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The map label for this gene is ilvI [H]

Identifier: 187929307

GI number: 187929307

Start: 2338530

End: 2340287

Strand: Reverse

Name: ilvI [H]

Synonym: Rpic_2228

Alternate gene names: 187929307

Gene position: 2340287-2338530 (Counterclockwise)

Preceding gene: 187929319

Following gene: 187929306

Centisome position: 59.36

GC content: 60.86

Gene sequence:

>1758_bases
ATGAATATGCCAAGCGCGGAATTTTCCCACGCCAATAGCGGTTCACCTGCCGACATGATGGGGGCTGACATCCTCGTCAA
TGCGCTCGCGGCAGAGGGCGTCGAGTTCGTCTGGGGTTACCCCGGCGGCGCGGTGCTCTATATCTACGACGCGTTCTACA
AGCAAAACAAGATCGAACACATCCTGGTGCGCCATGAACAGGCGGCAGTCCATGCGGCTGACGGCTATGCGCGTGCCACG
GGCAAGGTCGGTGTCGCCCTTGTGACGTCCGGCCCGGGCGTGACCAATGCCGTGACAGGCATCGCCACTGCTTACCTGGA
TTCGATCCCGATGGTGATCATCACCGGCAACGTGCCGACGCACGCCATCGGCCAGGACGCCTTCCAGGAGTGCGATACCG
TCGGCATTACCCGCCCGATCGTCAAGCACAACTTCCTGGTCAAGGACGTGCGCAACCTCGCCTCGACCATCAAGAAGGCG
TTCTACATCGCCGCAACGGGCCGTCCGGGTCCGGTGGTGGTGGATATCCCCAAGGACGTCTCGCGCGAACTCTGCAAGTA
CGAGTACCCGAAGACCATCGAGATGCGCTCGTACAGCCCGGTCAACAAGGGGCATTCGGGGCAGATTCGGAAGGCAGTGA
GCCTGCTGCTGCAGGCCGAGCGTCCGTACATCTATTCGGGTGGCGGCGTGGTGCTTGCCGAGGCCAGCGAAGAGCTGCGC
CAGCTGGCTGCGCTGACGGGCTACCCCGTGACGAACACGCTGATGGGCCTGGGTGCGTTCCCCGGCACCAGCAAGCAGTT
CGTGGGCATGCTCGGCATGCACGGCACGTACGAAGCCAACATGGCGATGCAGAACTGCGACGTTCTGATTGCCATCGGCG
CGCGATTCGATGATCGCGTGATCGGCAGCCCGGCGCACTTCACCTCGCAGCCGCGCAAGATCATCCACATCGACATCGAT
CCGTCCTCCATTTCGAAGCGGGTGAAGGTCGACATCCCCATCGTCGGCAACGTCAAGGACGTGCTGCAGGAGATGATTGC
CCAGATCCAGTCTGGCGAAGCCAAGCCGAACAAGACGGCGCTGGCCAAGTGGTGGGAGCAGATCGAGCAGTGGCGCAGTG
TCGATTGCCTGAAATACGACCGCACCTCCGAGATCATCAAGCCGCAGTATGTGGTCGAGAAGATCTGGGAACTGACCAAT
GGCGATGCATTCATCTGCTCCGACGTCGGTCAGCATCAGATGTGGGCTGCGCAGTTCTACAAGTTCAACGAGCCGCGTCG
CTGGATCAACTCTGGCGGCCTGGGCACGATGGGCGTGGGGCTGCCGTACGCGATGGGCATCAAGAAAGCGTTCCCGGACA
AGGAGGTCGTCACCATCACCGGTGAAGGCTCGATCCAGATGTGTATCCAGGAACTGTCGACGTGCCTGCAGTACGACACG
CCGGTGAAGATCTGCTCGCTCAACAACGGTTATCTGGGCATGGTGCGCCAGTGGCAGGAAATCGAATACGACAACCGCTA
CTCGCATTCCTACATGCAGGCGCTGCCGGACTTCGTGAAGTTGGCTGAATCGTATGGCCACGTCGGCATGCGCATCGAGA
AGACGTCTGACGTCGAGCCCGCGCTGCGCGAAGCCTTCCGACTCAAGGATCGCACCGTGTTCCTCGACTTCCAGACCGAC
CCGACAGAAAACGTCTGGCCGATGGTTCAGGCAGGCAAGGGTATTTCTGAAATGCTGCTCGGCGCGGAGGACCTCTAA

Upstream 100 bases:

>100_bases
AGGCGGACCATCCATGCAGACGCGCACCGCTCAAATCCGCGCCACAAGCCCGGTAGAGAGCATCCAAACAATTTTTTGCC
GAAAATTGCAAAGGACTGAC

Downstream 100 bases:

>100_bases
TGCGCCACATCATTTCCGTCCTGCTGGAAAACGAACCGGGCGCGCTGTCGCGCGTGGTGGGCCTGTTCTCGGCGCGCGGC
TACAACATCGAGACGCTGAC

Product: acetolactate synthase 3 catalytic subunit

Products: NA

Alternate protein names: AHAS; Acetohydroxy-acid synthase large subunit; ALS [H]

Number of amino acids: Translated: 585; Mature: 585

Protein sequence:

>585_residues
MNMPSAEFSHANSGSPADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIAATGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYSPVNKGHSGQIRKAVSLLLQAERPYIYSGGGVVLAEASEELR
QLAALTGYPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGSPAHFTSQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQSGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTN
GDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAESYGHVGMRIEKTSDVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL

Sequences:

>Translated_585_residues
MNMPSAEFSHANSGSPADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIAATGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYSPVNKGHSGQIRKAVSLLLQAERPYIYSGGGVVLAEASEELR
QLAALTGYPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGSPAHFTSQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQSGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTN
GDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAESYGHVGMRIEKTSDVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL
>Mature_585_residues
MNMPSAEFSHANSGSPADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEHILVRHEQAAVHAADGYARAT
GKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPTHAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKA
FYIAATGRPGPVVVDIPKDVSRELCKYEYPKTIEMRSYSPVNKGHSGQIRKAVSLLLQAERPYIYSGGGVVLAEASEELR
QLAALTGYPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRVIGSPAHFTSQPRKIIHIDID
PSSISKRVKVDIPIVGNVKDVLQEMIAQIQSGEAKPNKTALAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTN
GDAFICSDVGQHQMWAAQFYKFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAESYGHVGMRIEKTSDVEPALREAFRLKDRTVFLDFQTD
PTENVWPMVQAGKGISEMLLGAEDL

Specific function: Valine and isoleucine biosynthesis; first step. [C]

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=581, Percent_Identity=25.3012048192771, Blast_Score=172, Evalue=1e-42,
Organism=Homo sapiens, GI21361361, Length=512, Percent_Identity=26.5625, Blast_Score=155, Evalue=1e-37,
Organism=Escherichia coli, GI87081685, Length=566, Percent_Identity=56.1837455830389, Blast_Score=675, Evalue=0.0,
Organism=Escherichia coli, GI1790104, Length=559, Percent_Identity=39.7137745974955, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1786717, Length=541, Percent_Identity=34.7504621072089, Blast_Score=309, Evalue=4e-85,
Organism=Escherichia coli, GI1787096, Length=568, Percent_Identity=28.6971830985916, Blast_Score=205, Evalue=5e-54,
Organism=Escherichia coli, GI1788716, Length=553, Percent_Identity=25.1356238698011, Blast_Score=150, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17531299, Length=550, Percent_Identity=26.1818181818182, Blast_Score=162, Evalue=7e-40,
Organism=Caenorhabditis elegans, GI17531301, Length=550, Percent_Identity=26.1818181818182, Blast_Score=161, Evalue=8e-40,
Organism=Caenorhabditis elegans, GI17542570, Length=566, Percent_Identity=25.4416961130742, Blast_Score=147, Evalue=1e-35,
Organism=Saccharomyces cerevisiae, GI6323755, Length=582, Percent_Identity=41.7525773195876, Blast_Score=441, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6320816, Length=495, Percent_Identity=24.040404040404, Blast_Score=111, Evalue=4e-25,
Organism=Saccharomyces cerevisiae, GI6323163, Length=518, Percent_Identity=23.5521235521236, Blast_Score=78, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320123, Length=511, Percent_Identity=21.3307240704501, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=25.9965337954939, Blast_Score=178, Evalue=7e-45,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012846
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =2.2.1.6 [H]

Molecular weight: Translated: 64467; Mature: 64467

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNMPSAEFSHANSGSPADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHHCCCHHH
ILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPT
HEEECHHHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
HAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKAFYIAATGRPGPVVVDIPKDV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCHHH
SRELCKYEYPKTIEMRSYSPVNKGHSGQIRKAVSLLLQAERPYIYSGGGVVLAEASEELR
HHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QLAALTGYPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRV
HHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCCCC
IGSPAHFTSQPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQEMIAQIQSGEAKPNKTA
CCCCCCCCCCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHH
LAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTNGDAFICSDVGQHQMWAAQFY
HHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
KFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAESYGHVGMRIEKTSDVEP
CEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
ALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC
>Mature Secondary Structure
MNMPSAEFSHANSGSPADMMGADILVNALAAEGVEFVWGYPGGAVLYIYDAFYKQNKIEH
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCEEEEEEEHHHHCCCHHH
ILVRHEQAAVHAADGYARATGKVGVALVTSGPGVTNAVTGIATAYLDSIPMVIITGNVPT
HEEECHHHHEEECCCCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHCCCCEEEEECCCCC
HAIGQDAFQECDTVGITRPIVKHNFLVKDVRNLASTIKKAFYIAATGRPGPVVVDIPKDV
CCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCEEEECCHHH
SRELCKYEYPKTIEMRSYSPVNKGHSGQIRKAVSLLLQAERPYIYSGGGVVLAEASEELR
HHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCEEEECCCEEEECCHHHHH
QLAALTGYPVTNTLMGLGAFPGTSKQFVGMLGMHGTYEANMAMQNCDVLIAIGARFDDRV
HHHHHCCCCHHHHHHHCCCCCCCCHHHHHHHCCCCCEECCCHHCCCCEEEEECCCCCCCC
IGSPAHFTSQPRKIIHIDIDPSSISKRVKVDIPIVGNVKDVLQEMIAQIQSGEAKPNKTA
CCCCCCCCCCCCEEEEEEECHHHHCCEEEEEEEEECCHHHHHHHHHHHHHCCCCCCCHHH
LAKWWEQIEQWRSVDCLKYDRTSEIIKPQYVVEKIWELTNGDAFICSDVGQHQMWAAQFY
HHHHHHHHHHHCCCCCEEECCCHHHCCHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHH
KFNEPRRWINSGGLGTMGVGLPYAMGIKKAFPDKEVVTITGEGSIQMCIQELSTCLQYDT
HCCCCHHHHCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHCCCC
PVKICSLNNGYLGMVRQWQEIEYDNRYSHSYMQALPDFVKLAESYGHVGMRIEKTSDVEP
CEEEEECCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCH
ALREAFRLKDRTVFLDFQTDPTENVWPMVQAGKGISEMLLGAEDL
HHHHHHHCCCCEEEEEECCCCCCCCCHHHHCCCCHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]