BacMap

Definition	Ralstonia pickettii 12J chromosome chromosome 1, complete sequence.
Accession	NC_010682
Length	3,942,557

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The map label for this gene is dkgB [H]

Identifier: 187929766

GI number: 187929766

Start: 2808396

End: 2809202

Strand: Reverse

Name: dkgB [H]

Synonym: Rpic_2695

Alternate gene names: 187929766

Gene position: 2809202-2808396 (Counterclockwise)

Preceding gene: 187929767

Following gene: 187929761

Centisome position: 71.25

GC content: 63.32

Gene sequence:

>807_bases
ATGAACAAGATTCCCGCTTTCGGCCTCGGCACGTTCCGCCTGAAGGGCCAGGTCGTCATCGACTCGGTTCGCAATGGCCT
CGAACTTGGCTACCGCGTCATCGACACCGCGCAAATCTACGGCAATGAAGCGGAGGTAGGCGAGGCCATTGCCGGCTCCG
GCGTGCGCCGCGAAGACCTGTTCCTCACGACCAAGATCTGGGTCGACAACTACGCCAGGGACAAGCTCGCGGCGAGTCTC
GAAGACAGCCTCGCCAAGCTGCGCACCGATTACGTCGACCTCACGCTGATCCACTGGCCTGCGCCCGACAACGGCGTGTC
GATCGAAGAATTCATGACCGCGTTGGCCGAAGCCAAGGCCCGGGGTTTGACGCGCGAGATCGGCGTCTCGAACTTCAATA
TCGCCCTGACGCAGCAGGCGATTGCGGTGGCTGGCAAAGACGCCATCGCCACCAACCAGATCGAGCTGAGCCCGTATCTG
CAGAACCGCAAGCTCGTCGAATTTCTGCAGCGCGAGGGCATTCACGTCACGTCGTACATGACGCTCGCCTACGGCAAGGT
GCTGGGCGATCCGGTGATCGGCGCGATCGCACAGCGGCACGAAGCGACGCCGGCGCAGGTCGTGCTGGCGTGGGCGATGC
AGCTCGGCTACTCGGTCATCCCGTCGTCAACCAAGCGAGAGCATCTTGCGAGCAACCTGCGCGCGCAAACGCTGCACCTG
ACGGACGAAGACATGGCTCAGATCGCCGCGCTTGAGCGCAACGGTCGCGAGGTTTCACCGGACGGGCTGGCGCCGCAGTG
GGATTGA

Upstream 100 bases:

>100_bases
TGATTGCTGTGGCAGGCCTTGCGCTGGTGGGGCTGCACGTCGTGCAGCGTCGGGCGCGCTTCGCCATGAACCCCTGATTC
GATTTCCCCTGGAGCACAAG

Downstream 100 bases:

>100_bases
ACATCGCAGCACCGACGCAATAGAAAAAGCCGCTCCGAAGAGCGGCTTTTTTACGTCGATCGAGCGCTTGGCCCGTGTTG
CATCAGTCGTCCAGTTGCGA

Product: 2,5-diketo-D-gluconate reductase B

Products: NA

Alternate protein names: 2,5-DKG reductase B; 2,5-DKGR B; 25DKGR-B; AKR5D [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNKIPAFGLGTFRLKGQVVIDSVRNGLELGYRVIDTAQIYGNEAEVGEAIAGSGVRREDLFLTTKIWVDNYARDKLAASL
EDSLAKLRTDYVDLTLIHWPAPDNGVSIEEFMTALAEAKARGLTREIGVSNFNIALTQQAIAVAGKDAIATNQIELSPYL
QNRKLVEFLQREGIHVTSYMTLAYGKVLGDPVIGAIAQRHEATPAQVVLAWAMQLGYSVIPSSTKREHLASNLRAQTLHL
TDEDMAQIAALERNGREVSPDGLAPQWD

Sequences:

>Translated_268_residues
MNKIPAFGLGTFRLKGQVVIDSVRNGLELGYRVIDTAQIYGNEAEVGEAIAGSGVRREDLFLTTKIWVDNYARDKLAASL
EDSLAKLRTDYVDLTLIHWPAPDNGVSIEEFMTALAEAKARGLTREIGVSNFNIALTQQAIAVAGKDAIATNQIELSPYL
QNRKLVEFLQREGIHVTSYMTLAYGKVLGDPVIGAIAQRHEATPAQVVLAWAMQLGYSVIPSSTKREHLASNLRAQTLHL
TDEDMAQIAALERNGREVSPDGLAPQWD
>Mature_268_residues
MNKIPAFGLGTFRLKGQVVIDSVRNGLELGYRVIDTAQIYGNEAEVGEAIAGSGVRREDLFLTTKIWVDNYARDKLAASL
EDSLAKLRTDYVDLTLIHWPAPDNGVSIEEFMTALAEAKARGLTREIGVSNFNIALTQQAIAVAGKDAIATNQIELSPYL
QNRKLVEFLQREGIHVTSYMTLAYGKVLGDPVIGAIAQRHEATPAQVVLAWAMQLGYSVIPSSTKREHLASNLRAQTLHL
TDEDMAQIAALERNGREVSPDGLAPQWD

Specific function: Catalyzes the reduction of 2,5-diketo-D-gluconic acid (25DKG) to 2-keto-L-gulonic acid (2KLG) [H]

COG id: COG0656

COG function: function code R; Aldo/keto reductases, related to diketogulonate reductase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aldo/keto reductase family [H]

Homologues:

Organism=Homo sapiens, GI310109922, Length=280, Percent_Identity=33.9285714285714, Blast_Score=160, Evalue=1e-39,
Organism=Homo sapiens, GI24497577, Length=290, Percent_Identity=32.7586206896552, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI5174391, Length=290, Percent_Identity=32.7586206896552, Blast_Score=157, Evalue=9e-39,
Organism=Homo sapiens, GI223468663, Length=286, Percent_Identity=34.6153846153846, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI310109920, Length=297, Percent_Identity=32.3232323232323, Blast_Score=155, Evalue=3e-38,
Organism=Homo sapiens, GI45446745, Length=297, Percent_Identity=32.3232323232323, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI4503285, Length=297, Percent_Identity=32.3232323232323, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI93277124, Length=301, Percent_Identity=31.5614617940199, Blast_Score=154, Evalue=7e-38,
Organism=Homo sapiens, GI5453543, Length=297, Percent_Identity=31.986531986532, Blast_Score=153, Evalue=1e-37,
Organism=Homo sapiens, GI24497585, Length=301, Percent_Identity=31.5614617940199, Blast_Score=150, Evalue=1e-36,
Organism=Homo sapiens, GI5174695, Length=295, Percent_Identity=34.2372881355932, Blast_Score=147, Evalue=7e-36,
Organism=Homo sapiens, GI24497583, Length=297, Percent_Identity=31.986531986532, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI291291012, Length=270, Percent_Identity=34.4444444444444, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI4502049, Length=287, Percent_Identity=34.4947735191638, Blast_Score=143, Evalue=2e-34,
Organism=Homo sapiens, GI300116273, Length=271, Percent_Identity=33.5793357933579, Blast_Score=132, Evalue=3e-31,
Organism=Homo sapiens, GI300116271, Length=277, Percent_Identity=32.8519855595668, Blast_Score=123, Evalue=2e-28,
Organism=Homo sapiens, GI310109924, Length=162, Percent_Identity=32.0987654320988, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI207028673, Length=124, Percent_Identity=36.2903225806452, Blast_Score=81, Evalue=1e-15,
Organism=Homo sapiens, GI310109926, Length=124, Percent_Identity=36.2903225806452, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1786400, Length=265, Percent_Identity=69.0566037735849, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI87082198, Length=265, Percent_Identity=38.1132075471698, Blast_Score=174, Evalue=8e-45,
Organism=Escherichia coli, GI1788081, Length=261, Percent_Identity=31.8007662835249, Blast_Score=114, Evalue=8e-27,
Organism=Escherichia coli, GI48994888, Length=278, Percent_Identity=29.4964028776978, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1787674, Length=278, Percent_Identity=26.6187050359712, Blast_Score=82, Evalue=4e-17,
Organism=Escherichia coli, GI1789199, Length=329, Percent_Identity=26.1398176291793, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87081735, Length=272, Percent_Identity=25.7352941176471, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17552492, Length=266, Percent_Identity=33.4586466165414, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17537077, Length=287, Percent_Identity=35.191637630662, Blast_Score=151, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI17561298, Length=274, Percent_Identity=31.021897810219, Blast_Score=148, Evalue=3e-36,
Organism=Caenorhabditis elegans, GI17537075, Length=289, Percent_Identity=34.6020761245675, Blast_Score=147, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17550248, Length=284, Percent_Identity=33.0985915492958, Blast_Score=145, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17566692, Length=286, Percent_Identity=32.5174825174825, Blast_Score=144, Evalue=4e-35,
Organism=Caenorhabditis elegans, GI71998625, Length=268, Percent_Identity=33.5820895522388, Blast_Score=139, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17561300, Length=273, Percent_Identity=30.03663003663, Blast_Score=133, Evalue=8e-32,
Organism=Caenorhabditis elegans, GI17537079, Length=295, Percent_Identity=30.1694915254237, Blast_Score=132, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17564128, Length=291, Percent_Identity=29.2096219931272, Blast_Score=125, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17538386, Length=268, Percent_Identity=30.5970149253731, Blast_Score=124, Evalue=7e-29,
Organism=Caenorhabditis elegans, GI17562292, Length=270, Percent_Identity=31.8518518518519, Blast_Score=120, Evalue=6e-28,
Organism=Saccharomyces cerevisiae, GI6324694, Length=288, Percent_Identity=35.0694444444444, Blast_Score=155, Evalue=5e-39,
Organism=Saccharomyces cerevisiae, GI6320576, Length=278, Percent_Identity=36.3309352517986, Blast_Score=152, Evalue=8e-38,
Organism=Saccharomyces cerevisiae, GI6321896, Length=316, Percent_Identity=33.2278481012658, Blast_Score=147, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322556, Length=251, Percent_Identity=31.0756972111554, Blast_Score=137, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6320079, Length=275, Percent_Identity=30.5454545454545, Blast_Score=99, Evalue=7e-22,
Organism=Saccharomyces cerevisiae, GI6319625, Length=273, Percent_Identity=29.3040293040293, Blast_Score=97, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24662789, Length=287, Percent_Identity=33.7979094076655, Blast_Score=150, Evalue=7e-37,
Organism=Drosophila melanogaster, GI24644950, Length=299, Percent_Identity=33.7792642140468, Blast_Score=140, Evalue=1e-33,
Organism=Drosophila melanogaster, GI24662785, Length=293, Percent_Identity=35.8361774744027, Blast_Score=139, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24662781, Length=292, Percent_Identity=34.9315068493151, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24657054, Length=286, Percent_Identity=33.2167832167832, Blast_Score=133, Evalue=1e-31,
Organism=Drosophila melanogaster, GI221378297, Length=319, Percent_Identity=31.0344827586207, Blast_Score=132, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24663317, Length=290, Percent_Identity=31.7241379310345, Blast_Score=130, Evalue=1e-30,
Organism=Drosophila melanogaster, GI45553081, Length=286, Percent_Identity=31.1188811188811, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI20129731, Length=287, Percent_Identity=31.7073170731707, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI21356425, Length=274, Percent_Identity=29.9270072992701, Blast_Score=117, Evalue=6e-27,
Organism=Drosophila melanogaster, GI281366140, Length=260, Percent_Identity=30.3846153846154, Blast_Score=109, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001395
- InterPro:   IPR018170
- InterPro:   IPR020471
- InterPro:   IPR023210 [H]

Pfam domain/function: PF00248 Aldo_ket_red [H]

EC number: =1.1.1.274 [H]

Molecular weight: Translated: 29358; Mature: 29358

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00062 ALDOKETO_REDUCTASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKIPAFGLGTFRLKGQVVIDSVRNGLELGYRVIDTAQIYGNEAEVGEAIAGSGVRREDL
CCCCCCCCCEEEEEECCEEEHHHHHHHHHCHHHEEHHHHCCCHHHHHHHHHCCCCCCHHE
FLTTKIWVDNYARDKLAASLEDSLAKLRTDYVDLTLIHWPAPDNGVSIEEFMTALAEAKA
EEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
RGLTREIGVSNFNIALTQQAIAVAGKDAIATNQIELSPYLQNRKLVEFLQREGIHVTSYM
HCCHHHHCCCCCEEEEHHHHHHHCCCCCEECCEEEECHHHCCCHHHHHHHHCCCEEEHHH
TLAYGKVLGDPVIGAIAQRHEATPAQVVLAWAMQLGYSVIPSSTKREHLASNLRAQTLHL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCHHHHHHHHCCHHEEEEC
TDEDMAQIAALERNGREVSPDGLAPQWD
CHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKIPAFGLGTFRLKGQVVIDSVRNGLELGYRVIDTAQIYGNEAEVGEAIAGSGVRREDL
CCCCCCCCCEEEEEECCEEEHHHHHHHHHCHHHEEHHHHCCCHHHHHHHHHCCCCCCHHE
FLTTKIWVDNYARDKLAASLEDSLAKLRTDYVDLTLIHWPAPDNGVSIEEFMTALAEAKA
EEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCHHHHHHHHHHHHH
RGLTREIGVSNFNIALTQQAIAVAGKDAIATNQIELSPYLQNRKLVEFLQREGIHVTSYM
HCCHHHHCCCCCEEEEHHHHHHHCCCCCEECCEEEECHHHCCCHHHHHHHHCCCEEEHHH
TLAYGKVLGDPVIGAIAQRHEATPAQVVLAWAMQLGYSVIPSSTKREHLASNLRAQTLHL
HHHHHHHHCCHHHHHHHHHCCCCHHHHHHHHHHHHCCEECCCCHHHHHHHHCCHHEEEEC
TDEDMAQIAALERNGREVSPDGLAPQWD
CHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796; 11108008 [H]