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The map label for this gene is narK [H]

Identifier: 187929590

GI number: 187929590

Start: 2627357

End: 2628643

Strand: Reverse

Name: narK [H]

Synonym: Rpic_2515

Alternate gene names: 187929590

Gene position: 2628643-2627357 (Counterclockwise)

Preceding gene: 187929591

Following gene: 187929575

Centisome position: 66.67

GC content: 63.95

Gene sequence:

>1287_bases
ATGCGAGCACCAGGCGCAACGAACAATCAGGCCGGCGCCCCGGCCATTTCGGCCCAAGCGTGGTCAGTGCTGCTGGCAAG
CACGCTCGCGTTCATGGTCTGCTTTGTGGTGTGGATGATGTTTGGCGTGATCGGCGTCCAGTTGCGCGAAGATCTGAAGC
TGAACGGCACCGAGTTCGGGTTGCTGACCTCCACGCCTGTGCTGACCGGAGCCCTGATGCGGCTGCCCCTGGGCGCGTGG
ACGGATCGTTTTGGCGGCCGCATCGTCATGACCACACTGCTTGTCGTCTGCGCGGTGCCGGTGTACGCCGTCAGCTACGC
GACCGCGCTTTGGCAGTTTCTGGTGATCGGCCTGTTTCTCGGCTGCGTCGGTGCGTCCTTCGCGGTCGGAACCCCCTACG
TTGCGCGCTTCTTTCCGCAGGAGCGACGCGGTTTTGCGATGGGGTTCTTTGGCGCCGGTACGTCGGGCGCGGCCATCAAC
CTTTTTCTCACGCCGTCGCTGCAGGCGGCTTACGGCTGGCGCTTCGTGCCGCGTGCGTATGCGGTTGCCCTGCTGGTGAC
GGCGGTCATCTTCTGGCTGGCATCGGCGTCCGACCCGGGCGCGGGGCGGGCGCAGGGGTCCTGGGTGGACAGTTTCAAGG
TGCTGCGTGACCGGCGCGTCTGGAGACTGTGCCAGTACTACTCGATCACCTTTGGCGGGTTCACCGCCCTGTCGTTGTGG
ATGCCGCAGTATCTGAAGAATGAGTATGGGATGACGCTGGTTGCGGCGTCGGCGTTTGCGGCGGGGTTTTCGTTGCCTGG
GTCGGTCCTGAGGGCCGTCGGTGGCTGGCTTGCCGATCGCTGGGGCGCCCAGAACGTGACCTGGTGGGGGCTGTGGGTCG
CGTGGGTCTGCCTGTTCTTGCTTGCGTATCCGCCGACCGACCTCGCGATCCACACCATCGACGGGGCATTCAATCTGCAT
CTGCAGATACCGTTGGCGGCATTCATTGCGCTGATCTTCCTGATGGGCAGCGTATTCGCCTTCGGCATGGCGTCTACCTT
CAAGTACGTGGCGGACGATTTCTCAGCAAACATGGGGGTCGTGACCGGTATCGTCGGCCTGGCCGGCGGCCTGGGCGGGT
TTCTCCTCCCCATCATCTTTGGCGTCTTGTTGGACGTGTTCCGAGTGCGCTCGTCGTGCTTCATGTTCCTCTACGGCATC
GTGTGGGTGTCGCTGATCCTCCTCTACCTGTCGGAAGTGCGGCGGGCACCGCTGACGGGGCACGCTGGGCAGGGGGCTGT
CCAATAA

Upstream 100 bases:

>100_bases
ATACTGGCTTCGCAGGCGCAAGTCGAAGGTGTGGATCTTGGGGCCGCCAACGGACCCCTGGTTCAATAGGTCGGCAACGC
ACAGAGGAAGGGGAGCCAGC

Downstream 100 bases:

>100_bases
GGTTGGGTGTTCGGAACAGGACCGCGAAAGGCGGCTTCCGCTTGGCCCATTGGCCTATTGGCGCTGCGGAAGATAGAGGG
TGACGAGCACCGACGCGCTT

Product: major facilitator superfamily protein

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 428; Mature: 428

Protein sequence:

>428_residues
MRAPGATNNQAGAPAISAQAWSVLLASTLAFMVCFVVWMMFGVIGVQLREDLKLNGTEFGLLTSTPVLTGALMRLPLGAW
TDRFGGRIVMTTLLVVCAVPVYAVSYATALWQFLVIGLFLGCVGASFAVGTPYVARFFPQERRGFAMGFFGAGTSGAAIN
LFLTPSLQAAYGWRFVPRAYAVALLVTAVIFWLASASDPGAGRAQGSWVDSFKVLRDRRVWRLCQYYSITFGGFTALSLW
MPQYLKNEYGMTLVAASAFAAGFSLPGSVLRAVGGWLADRWGAQNVTWWGLWVAWVCLFLLAYPPTDLAIHTIDGAFNLH
LQIPLAAFIALIFLMGSVFAFGMASTFKYVADDFSANMGVVTGIVGLAGGLGGFLLPIIFGVLLDVFRVRSSCFMFLYGI
VWVSLILLYLSEVRRAPLTGHAGQGAVQ

Sequences:

>Translated_428_residues
MRAPGATNNQAGAPAISAQAWSVLLASTLAFMVCFVVWMMFGVIGVQLREDLKLNGTEFGLLTSTPVLTGALMRLPLGAW
TDRFGGRIVMTTLLVVCAVPVYAVSYATALWQFLVIGLFLGCVGASFAVGTPYVARFFPQERRGFAMGFFGAGTSGAAIN
LFLTPSLQAAYGWRFVPRAYAVALLVTAVIFWLASASDPGAGRAQGSWVDSFKVLRDRRVWRLCQYYSITFGGFTALSLW
MPQYLKNEYGMTLVAASAFAAGFSLPGSVLRAVGGWLADRWGAQNVTWWGLWVAWVCLFLLAYPPTDLAIHTIDGAFNLH
LQIPLAAFIALIFLMGSVFAFGMASTFKYVADDFSANMGVVTGIVGLAGGLGGFLLPIIFGVLLDVFRVRSSCFMFLYGI
VWVSLILLYLSEVRRAPLTGHAGQGAVQ
>Mature_428_residues
MRAPGATNNQAGAPAISAQAWSVLLASTLAFMVCFVVWMMFGVIGVQLREDLKLNGTEFGLLTSTPVLTGALMRLPLGAW
TDRFGGRIVMTTLLVVCAVPVYAVSYATALWQFLVIGLFLGCVGASFAVGTPYVARFFPQERRGFAMGFFGAGTSGAAIN
LFLTPSLQAAYGWRFVPRAYAVALLVTAVIFWLASASDPGAGRAQGSWVDSFKVLRDRRVWRLCQYYSITFGGFTALSLW
MPQYLKNEYGMTLVAASAFAAGFSLPGSVLRAVGGWLADRWGAQNVTWWGLWVAWVCLFLLAYPPTDLAIHTIDGAFNLH
LQIPLAAFIALIFLMGSVFAFGMASTFKYVADDFSANMGVVTGIVGLAGGLGGFLLPIIFGVLLDVFRVRSSCFMFLYGI
VWVSLILLYLSEVRRAPLTGHAGQGAVQ

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=394, Percent_Identity=25.8883248730964, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787743, Length=406, Percent_Identity=26.3546798029557, Blast_Score=69, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46181; Mature: 46181

Theoretical pI: Translated: 9.48; Mature: 9.48

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCC
>Mature Secondary Structure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CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]