGGACGTCGCTGAACTGCGTCGTCAACTGGAGGCCGCAGAAGAAAAGGCTCGCCAG
AATTACGAAAACTGGGCGCGCGCGACGGCCGAAGGCGAGAACATCCGCCGCCGTGGTCAGGACGATGTGGCCAAGGCCCA
CAAGTTCGCCATCGAAGGCTTTGCCGAATACTTGCTGCCGGTGATGGACAGCCTGCAAGCCGCCTTGGCGGACACGTCGG
GCGACGCCACCAAGCTGCGTGAGGGCGTTGAACTCACGCTCAAGCAGCTCTACGCCGCGTTCGAGAAGGGCCGTGTGACC
GAGCTGAACCCGGTGGGTGAAAAGTTTGATCCGCACCGCCATCAAGCTATCTCGATGGTGCCGGCTGACCAGGAAGCCAA
CACCGTGGTGGCGGTGCTCCAGCGCGGGTACACGCTGGCCGACCGCGTGCTGCGCCCGGCCCTCGTGACGGTGGCGGCTC
CCAAGTGA

Upstream 100 bases:

>100_bases
AAGCGCGCCAAATGCCCCATAACGGGGACAAATCGTTTGTAAAAATCCAGCGGATCGATTCCGGGCACATGCCGTGCGTC
GATCCCCTTTATCCAGCGCT

Downstream 100 bases:

>100_bases
GCCGACGCGCCCCATGATCCAGAACATAGCCACTTCGCACGACGCCCACGCCGCGATCCCGGTCGACAAGACCGCGCTCG
CGGCGTTCGGCATGCGCGAG

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MKHTSDTPSNSDMPSDSQATQPNASATGQAAHAYSSQAQRASADAQAVAGDEAAVAEAVADVDVAELRRQLEAAEEKARQ
NYENWARATAEGENIRRRGQDDVAKAHKFAIEGFAEYLLPVMDSLQAALADTSGDATKLREGVELTLKQLYAAFEKGRVT
ELNPVGEKFDPHRHQAISMVPADQEANTVVAVLQRGYTLADRVLRPALVTVAAPK

Sequences:

>Translated_215_residues
MKHTSDTPSNSDMPSDSQATQPNASATGQAAHAYSSQAQRASADAQAVAGDEAAVAEAVADVDVAELRRQLEAAEEKARQ
NYENWARATAEGENIRRRGQDDVAKAHKFAIEGFAEYLLPVMDSLQAALADTSGDATKLREGVELTLKQLYAAFEKGRVT
ELNPVGEKFDPHRHQAISMVPADQEANTVVAVLQRGYTLADRVLRPALVTVAAPK
>Mature_215_residues
MKHTSDTPSNSDMPSDSQATQPNASATGQAAHAYSSQAQRASADAQAVAGDEAAVAEAVADVDVAELRRQLEAAEEKARQ
NYENWARATAEGENIRRRGQDDVAKAHKFAIEGFAEYLLPVMDSLQAALADTSGDATKLREGVELTLKQLYAAFEKGRVT
ELNPVGEKFDPHRHQAISMVPADQEANTVVAVLQRGYTLADRVLRPALVTVAAPK

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family

Homologues:

Organism=Homo sapiens, GI24308295, Length=147, Percent_Identity=38.0952380952381, Blast_Score=96, Evalue=3e-20,
Organism=Homo sapiens, GI40255109, Length=138, Percent_Identity=36.9565217391304, Blast_Score=77, Evalue=1e-14,
Organism=Escherichia coli, GI1788967, Length=159, Percent_Identity=44.0251572327044, Blast_Score=111, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17552458, Length=142, Percent_Identity=35.2112676056338, Blast_Score=80, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6324806, Length=158, Percent_Identity=32.9113924050633, Blast_Score=86, Evalue=4e-18,
Organism=Drosophila melanogaster, GI24653432, Length=159, Percent_Identity=35.2201257861635, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): GRPE_RALPJ (B2UBP7)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001900429.1
- ProteinModelPortal:   B2UBP7
- SMR:   B2UBP7
- GeneID:   6287079
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2874
- HOGENOM:   HBG732630
- OMA:   DHANEES
- ProtClustDB:   PRK14139
- GO:   GO:0005739
- HAMAP:   MF_01151
- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012
- Gene3D:   G3DSA:3.90.20.20
- Gene3D:   G3DSA:2.30.22.10
- PANTHER:   PTHR21237
- PRINTS:   PR00773

Pfam domain/function: PF01025 GrpE; SSF51064 GrpE_head

EC number: NA

Molecular weight: Translated: 23084; Mature: 23084

Theoretical pI: Translated: 4.85; Mature: 4.85

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKHTSDTPSNSDMPSDSQATQPNASATGQAAHAYSSQAQRASADAQAVAGDEAAVAEAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHH
DVDVAELRRQLEAAEEKARQNYENWARATAEGENIRRRGQDDVAKAHKFAIEGFAEYLLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VMDSLQAALADTSGDATKLREGVELTLKQLYAAFEKGRVTELNPVGEKFDPHRHQAISMV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCC
PADQEANTVVAVLQRGYTLADRVLRPALVTVAAPK
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKHTSDTPSNSDMPSDSQATQPNASATGQAAHAYSSQAQRASADAQAVAGDEAAVAEAVA
CCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCHHHHHHHHH
DVDVAELRRQLEAAEEKARQNYENWARATAEGENIRRRGQDDVAKAHKFAIEGFAEYLLP
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHH
VMDSLQAALADTSGDATKLREGVELTLKQLYAAFEKGRVTELNPVGEKFDPHRHQAISMV
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHCC
PADQEANTVVAVLQRGYTLADRVLRPALVTVAAPK
CCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA