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The map label for this gene is ureF

Identifier: 187929261

GI number: 187929261

Start: 2293191

End: 2293880

Strand: Reverse

Name: ureF

Synonym: Rpic_2182

Alternate gene names: 187929261

Gene position: 2293880-2293191 (Counterclockwise)

Preceding gene: 187929262

Following gene: 187929260

Centisome position: 58.18

GC content: 68.12

Gene sequence:

>690_bases
ATGACTAACGCGGCGCAGCTCACCGCCTTGCTGCATCTCGCGTCCCCGGCGCTGCCGGTGGGCGCGTTCAGCTATTCGCA
GGGGCTGGAGGCGGCGGTGGATGTGCGGCGCGTGGCGGACGAAGCGTCGGCTGCCGCGTGGATTGCCGAGGGGCTCGACG
TGCTCGCCGCATGCGAGGCACCGCTATGGCTGCTGCAGTTTGCCGACTGGCAGGCAGGCCGCTTCGATGCCGTTGCCGAG
CGCGATGCGTGGTTCCTCGCCACCCGCGAGACGCGCGAGCTGCGGCTCGAAACGTCTCAGATGGGCTGGTCGCTCAATCG
CCTCATCCAGCAGATGGCGTGGGGCGATGACGCACTGCGCAGTGCCTTGGCCGCATGCGCTTCGGTGACGTTTCCGACCG
CGTTTGCCGCCGCTGCTGCCGCGCTGAATGTCGACCCGCGCGATGGCGTGACGGCGTACTGCTTTGCCTGGGTGGAAAAC
CAGATGGCGGCGGCAGTCAAGGCCGTGCCGCTCGGGCAGGCTGCGGGGCAACGGATTCTCTTTGGCCTGCACGCAGCGGT
GGCGCGTGCGGTGGAGGAGGCGACACGCCGCGCGGCCTGTCATCCGCCTGAACTGTCTACGTTTTCGCCGGGGCTGGGCG
TGCTGTCCGCCCGGCATGAAACGCAATACTCACGACTCTTTCGTTCCTGA

Upstream 100 bases:

>100_bases
CACGATCATGGTCACTCGCATGGCCACGATCACGCACATGGTCACGACCACGACGACGGCCATGTCCATGGGCCCGGTTG
CGGCCATCACCACCATCACC

Downstream 100 bases:

>100_bases
ACCTGATCATCATGCGAACCAAGAAACTTCCTGCCCTGCGCGTTGGCGTGGGCGGCCCGGTGGGCTCCGGCAAGACCACG
CTGCTCGAAATGCTATGCAA

Product: Urease accessory protein UreF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 229; Mature: 228

Protein sequence:

>229_residues
MTNAAQLTALLHLASPALPVGAFSYSQGLEAAVDVRRVADEASAAAWIAEGLDVLAACEAPLWLLQFADWQAGRFDAVAE
RDAWFLATRETRELRLETSQMGWSLNRLIQQMAWGDDALRSALAACASVTFPTAFAAAAAALNVDPRDGVTAYCFAWVEN
QMAAAVKAVPLGQAAGQRILFGLHAAVARAVEEATRRAACHPPELSTFSPGLGVLSARHETQYSRLFRS

Sequences:

>Translated_229_residues
MTNAAQLTALLHLASPALPVGAFSYSQGLEAAVDVRRVADEASAAAWIAEGLDVLAACEAPLWLLQFADWQAGRFDAVAE
RDAWFLATRETRELRLETSQMGWSLNRLIQQMAWGDDALRSALAACASVTFPTAFAAAAAALNVDPRDGVTAYCFAWVEN
QMAAAVKAVPLGQAAGQRILFGLHAAVARAVEEATRRAACHPPELSTFSPGLGVLSARHETQYSRLFRS
>Mature_228_residues
TNAAQLTALLHLASPALPVGAFSYSQGLEAAVDVRRVADEASAAAWIAEGLDVLAACEAPLWLLQFADWQAGRFDAVAER
DAWFLATRETRELRLETSQMGWSLNRLIQQMAWGDDALRSALAACASVTFPTAFAAAAAALNVDPRDGVTAYCFAWVENQ
MAAAVKAVPLGQAAGQRILFGLHAAVARAVEEATRRAACHPPELSTFSPGLGVLSARHETQYSRLFRS

Specific function: Required for maturation of urease via the functional incorporation of the urease nickel metallocenter

COG id: COG0830

COG function: function code O; Urease accessory protein UreF

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ureF family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UREF_RALPJ (B2U7M9)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899748.1
- GeneID:   6287333
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2182
- HOGENOM:   HBG709295
- OMA:   SPQLPIG
- ProtClustDB:   CLSK897184
- GO:   GO:0005737
- HAMAP:   MF_01385
- InterPro:   IPR002639
- PIRSF:   PIRSF009467

Pfam domain/function: PF01730 UreF

EC number: NA

Molecular weight: Translated: 24515; Mature: 24384

Theoretical pI: Translated: 5.28; Mature: 5.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNAAQLTALLHLASPALPVGAFSYSQGLEAAVDVRRVADEASAAAWIAEGLDVLAACEA
CCCHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLWLLQFADWQAGRFDAVAERDAWFLATRETRELRLETSQMGWSLNRLIQQMAWGDDALR
CHHHHHHCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHH
SALAACASVTFPTAFAAAAAALNVDPRDGVTAYCFAWVENQMAAAVKAVPLGQAAGQRIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FGLHAAVARAVEEATRRAACHPPELSTFSPGLGVLSARHETQYSRLFRS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TNAAQLTALLHLASPALPVGAFSYSQGLEAAVDVRRVADEASAAAWIAEGLDVLAACEA
CCHHHHHHHHHHHCCCCCCCCCHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PLWLLQFADWQAGRFDAVAERDAWFLATRETRELRLETSQMGWSLNRLIQQMAWGDDALR
CHHHHHHCCCCCCCCHHHHCCCEEEEEECCHHHHHHHHHHHCHHHHHHHHHHHCCCHHHH
SALAACASVTFPTAFAAAAAALNVDPRDGVTAYCFAWVENQMAAAVKAVPLGQAAGQRIL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
FGLHAAVARAVEEATRRAACHPPELSTFSPGLGVLSARHETQYSRLFRS
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA