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The map label for this gene is rph

Identifier: 187929359

GI number: 187929359

Start: 2400367

End: 2401083

Strand: Direct

Name: rph

Synonym: Rpic_2280

Alternate gene names: 187929359

Gene position: 2400367-2401083 (Clockwise)

Preceding gene: 187929358

Following gene: 187929360

Centisome position: 60.88

GC content: 67.5

Gene sequence:

>717_bases
ATGCGACCCAGCGGCCGCCAGCCCGACCAACTCCGCCCTGTCACCCTCACCCGCAAGTTCACGCGCCATGCCGAAGGCTC
GGTGCTGGTGTGCTTTGGCGACACGCAAGTGCTCTGCACGGCCAGCGTGCTGCCCAAGGTGCCGCCGCACAAGAAGGGCA
GCGGCGAGGGCTGGGTGACAGCCGAATACGGCATGCTGCCGCGCTCGACGCACACGCGCTCCGACCGCGAGGCCGCACGC
GGCAAGCAGAGCGGCCGCACGCAGGAAATCCAGCGCCTGATCGGCCGCGCGATGCGTACAGTGTTCGATCTGCGCGCGCT
GGGCGAGCACACGCTGCATCTCGACTGCGACGTGCTGCAGGCCGATGGCGGCACGCGCACGGCCAGCATTACCGGCGCGT
TCGTCGCGGCATACGACGCTGTCTCGGTCATGCGCGAAAAGGGCCAGTTGGTGGGCGACCCGGTCCGCGACTTCGTGGCA
GCCGTATCGGTCGGGGTGGTCGACGGCGTACCGGTGCTCGATCTGGACTACCCCGAAGACTCCGCCTGCGATACCGACAT
GAACGTCGTCATGACCGGCAGTGGCGGCTTTGTGGAAGTGCAAGGCACCGCGGAAGGCACGCCGTTCTCGCGTGCCGAAA
TGGATGCAATGCTGGGCTTGGCCGATCAAGGCATCCGCACGCTGATCAGCCTGCAGAAGCAGGCGCTGGGCCTGTGA

Upstream 100 bases:

>100_bases
CTAAGCGCCACAGCGCAGACCCCCTCCTCCGGCCCGCCCTGCCAAGCGCAGCGCGGGCCGGTATCATGTTGGGCTATCCG
TTTCTTAAGCGAGACCCCAC

Downstream 100 bases:

>100_bases
TCGACACGGCCATGCGCAAGATCGTTCTCGCATCAAACAACGCCGGCAAGCTGGCCGAGTTCAACACGCTGCTGGGCACC
CTCGGGTTCGACGTGACGCC

Product: ribonuclease PH

Products: NA

Alternate protein names: RNase PH; tRNA nucleotidyltransferase

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MRPSGRQPDQLRPVTLTRKFTRHAEGSVLVCFGDTQVLCTASVLPKVPPHKKGSGEGWVTAEYGMLPRSTHTRSDREAAR
GKQSGRTQEIQRLIGRAMRTVFDLRALGEHTLHLDCDVLQADGGTRTASITGAFVAAYDAVSVMREKGQLVGDPVRDFVA
AVSVGVVDGVPVLDLDYPEDSACDTDMNVVMTGSGGFVEVQGTAEGTPFSRAEMDAMLGLADQGIRTLISLQKQALGL

Sequences:

>Translated_238_residues
MRPSGRQPDQLRPVTLTRKFTRHAEGSVLVCFGDTQVLCTASVLPKVPPHKKGSGEGWVTAEYGMLPRSTHTRSDREAAR
GKQSGRTQEIQRLIGRAMRTVFDLRALGEHTLHLDCDVLQADGGTRTASITGAFVAAYDAVSVMREKGQLVGDPVRDFVA
AVSVGVVDGVPVLDLDYPEDSACDTDMNVVMTGSGGFVEVQGTAEGTPFSRAEMDAMLGLADQGIRTLISLQKQALGL
>Mature_238_residues
MRPSGRQPDQLRPVTLTRKFTRHAEGSVLVCFGDTQVLCTASVLPKVPPHKKGSGEGWVTAEYGMLPRSTHTRSDREAAR
GKQSGRTQEIQRLIGRAMRTVFDLRALGEHTLHLDCDVLQADGGTRTASITGAFVAAYDAVSVMREKGQLVGDPVRDFVA
AVSVGVVDGVPVLDLDYPEDSACDTDMNVVMTGSGGFVEVQGTAEGTPFSRAEMDAMLGLADQGIRTLISLQKQALGL

Specific function: Phosphorolytic exoribonuclease that removes nucleotide residues following the -CCA terminus of tRNA and adds nucleotides to the ends of RNA molecules by using nucleoside diphosphates as substrates

COG id: COG0689

COG function: function code J; RNase PH

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase PH family

Homologues:

Organism=Escherichia coli, GI157672248, Length=210, Percent_Identity=68.0952380952381, Blast_Score=293, Evalue=6e-81,
Organism=Caenorhabditis elegans, GI71981632, Length=186, Percent_Identity=28.494623655914, Blast_Score=77, Evalue=6e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNPH_RALPJ (B2U8D8)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899846.1
- ProteinModelPortal:   B2U8D8
- SMR:   B2U8D8
- GeneID:   6287155
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2280
- HOGENOM:   HBG737187
- OMA:   MLPRATG
- ProtClustDB:   PRK00173
- HAMAP:   MF_00564
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR020568
- InterPro:   IPR002381
- InterPro:   IPR018336
- TIGRFAMs:   TIGR01966

Pfam domain/function: PF01138 RNase_PH; PF03725 RNase_PH_C; SSF55666 3_ExoRNase; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.56

Molecular weight: Translated: 25485; Mature: 25485

Theoretical pI: Translated: 6.36; Mature: 6.36

Prosite motif: PS01277 RIBONUCLEASE_PH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPSGRQPDQLRPVTLTRKFTRHAEGSVLVCFGDTQVLCTASVLPKVPPHKKGSGEGWVT
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEHHHCCCCCCCCCCCCCCEEE
AEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRAMRTVFDLRALGEHTLHLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
ADGGTRTASITGAFVAAYDAVSVMREKGQLVGDPVRDFVAAVSVGVVDGVPVLDLDYPED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC
SACDTDMNVVMTGSGGFVEVQGTAEGTPFSRAEMDAMLGLADQGIRTLISLQKQALGL
CCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRPSGRQPDQLRPVTLTRKFTRHAEGSVLVCFGDTQVLCTASVLPKVPPHKKGSGEGWVT
CCCCCCCCCCCCCCHHHHHHHHCCCCCEEEEECCCEEEEEHHHCCCCCCCCCCCCCCEEE
AEYGMLPRSTHTRSDREAARGKQSGRTQEIQRLIGRAMRTVFDLRALGEHTLHLDCDVLQ
EECCCCCCCCCCCCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEE
ADGGTRTASITGAFVAAYDAVSVMREKGQLVGDPVRDFVAAVSVGVVDGVPVLDLDYPED
CCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCC
SACDTDMNVVMTGSGGFVEVQGTAEGTPFSRAEMDAMLGLADQGIRTLISLQKQALGL
CCCCCCCEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA