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The map label for this gene is nudF [H]

Identifier: 187929278

GI number: 187929278

Start: 2308836

End: 2309447

Strand: Reverse

Name: nudF [H]

Synonym: Rpic_2199

Alternate gene names: 187929278

Gene position: 2309447-2308836 (Counterclockwise)

Preceding gene: 187929279

Following gene: 187929277

Centisome position: 58.58

GC content: 60.95

Gene sequence:

>612_bases
ATGTCCGATCACACCCCTCTGAACGAAGCCCCTGATGCGACGCAGGACCCCGACGCCGGTCTGCGCGAGACGCAAGTTGC
ATCCGCGCTGATGCACCAGGGTAAATTTCTCACGCTCAAGCACGACATCGTCCGCCTGCCGGACGGCCGCAACGCCAGCC
GCGAATATCTCATCCACCCAGGCGCGGTAATGATGATCCCGTTGTTCGACGACGGTACGGTGCTGATGGAGCGTCAGTTC
CGCTACCCGGTTGGCAAGGTGATGATTGAATTCCCCGCCGGCAAGCTGGATCCGCAGGAAGGCGCGCTCACATGCGGCAA
GCGCGAACTGCGCGAGGAAACCGGCTACACGGCCGAGCGCTGGGAGTACCTCACGCGCATTCACCCCGTCATTTCGTATT
CCACCGAGTTCATCGACATCTATCTCGCGCGCGATCTCAAGCAGGGCGCGGCAATGCTGGATGAAGGCGAATTTCTCGAG
ACCTTCGTCACGCCGGCCGGCCAACTGATCGATTGGGTGCGCGAAGGCAAGATTTCCGACGTGAAGACCATCATCGGCAC
GTTCTGGCTCGAGAAGATTCTGTCCGGAGCATGGACGCCAGGTGCCGTCTGA

Upstream 100 bases:

>100_bases
CGTTTCCGGGCTTTACGTGGCAATACCTGGTTCGCCATCCCCGCGATTCCCGTTAATTCCCGCCGGGCAGGTTCCCGCTG
TCTCGGCATGAGAGAGCGCC

Downstream 100 bases:

>100_bases
GCCGCTTGTGATCCTCGCTTGATCAGACCGTTTTCCTATTGCCGCTATCATGGCGAACGGTCGTGCGAAAAAATTAGCAC
GACCGTTCACAAATTTTCGA

Product: NUDIX hydrolase

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 203; Mature: 202

Protein sequence:

>203_residues
MSDHTPLNEAPDATQDPDAGLRETQVASALMHQGKFLTLKHDIVRLPDGRNASREYLIHPGAVMMIPLFDDGTVLMERQF
RYPVGKVMIEFPAGKLDPQEGALTCGKRELREETGYTAERWEYLTRIHPVISYSTEFIDIYLARDLKQGAAMLDEGEFLE
TFVTPAGQLIDWVREGKISDVKTIIGTFWLEKILSGAWTPGAV

Sequences:

>Translated_203_residues
MSDHTPLNEAPDATQDPDAGLRETQVASALMHQGKFLTLKHDIVRLPDGRNASREYLIHPGAVMMIPLFDDGTVLMERQF
RYPVGKVMIEFPAGKLDPQEGALTCGKRELREETGYTAERWEYLTRIHPVISYSTEFIDIYLARDLKQGAAMLDEGEFLE
TFVTPAGQLIDWVREGKISDVKTIIGTFWLEKILSGAWTPGAV
>Mature_202_residues
SDHTPLNEAPDATQDPDAGLRETQVASALMHQGKFLTLKHDIVRLPDGRNASREYLIHPGAVMMIPLFDDGTVLMERQFR
YPVGKVMIEFPAGKLDPQEGALTCGKRELREETGYTAERWEYLTRIHPVISYSTEFIDIYLARDLKQGAAMLDEGEFLET
FVTPAGQLIDWVREGKISDVKTIIGTFWLEKILSGAWTPGAV

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789800, Length=137, Percent_Identity=28.4671532846715, Blast_Score=60, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 22809; Mature: 22678

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDHTPLNEAPDATQDPDAGLRETQVASALMHQGKFLTLKHDIVRLPDGRNASREYLIHP
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEECC
GAVMMIPLFDDGTVLMERQFRYPVGKVMIEFPAGKLDPQEGALTCGKRELREETGYTAER
CEEEEEEEECCCEEEEHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
WEYLTRIHPVISYSTEFIDIYLARDLKQGAAMLDEGEFLETFVTPAGQLIDWVREGKISD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCHH
VKTIIGTFWLEKILSGAWTPGAV
HHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDHTPLNEAPDATQDPDAGLRETQVASALMHQGKFLTLKHDIVRLPDGRNASREYLIHP
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCEEECCCCCCCCCEEEECC
GAVMMIPLFDDGTVLMERQFRYPVGKVMIEFPAGKLDPQEGALTCGKRELREETGYTAER
CEEEEEEEECCCEEEEHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCHHH
WEYLTRIHPVISYSTEFIDIYLARDLKQGAAMLDEGEFLETFVTPAGQLIDWVREGKISD
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCCHHHHHHHHHCCCCHH
VKTIIGTFWLEKILSGAWTPGAV
HHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377; 10542272 [H]