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The map label for this gene is kefA [H]

Identifier: 187929789

GI number: 187929789

Start: 2832847

End: 2834220

Strand: Reverse

Name: kefA [H]

Synonym: Rpic_2719

Alternate gene names: 187929789

Gene position: 2834220-2832847 (Counterclockwise)

Preceding gene: 187929790

Following gene: 187929788

Centisome position: 71.89

GC content: 64.19

Gene sequence:

>1374_bases
ATGAACGGATTGCCGCTGAACCTGAACCACATCGCGCTGGGCCACATGATCGACGACATTGTCTTCGATCTGGGTGGCCC
TCGCTTTATCTGGCAGCTGGGTGTGCTGCTGCTGGTGCTCGCGGCCGCCTGGCTGATAGCGCGGCCGATTTCGCGGCGCT
TGCACACGGCCCATGTGAACGACACGTTCGCCCTGCGTTTTGCCTGGTCGAGCCTGGAGCGGGCGATGTTCCCGCTGATC
GGCTGGCTGCTGGTGGTGGGCGCGCGCTACGCCCTGATCGGCACCATGCCGATCAGCGTGTTCCGGCTGGCTGCGGTGCC
GCTGTTTGGCCTGGCGATCCTCTATCTCGGCTTCTACGTGCTGCGTCGCGTGCTGTCGGCCAACGGCGAACTGCACGGCA
TGCTGGTCGTCGCCGAGCGCGTGCTGACCACGCTCATGTGGATGGGCATGGTGCTCTACGTGCTGGGTGTGCTGGGCGAT
GTGGTGGACTATCTTGACGGCATTCGCTTTGCGCTGGGCGGCAAGCAGAAGGTGAGCCTTGCCGCCATGCTGATGGGCGT
GGTCTGGATTCTCGTCACCGTGCTCGTGGCGATGTGGTTCGGCTCGTGGCTGGATAGCCGCATCACGCGTGCTTCCGCGA
TTGATGCCAACCTGAAGGTGGTGCTATCGCGCGTGACCAAGGCGGTGCTGCTGCTGGTGTCGTTGCTGCTGAGCCTGTCG
CTGGTTGGCATCGACCTGACGGTGCTGTCGGTGTTTGGCGGCGCGCTGGGCGTTGGCCTGGGCTTCGGCTTGCAGAAGAT
CGCCAGCAATTACGTCTCGGGCTTCATCATCCTGCTCGAGCGCTCACTCAAGCTGGGCGATCAGATTACCGTCAGCACGT
ACACCGGCATCGTCACGCAGATCCGCACCCGCTATACCGTGGTGCGCAATGGCGACGGCGATACGCTGGTTCCGAACGAA
CTGATGGTCGCGCAGGCCGTGCAAAACCATAACGAGCGCGGCACCGTGCGCGTGGCGGTGCGCGTGCAGGCATCGTATGC
CGCCGACCCCGAAGCCGTGCTGGCGCTACTGGTGCAATGCGCCGAGGGCATCCCGCGTGTTCTGCCCGAGCCGGCGCCCG
CCGCTTTCATGGCTCTGTTCGCCGACAGCGGTATCGAGTACGAACTTGGCGTGTGGATCGCCGATCCGCACAATGGCAAG
CTGGGCGTGCAGTCCGACCTGAACCGCGCCATCTATCGTCGCTTCAAGGAGGCTGGCATCGAGATTCCGTACCCACAACG
CGAAGTGCGTGTCCTGGGCTCGCTGGCCTTGCAGGGCGCTTCAGGCACTTCGGGTGATGTGGCGGCCTCTTCCAGCGCAC
CCCTGCCGACTTAG

Upstream 100 bases:

>100_bases
TCGTGCGCGGCGACGCCGATCTCGCCCAGCGCTATGCCGCCAACTGGCAGAAGCATGCGGGTGACGCGTTGCCCTATCGA
GCCCAACAACCGGCCGCCCA

Downstream 100 bases:

>100_bases
CACACCTGCGGGCGGAACGGTGCCCACTTGCCTGAGGTCAAACCAGCGGTTTTCCCCCATGGAATCGAGGGGGTATTCGG
TTAAAATGTTGGATGATCCC

Product: mechanosensitive ion channel MscS

Products: NA

Alternate protein names: Protein AefA [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNGLPLNLNHIALGHMIDDIVFDLGGPRFIWQLGVLLLVLAAAWLIARPISRRLHTAHVNDTFALRFAWSSLERAMFPLI
GWLLVVGARYALIGTMPISVFRLAAVPLFGLAILYLGFYVLRRVLSANGELHGMLVVAERVLTTLMWMGMVLYVLGVLGD
VVDYLDGIRFALGGKQKVSLAAMLMGVVWILVTVLVAMWFGSWLDSRITRASAIDANLKVVLSRVTKAVLLLVSLLLSLS
LVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERSLKLGDQITVSTYTGIVTQIRTRYTVVRNGDGDTLVPNE
LMVAQAVQNHNERGTVRVAVRVQASYAADPEAVLALLVQCAEGIPRVLPEPAPAAFMALFADSGIEYELGVWIADPHNGK
LGVQSDLNRAIYRRFKEAGIEIPYPQREVRVLGSLALQGASGTSGDVAASSSAPLPT

Sequences:

>Translated_457_residues
MNGLPLNLNHIALGHMIDDIVFDLGGPRFIWQLGVLLLVLAAAWLIARPISRRLHTAHVNDTFALRFAWSSLERAMFPLI
GWLLVVGARYALIGTMPISVFRLAAVPLFGLAILYLGFYVLRRVLSANGELHGMLVVAERVLTTLMWMGMVLYVLGVLGD
VVDYLDGIRFALGGKQKVSLAAMLMGVVWILVTVLVAMWFGSWLDSRITRASAIDANLKVVLSRVTKAVLLLVSLLLSLS
LVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERSLKLGDQITVSTYTGIVTQIRTRYTVVRNGDGDTLVPNE
LMVAQAVQNHNERGTVRVAVRVQASYAADPEAVLALLVQCAEGIPRVLPEPAPAAFMALFADSGIEYELGVWIADPHNGK
LGVQSDLNRAIYRRFKEAGIEIPYPQREVRVLGSLALQGASGTSGDVAASSSAPLPT
>Mature_457_residues
MNGLPLNLNHIALGHMIDDIVFDLGGPRFIWQLGVLLLVLAAAWLIARPISRRLHTAHVNDTFALRFAWSSLERAMFPLI
GWLLVVGARYALIGTMPISVFRLAAVPLFGLAILYLGFYVLRRVLSANGELHGMLVVAERVLTTLMWMGMVLYVLGVLGD
VVDYLDGIRFALGGKQKVSLAAMLMGVVWILVTVLVAMWFGSWLDSRITRASAIDANLKVVLSRVTKAVLLLVSLLLSLS
LVGIDLTVLSVFGGALGVGLGFGLQKIASNYVSGFIILLERSLKLGDQITVSTYTGIVTQIRTRYTVVRNGDGDTLVPNE
LMVAQAVQNHNERGTVRVAVRVQASYAADPEAVLALLVQCAEGIPRVLPEPAPAAFMALFADSGIEYELGVWIADPHNGK
LGVQSDLNRAIYRRFKEAGIEIPYPQREVRVLGSLALQGASGTSGDVAASSSAPLPT

Specific function: Mechanosensitive channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell, although it does not appear to have a major role in osmolarity regulation. Form

COG id: COG3264

COG function: function code M; Small-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscS (TC 1.A.23) family [H]

Homologues:

Organism=Escherichia coli, GI1786670, Length=192, Percent_Identity=34.8958333333333, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI2367355, Length=277, Percent_Identity=28.8808664259928, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1789291, Length=275, Percent_Identity=24.7272727272727, Blast_Score=77, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010920
- InterPro:   IPR011066
- InterPro:   IPR006685
- InterPro:   IPR006686
- InterPro:   IPR011014 [H]

Pfam domain/function: PF00924 MS_channel [H]

EC number: NA

Molecular weight: Translated: 49320; Mature: 49320

Theoretical pI: Translated: 9.39; Mature: 9.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure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CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9278503; 10202137 [H]