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The map label for this gene is pnp

Identifier: 187929298

GI number: 187929298

Start: 2328348

End: 2330522

Strand: Reverse

Name: pnp

Synonym: Rpic_2219

Alternate gene names: 187929298

Gene position: 2330522-2328348 (Counterclockwise)

Preceding gene: 187929299

Following gene: 187929297

Centisome position: 59.11

GC content: 62.67

Gene sequence:

>2175_bases
ATGACCATGTTCAATAAGGTCGTCAAAGAATTCCAATGGGGCGGCCACAAGGTCCGCATGGAAACGGGTGAGATCGCCCG
CCAGGCGAGCGGCGCTGTGCTGCTCGACATGGACGACACCGTCGTGCTCGCTACCGTGGTGGGCGCCAAGAACCCGAAGC
CGGGTCAGGACTTCTTCCCGCTGACTGTCGATTACCTCGAGAAGACCTACGCCGCTGGCAAGATCCCGGGTGGCTTCTTC
AAGCGCGAAGGCCGTCCGTCGGAAAACGAAACGCTGACCTCGCGCCTGATCGATCGCCCGCTGCGTCCGCTGTTCCCGGA
AGGCTTCTACAACGAAGTCCAGGTCGTTATCCACGTGCTGTCGATCAACCCGGAAGTGCCGGCTGACATCCCGGCGCTGG
TTGCTGCTTCGGCTGCACTGGCTGTGTCGGGTCTGCCGTTCAACGGCCCGGTGGGCGCTGCCCGCGTGGGTTACAAGGAT
GGCCAGTACCTGCTGAACCCGAACCGCGCACAACTGGCGCACTCGGATCTGGACCTGGTTGTTGCCGGTACCGAGCGTGC
CGTGCTGATGGTCGAATCCGAAGCGCAGCAGCTGTCGGAAGAGATCATGCTGGGTGCCGTGGTGTACGGCCATGAGCAGA
TGCAGATCGCCATCAACGCGATCCACGACCTGGTGCGCGACGGCGGCAAGCCTGAGTGGGATTGGCAAGCTGCGCCGAAG
AACGAAGCGTTGGTCGCAAAGGTGTCCGAACTGGGCCTGGCCGATCTGCAAGCTGCTTACCAGCTGCGCCAGAAGTCGGC
CCGCAGCCAGAAGTTGAAGGAAGTCTACAAGTCGGTTGCGGCCAAGCTGGCCGAAGCCGGCGTGGAAGCCGATGGCGTGG
AAGTCGACAACATCCTGTTCGAGCTCGAATCGAAGATCGTGCGCGGCCAGATCCTGAATGGCGAACCGCGTATCGACGGC
CGCGACACCCGCACGGTGCGCCCGATCGAGATCCGTTCGTCGGTGCTGCCGCGTGCGCACGGCTCGTCGCTGTTCACGCG
TGGTGAAACGCAAGCGCTGGTGGTGGCCGCTCTGGGCACCAAGAGCGACGAGCAGATCATCGACGCGCTGCAAGGCGAGT
ACCGTGATCGCTTCATGCTCCACTACAACATGCCGCCGTTCGCCACGGGCGAAACTGGCCGCGTGGGCAGCCCGAAGCGC
CGCGAAGTCGGCCATGGCCGTTTGGCCAAGCGCGCACTGATTCCGGTGCTGCCGCCCGCCGACGAATTCGGCTACACGAT
CCGTCTGGTGTCGGAAATCACCGAGTCGAATGGCTCGTCGTCGATGGCTTCGGTGTGCGGCGGCTCGCTGGCACTGATGG
ACGCTGGCGTGCCCATCAAAGCGCACGTTGCTGGCGTGGCCATGGGCCTGATCCTGGAAGACAACAAGTTCGCCGTGCTG
ACCGACATCCTGGGTGACGAAGATCACCTGGGTGACATGGACTTCAAGGTCGCTGGTACCGACGCTGGTATCACCGCACT
GCAGATGGACATCAAGGTGCAGGGCATCACCAAGGAAATCATGCAGGTGGCACTGGCGCAAGCCAAGGAAGGCCGTCTGC
ACATCCTGGGCAAGATGCAGGCTGCCATGGGCGGCGCACGCACCGAGCTGTCGGAGCACGCACCGCGCATGATCACGGTC
AAGATCAACCCGGAGAAGATCCGCGACGTGATTGGCAAGGGTGGTTCGACCATCCAGGCGCTGACCAAGGAAACCGGCTG
CACGATCGACATCGGGGAAGACGGCACGATCACCATCGCGTCGACCTCGTCGGAAGGCATGGCAGAAGCCAAGCGCCGCA
TCGAAGGCATCACGGCAGAAGCCGAAGTGGGCAAGATCTACAACGGTACCGTGCTCAAACTGCTGGACTTCGGCGCGATC
GTGAACATCCTGCCGGGCAAGGACGGTCTGCTGCACATCTCGGAAATCGCCAACGAGCGCGTGAACCAGGTTTCCGACTA
TGTGAAGGAAGGCCAGGCTGTGCGCGTGAAGCTGCTGAGCACCGATGAAAAGGGTCGCATGCGCCTGTCGATCAAGGCCG
CCAAGGCCGAAGAGGGCGACGTGCCGGTCGCTGCACCGCAAGCACCGGGTGCTGGCGACGCTGCCTCGCAACAACAGCAG
CAACAACAGCAGTAA

Upstream 100 bases:

>100_bases
ATGGCATAAAACACACCTCTGCAACCGCACCGTCTTACGGAACGTATTTGTCGCGGTGAAGCGCAATGAAGCGCGCCGCG
AATACCGAACAGGAGATGCT

Downstream 100 bases:

>100_bases
GCGAGCGATTTTCGCCAAGAAAAACGGCTGGACGATGTCCAGCCGTTTTTTTGTTTACACTGCGTCGCGAGTTCTTCCTG
TTGTCCCTTCGCGCCCCATC

Product: polynucleotide phosphorylase/polyadenylase

Products: NA

Alternate protein names: Polynucleotide phosphorylase; PNPase

Number of amino acids: Translated: 724; Mature: 723

Protein sequence:

>724_residues
MTMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFPLTVDYLEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKD
GQYLLNPNRAQLAHSDLDLVVAGTERAVLMVESEAQQLSEEIMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPK
NEALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSSLFTRGETQALVVAALGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKR
REVGHGRLAKRALIPVLPPADEFGYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPIKAHVAGVAMGLILEDNKFAVL
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGGARTELSEHAPRMITV
KINPEKIRDVIGKGGSTIQALTKETGCTIDIGEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAI
VNILPGKDGLLHISEIANERVNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPVAAPQAPGAGDAASQQQQ
QQQQ

Sequences:

>Translated_724_residues
MTMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFPLTVDYLEKTYAAGKIPGGFF
KREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKD
GQYLLNPNRAQLAHSDLDLVVAGTERAVLMVESEAQQLSEEIMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPK
NEALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDG
RDTRTVRPIEIRSSVLPRAHGSSLFTRGETQALVVAALGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKR
REVGHGRLAKRALIPVLPPADEFGYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPIKAHVAGVAMGLILEDNKFAVL
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGGARTELSEHAPRMITV
KINPEKIRDVIGKGGSTIQALTKETGCTIDIGEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAI
VNILPGKDGLLHISEIANERVNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPVAAPQAPGAGDAASQQQQ
QQQQ
>Mature_723_residues
TMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFPLTVDYLEKTYAAGKIPGGFFK
REGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVLSINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDG
QYLLNPNRAQLAHSDLDLVVAGTERAVLMVESEAQQLSEEIMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPKN
EALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVAAKLAEAGVEADGVEVDNILFELESKIVRGQILNGEPRIDGR
DTRTVRPIEIRSSVLPRAHGSSLFTRGETQALVVAALGTKSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRR
EVGHGRLAKRALIPVLPPADEFGYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPIKAHVAGVAMGLILEDNKFAVLT
DILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQAAMGGARTELSEHAPRMITVK
INPEKIRDVIGKGGSTIQALTKETGCTIDIGEDGTITIASTSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIV
NILPGKDGLLHISEIANERVNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPVAAPQAPGAGDAASQQQQQ
QQQ

Specific function: Involved in mRNA degradation. Hydrolyzes single-stranded polyribonucleotides processively in the 3'- to 5'-direction

COG id: COG1185

COG function: function code J; Polyribonucleotide nucleotidyltransferase (polynucleotide phosphorylase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain

Homologues:

Organism=Homo sapiens, GI188528628, Length=710, Percent_Identity=37.887323943662, Blast_Score=451, Evalue=1e-126,
Organism=Escherichia coli, GI145693187, Length=692, Percent_Identity=62.2832369942196, Blast_Score=889, Evalue=0.0,
Organism=Caenorhabditis elegans, GI115534063, Length=712, Percent_Identity=32.4438202247191, Blast_Score=319, Evalue=4e-87,
Organism=Drosophila melanogaster, GI281362905, Length=722, Percent_Identity=37.1191135734072, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651641, Length=722, Percent_Identity=37.1191135734072, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI24651643, Length=722, Percent_Identity=37.1191135734072, Blast_Score=436, Evalue=1e-122,
Organism=Drosophila melanogaster, GI161079377, Length=667, Percent_Identity=36.7316341829085, Blast_Score=393, Evalue=1e-109,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1000 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 3328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): PNP_RALPJ (B2U7R6)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899785.1
- ProteinModelPortal:   B2U7R6
- GeneID:   6289109
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2219
- HOGENOM:   HBG382411
- OMA:   YGETVVL
- ProtClustDB:   PRK11824
- GO:   GO:0005739
- HAMAP:   MF_01595
- InterPro:   IPR001247
- InterPro:   IPR015847
- InterPro:   IPR004087
- InterPro:   IPR004088
- InterPro:   IPR018111
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR012162
- InterPro:   IPR015848
- InterPro:   IPR003029
- InterPro:   IPR020568
- InterPro:   IPR022967
- Gene3D:   G3DSA:2.40.50.140
- Gene3D:   G3DSA:1.10.10.400
- PANTHER:   PTHR11252
- PIRSF:   PIRSF005499
- SMART:   SM00322
- SMART:   SM00316
- TIGRFAMs:   TIGR03591

Pfam domain/function: PF00013 KH_1; PF03726 PNPase; PF01138 RNase_PH; PF03725 RNase_PH_C; PF00575 S1; SSF46915 3_ExoRNase; SSF55666 3_ExoRNase; SSF50249 Nucleic_acid_OB; SSF54211 Ribosomal_S5_D2-typ_fold

EC number: =2.7.7.8

Molecular weight: Translated: 77773; Mature: 77642

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS50084 KH_TYPE_1; PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFP
CCHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCC
LTVDYLEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEE
SINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQYLLNPNRAQLAHSDLDLV
ECCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCEEE
VAGTERAVLMVESEAQQLSEEIMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPK
EECCCEEEEEECHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVAAKLAEAGVEADGVEVDNILF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ELESKIVRGQILNGEPRIDGRDTRTVRPIEIRSSVLPRAHGSSLFTRGETQALVVAALGT
HHHHHHHHCEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCCCEECCCCCEEEEEEECC
KSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRREVGHGRLAKRALIPVLPPA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCC
DEFGYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPIKAHVAGVAMGLILEDNKFAVL
CCCCHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQ
EECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHH
AAMGGARTELSEHAPRMITVKINPEKIRDVIGKGGSTIQALTKETGCTIDIGEDGTITIA
HHHCCHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEE
STSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIVNILPGKDGLLHISEIANER
ECCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCHHEEECCCCCCCEEHHHHHHHH
VNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPVAAPQAPGAGDAASQQQQ
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHH
QQQQ
HHCC
>Mature Secondary Structure 
TMFNKVVKEFQWGGHKVRMETGEIARQASGAVLLDMDDTVVLATVVGAKNPKPGQDFFP
CHHHHHHHHHHCCCEEEEEEHHHHHHHCCCEEEEECCCCEEEEEECCCCCCCCCCCCCC
LTVDYLEKTYAAGKIPGGFFKREGRPSENETLTSRLIDRPLRPLFPEGFYNEVQVVIHVL
CHHHHHHHHHHCCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHHEEEEE
SINPEVPADIPALVAASAALAVSGLPFNGPVGAARVGYKDGQYLLNPNRAQLAHSDLDLV
ECCCCCCCCCHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCEEECCCHHHHCCCCCEEE
VAGTERAVLMVESEAQQLSEEIMLGAVVYGHEQMQIAINAIHDLVRDGGKPEWDWQAAPK
EECCCEEEEEECHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
NEALVAKVSELGLADLQAAYQLRQKSARSQKLKEVYKSVAAKLAEAGVEADGVEVDNILF
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
ELESKIVRGQILNGEPRIDGRDTRTVRPIEIRSSVLPRAHGSSLFTRGETQALVVAALGT
HHHHHHHHCEEECCCCCCCCCCCCEECCCHHHHHHCCCCCCCCCEECCCCCEEEEEEECC
KSDEQIIDALQGEYRDRFMLHYNMPPFATGETGRVGSPKRREVGHGRLAKRALIPVLPPA
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCC
DEFGYTIRLVSEITESNGSSSMASVCGGSLALMDAGVPIKAHVAGVAMGLILEDNKFAVL
CCCCHHHHHHHHHHHCCCCCHHHHHCCCCEEEEECCCCHHHHHHHEEEEEEEECCCEEEE
TDILGDEDHLGDMDFKVAGTDAGITALQMDIKVQGITKEIMQVALAQAKEGRLHILGKMQ
EECCCCCCCCCCCCEEEECCCCCCEEEEEEEEEEHHHHHHHHHHHHHCCCCCEEEEEHHH
AAMGGARTELSEHAPRMITVKINPEKIRDVIGKGGSTIQALTKETGCTIDIGEDGTITIA
HHHCCHHHHHHHCCCEEEEEEECHHHHHHHHCCCCCHHHHHHHCCCCEEEECCCCEEEEE
STSSEGMAEAKRRIEGITAEAEVGKIYNGTVLKLLDFGAIVNILPGKDGLLHISEIANER
ECCCCHHHHHHHHHHCCCCCHHCCCCCCCHHHHHHHHCHHEEECCCCCCCEEHHHHHHHH
VNQVSDYVKEGQAVRVKLLSTDEKGRMRLSIKAAKAEEGDVPVAAPQAPGAGDAASQQQQ
HHHHHHHHHCCCEEEEEEEECCCCCCEEEEEEECCCCCCCCCEECCCCCCCCCHHHHHHH
QQQQ
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA