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The map label for this gene is spoT [H]

Identifier: 187929353

GI number: 187929353

Start: 2393424

End: 2395844

Strand: Reverse

Name: spoT [H]

Synonym: Rpic_2274

Alternate gene names: 187929353

Gene position: 2395844-2393424 (Counterclockwise)

Preceding gene: 187929354

Following gene: 187929352

Centisome position: 60.77

GC content: 63.28

Gene sequence:

>2421_bases
ATGCCGTCATCCGCTTCCGGCTCCGGCCCCGCGTCGCCTCGTACGGGTGCGCCGAACTTGCCTGATCCGCGTGGCGCCGA
CCTGGTAGGGGAGCGGGCAGCCCTGCCGCCCAAATCGCATAAGGGCGCAAGCGCCGACGCCGCTGGCTCGACAGCCAAGC
CGGAGGGCGCCGACACGCTCTTCATTGATGCGGTGCTCGAGCAGACCTATCGGCATCTTTTCGGCCCGACCTCGCAGCCG
GCGGCACCGCCTCGCCAACAGGTCGTCTCCATTTCGGGGCTGACCGAAAAGCTGTCGTATCTGAAGCCGGCGGATCTCAA
GCTGGTGAAGGAAGCCTTCCACTTTTCCGATGAGGCGCACCTCGGGCAGTACCGCCAAAGCGGCGAGCCGTACATCACGC
ATCCCGTGGCCGTGGCAGAGATCTGCGCGGGCTGGAAGCTGGATGTGCAATCGATCATGGCGGCGCTGCTGCACGACGTG
ATCGAAGACCAGGGCGTGACCAAGAGCGAGCTTGCCGAGAAGTTCGGCCCCAAAGTCGCAGAGCTGGTCGACGGCCTGAC
CAAGTTGGACAAGCTTGAATTCCAGAGCCGCGAACAGGCGCAAGCCGAGAGCTTCCGCAAGATGCTGCTCGCGATGGCGC
GCGATGTGCGCGTGATTCTCGTCAAGCTCGCCGATCGTACGCACAACATGCGCACGCTCGACCACGTGCCGCCGGAGAAG
CGCCGCCGCATTGCCGGCGAGACGATGGAGATTTACGCGCCGATCGCGCACCGCCTCGGCCTGAATACGACATACCGGGA
GCTGCAGGAACTGAGCTTCCGTATCGGCTCGCCGTTCCGGTATGCCACGCTCGAGAAGGCGGTGAAGGCGGCGCGCGGCA
ATCGGCGCGAGGTCGTGAGCCGCATTCTGGAAACGGCCCAGCGCGCGCTGACCGACGCGGGCATCCCGGCCGAAATGACC
GGGCGCGAAAAAACCCTGTACAGCATTTACCGCAAGATGCACGACAAGCAGTTGTCGTTCTCGCAGGTGCTGGATGTGTA
TGGTTTTCGCGTGGTGGTCGACACGCAGATGCAGTGCTACATGACGATGGGTGCGCTGCACAGCCTGTACAAGCCGATGC
CCGGCAAGTTCAAAGACTACATTGCCATCCCCAAGATCAACGGTTATCAGTCGCTGCACACGACGCTGGTGGGCCCGTTC
GGCACACCGGTCGAGTTCCAGATCCGGACGCGTGAGATGAACCAGATTGCGGAAGCCGGCGTGGCCGCACACTGGATGTA
CAAGCAGCACCACGACGAGCCCGATCGCGCGCAGCAGCAAGCGCATCAGTGGCTGCAGTCGCTGCTCGACATCCAAAGCC
AGACGGGGGATTCGCAGGAGTTTCTGGAGCACGTCAAGATCGACCTGTTCCCGGATGCGGTCTACGTGTTCACGCCCAAG
GGCGAGATCCGCGCGCTGCCGCGCGGTGCTACGGCGCTGGACTTCGCCTACGCTGTGCACAGCGATCTGGGCAACCAGTG
TGTGGCGGTCAAGATCAACAACGAACTGCTGCCGTTGCGTACGGAGCTGAAAAACGGCGACATCGTCGAGGTGGTGACGG
CGCCGTATTCAAAGCCGAACCCCGCATGGCTCACGTTCGTGCGCACAGGCAAGGCGCGCGCGGCGATCCGGCATTTCCTC
AAGACGGCCAAGCTGGATGAGGCGATTCAACTGGGCGAGCGTTTGCTCGAACAGGCGCTGCGTCAGATCGGCATCGACAT
GAAGGCCGTGTCCGCCCAGGTATGGGAGCGCGTCGTGCAGTGGACGGGCAACAAGGCGCGTGAAGACGTTTTTGCGGATC
TCGCGCTCGGCCGCCGGGTGGCCGCCGTGGTTGCGCGCCGTCTCGAAATTCTTCTGCAAGAAGGCGGTCACGACGGCGAT
GAAGCCTTGATGGCGGCCGTGCATACCTTCGCTGGCGACGAGGCCCCTGCCGTCACCGTGAGTGGCGACGAAGGCATGGC
AATGGTGTTCTCTTCGTGCTGCCGCCCGATTCCCGGTGACCCGATCGTCGGCTATATCGGCAAGGGCGAAGGCTTGCAGA
TCCACGTGCAGGAATGCCGCGTGGCCAAACGTCTGCATGGCAAGGATCCCGAGCATTGGATTGACGTCATGTGGGCGGAG
CACACGACACGCGCGTTCGATGTGTCGATCAAGGTGCTGGTGCGCAATACGAAGGGCATTCTGGCGCGTGTCGCCGCCGA
CCTGACGTCGGCTGACGCCAACGTGGCGCACGTGTCGATGGAGCAAGAGGGCGATCAGGAAGCGACGTACATGACGTTCC
TGATCCAGGTGCATGATCGCGTGCACTTGGCCGATGTGATGCGCGCGCTGCGGCGCAACCCGGATGTGATTCGTATTGCG
CGAGATCGCGGTGGAGATTGA

Upstream 100 bases:

>100_bases
ACACAACGTGCCGTCGGTGGAATCGTCCGCGAGGTCGTCCGGTCCTGAGCCGGGCGCTTCCACGGTCCCTTCCGGGTCTT
CCACCGCTTCCATCGTTTCC

Downstream 100 bases:

>100_bases
CGCTCCGAGCGGAAACAAAAAAAGGCGAGGCCACATGGCTTCGCCTTTTTTGGTTGTGGCCTGCTAGAGCACACACAAGA
GCGCTTTGGCACGACCGGCG

Product: (p)ppGpp synthetase I SpoT/RelA

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 806; Mature: 805

Protein sequence:

>806_residues
MPSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAAGSTAKPEGADTLFIDAVLEQTYRHLFGPTSQP
AAPPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDV
IEDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEK
RRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILETAQRALTDAGIPAEMT
GREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCYMTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPF
GTPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPK
GEIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKARAAIRHFL
KTAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWERVVQWTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHDGD
EALMAAVHTFAGDEAPAVTVSGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAE
HTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDRVHLADVMRALRRNPDVIRIA
RDRGGD

Sequences:

>Translated_806_residues
MPSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAAGSTAKPEGADTLFIDAVLEQTYRHLFGPTSQP
AAPPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDV
IEDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEK
RRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILETAQRALTDAGIPAEMT
GREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCYMTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPF
GTPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPK
GEIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKARAAIRHFL
KTAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWERVVQWTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHDGD
EALMAAVHTFAGDEAPAVTVSGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAE
HTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDRVHLADVMRALRRNPDVIRIA
RDRGGD
>Mature_805_residues
PSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAAGSTAKPEGADTLFIDAVLEQTYRHLFGPTSQPA
APPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAHLGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVI
EDQGVTKSELAEKFGPKVAELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEKR
RRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVSRILETAQRALTDAGIPAEMTG
REKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCYMTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFG
TPVEFQIRTREMNQIAEAGVAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPKG
EIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPNPAWLTFVRTGKARAAIRHFLK
TAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWERVVQWTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHDGDE
ALMAAVHTFAGDEAPAVTVSGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAEH
TTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDRVHLADVMRALRRNPDVIRIAR
DRGGD

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Escherichia coli, GI1790082, Length=707, Percent_Identity=47.3833097595474, Blast_Score=678, Evalue=0.0,
Organism=Escherichia coli, GI1789147, Length=696, Percent_Identity=31.7528735632184, Blast_Score=382, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI25143118, Length=145, Percent_Identity=33.1034482758621, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 89293; Mature: 89162

Theoretical pI: Translated: 7.35; Mature: 7.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAAGSTAKPEGADTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
FIDAVLEQTYRHLFGPTSQPAAPPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
LGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGPKVA
HHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
ELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVS
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
RILETAQRALTDAGIPAEMTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECHHHHH
MTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTREMNQIAEAG
HHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHH
VAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCEEEEECCC
GEIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPN
CCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEECCCCCCC
PAWLTFVRTGKARAAIRHFLKTAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWERVVQ
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHDGDEALMAAVHTFAGDEAPAVTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEE
SGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAE
ECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHC
HTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDR
CCCEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
VHLADVMRALRRNPDVIRIARDRGGD
HHHHHHHHHHHCCCCEEEEECCCCCC
>Mature Secondary Structure 
PSSASGSGPASPRTGAPNLPDPRGADLVGERAALPPKSHKGASADAAGSTAKPEGADTL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHH
FIDAVLEQTYRHLFGPTSQPAAPPRQQVVSISGLTEKLSYLKPADLKLVKEAFHFSDEAH
HHHHHHHHHHHHHCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCHHHHHHHHHHCCCHHH
LGQYRQSGEPYITHPVAVAEICAGWKLDVQSIMAALLHDVIEDQGVTKSELAEKFGPKVA
HHHHHHCCCCEEECHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCHHH
ELVDGLTKLDKLEFQSREQAQAESFRKMLLAMARDVRVILVKLADRTHNMRTLDHVPPEK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
RRRIAGETMEIYAPIAHRLGLNTTYRELQELSFRIGSPFRYATLEKAVKAARGNRREVVS
HHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHH
RILETAQRALTDAGIPAEMTGREKTLYSIYRKMHDKQLSFSQVLDVYGFRVVVDTQMQCY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCEEEEECHHHHH
MTMGALHSLYKPMPGKFKDYIAIPKINGYQSLHTTLVGPFGTPVEFQIRTREMNQIAEAG
HHHHHHHHHHCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCEEEEEHHHHHHHHHHHH
VAAHWMYKQHHDEPDRAQQQAHQWLQSLLDIQSQTGDSQEFLEHVKIDLFPDAVYVFTPK
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCEEECCEEEEECCC
GEIRALPRGATALDFAYAVHSDLGNQCVAVKINNELLPLRTELKNGDIVEVVTAPYSKPN
CCEEECCCCCHHHHHHHHHHHCCCCCEEEEEECCCEECHHHHCCCCCEEEEEECCCCCCC
PAWLTFVRTGKARAAIRHFLKTAKLDEAIQLGERLLEQALRQIGIDMKAVSAQVWERVVQ
CCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
WTGNKAREDVFADLALGRRVAAVVARRLEILLQEGGHDGDEALMAAVHTFAGDEAPAVTV
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCCEEEE
SGDEGMAMVFSSCCRPIPGDPIVGYIGKGEGLQIHVQECRVAKRLHGKDPEHWIDVMWAE
ECCCCHHHHHHHHCCCCCCCCEEEEECCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHC
HTTRAFDVSIKVLVRNTKGILARVAADLTSADANVAHVSMEQEGDQEATYMTFLIQVHDR
CCCEEEEEEEEEEEECHHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHCC
VHLADVMRALRRNPDVIRIARDRGGD
HHHHHHHHHHHCCCCEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]