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The map label for this gene is dmlR [H]

Identifier: 187929253

GI number: 187929253

Start: 2286542

End: 2287537

Strand: Direct

Name: dmlR [H]

Synonym: Rpic_2174

Alternate gene names: 187929253

Gene position: 2286542-2287537 (Clockwise)

Preceding gene: 187929250

Following gene: 187929255

Centisome position: 58.0

GC content: 69.98

Gene sequence:

>996_bases
ATGATCGCCCTCGACGACCTCCGACTGCTGGTGCGCATCGCCGACGCCGGCAACCTTTCCGCCGCTGCGCGCCAGCTCGG
TTGGCTGCCCGCCACCGCCAGCGCAGCGCTCAAGCGCGTGGAGACCGAACTCGGCGCCCGCCTGTTCGAGCGCACCACGC
GCAGCATGCGGCCGACCGAAGCCGGCCAGCGTTATCTAGGGTATGCAAGACAGGCGCTGGAGGCACTGGACGAAGGCGCC
GAAAGCCTGGGGCAGGACCGCACCGAGCTGTCCGGCCCCATCCGCATTGCCGCCACATCGGACCTCGGGCGACACATCCT
GCGCCCATGGCTCGACGCCTTTTGCGATGAGCACCCCGGCGTGCGGATCACGCTGGTGCTGGGCGACCGGCTGGCCGACC
TGATGCGCGAAGACCTCGACTTTGCGCTGCGCTACGGGCATCTGCAGGATTCGGCGCTGGTGCGGCGGCCCCTTGCGTCA
CATCCGCGCGTGATCGTGGGCGCGCCGTCGTACTTCAAGAAGCATGGCCGGCCTGCACACCCGAGCGACCTCGCCGACCA
CCGCTGCCTGATCCTCCTGCGCAACGGCGAGCCCGTCACGCGCTGGCAACTCACGCACGCCAGCGGCCCTGCCCCGATCG
ACGTACGCGGCGATTTCCAATGCGACGACGGCGCCGTGGTGCGCGACTGGGCGGTCGCCGGGCGCGGCCTGGCATTCAAA
TCGTGGCTGGATGTCGCGCAAGACGTGAGCGCAGGCCGGCTGGAGGTGGCATTGCCGGCGTGGAGCCATGCGCCCACGCC
GCTGCAGCTCGTGGCCCTTGCGCGCCGCCATCGGCCTGCGCGGCTGACGGCGTGTGCGGACTTTCTCGCCGCGCGCTTTG
CCGAGTTCAGCGCGCGTTATCCGTTCCCGTCCGCACAGGGGGCAGCGGCATCGCAAAAGCTGGCCAAGGCGGCCAAGCCG
ACCAAAACCAAGTCGGCCGTCACCGCGAACACGTAG

Upstream 100 bases:

>100_bases
GAGGAAAGAAAAAACTGTCAATCAATTGGGCCATTCTTTCATCAAACAAAAGCAAGATAATCTGGCTATGCTTTGATGAT
TCTCCAAATCTGCTTTGACA

Downstream 100 bases:

>100_bases
GTGTCCTTAATGGTTGTTGCGGCGGCGGACCACCCACCACGCAACGACCACGGTGAATGCCGCCACCAGCCCCACCATCA
GCCAGAAACCGGGGCACACC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: D-malate degradation protein R [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MIALDDLRLLVRIADAGNLSAAARQLGWLPATASAALKRVETELGARLFERTTRSMRPTEAGQRYLGYARQALEALDEGA
ESLGQDRTELSGPIRIAATSDLGRHILRPWLDAFCDEHPGVRITLVLGDRLADLMREDLDFALRYGHLQDSALVRRPLAS
HPRVIVGAPSYFKKHGRPAHPSDLADHRCLILLRNGEPVTRWQLTHASGPAPIDVRGDFQCDDGAVVRDWAVAGRGLAFK
SWLDVAQDVSAGRLEVALPAWSHAPTPLQLVALARRHRPARLTACADFLAARFAEFSARYPFPSAQGAAASQKLAKAAKP
TKTKSAVTANT

Sequences:

>Translated_331_residues
MIALDDLRLLVRIADAGNLSAAARQLGWLPATASAALKRVETELGARLFERTTRSMRPTEAGQRYLGYARQALEALDEGA
ESLGQDRTELSGPIRIAATSDLGRHILRPWLDAFCDEHPGVRITLVLGDRLADLMREDLDFALRYGHLQDSALVRRPLAS
HPRVIVGAPSYFKKHGRPAHPSDLADHRCLILLRNGEPVTRWQLTHASGPAPIDVRGDFQCDDGAVVRDWAVAGRGLAFK
SWLDVAQDVSAGRLEVALPAWSHAPTPLQLVALARRHRPARLTACADFLAARFAEFSARYPFPSAQGAAASQKLAKAAKP
TKTKSAVTANT
>Mature_331_residues
MIALDDLRLLVRIADAGNLSAAARQLGWLPATASAALKRVETELGARLFERTTRSMRPTEAGQRYLGYARQALEALDEGA
ESLGQDRTELSGPIRIAATSDLGRHILRPWLDAFCDEHPGVRITLVLGDRLADLMREDLDFALRYGHLQDSALVRRPLAS
HPRVIVGAPSYFKKHGRPAHPSDLADHRCLILLRNGEPVTRWQLTHASGPAPIDVRGDFQCDDGAVVRDWAVAGRGLAFK
SWLDVAQDVSAGRLEVALPAWSHAPTPLQLVALARRHRPARLTACADFLAARFAEFSARYPFPSAQGAAASQKLAKAAKP
TKTKSAVTANT

Specific function: Transcriptional regulator required for the aerobic growth on D-malate as the sole carbon source. Induces the expression of dmlA in response to D-malate or L- or meso-tartrate. Negatively regulates its own expression [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87081978, Length=302, Percent_Identity=30.4635761589404, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI1789639, Length=293, Percent_Identity=29.0102389078498, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1786401, Length=290, Percent_Identity=29.6551724137931, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1789440, Length=288, Percent_Identity=27.7777777777778, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1787128, Length=290, Percent_Identity=26.2068965517241, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI1787589, Length=291, Percent_Identity=28.8659793814433, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI145693193, Length=297, Percent_Identity=26.5993265993266, Blast_Score=111, Evalue=8e-26,
Organism=Escherichia coli, GI1789173, Length=252, Percent_Identity=27.7777777777778, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786448, Length=252, Percent_Identity=24.2063492063492, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1790011, Length=299, Percent_Identity=23.7458193979933, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787601, Length=145, Percent_Identity=25.5172413793103, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1788706, Length=159, Percent_Identity=25.7861635220126, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790208, Length=126, Percent_Identity=33.3333333333333, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI157672245, Length=204, Percent_Identity=25.4901960784314, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36047; Mature: 36047

Theoretical pI: Translated: 9.93; Mature: 9.93

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIALDDLRLLVRIADAGNLSAAARQLGWLPATASAALKRVETELGARLFERTTRSMRPTE
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AGQRYLGYARQALEALDEGAESLGQDRTELSGPIRIAATSDLGRHILRPWLDAFCDEHPG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCC
VRITLVLGDRLADLMREDLDFALRYGHLQDSALVRRPLASHPRVIVGAPSYFKKHGRPAH
EEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCEEEEECCHHHHHCCCCCC
PSDLADHRCLILLRNGEPVTRWQLTHASGPAPIDVRGDFQCDDGAVVRDWAVAGRGLAFK
CCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCEEHHHHHHCCCCHHH
SWLDVAQDVSAGRLEVALPAWSHAPTPLQLVALARRHRPARLTACADFLAARFAEFSARY
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
PFPSAQGAAASQKLAKAAKPTKTKSAVTANT
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MIALDDLRLLVRIADAGNLSAAARQLGWLPATASAALKRVETELGARLFERTTRSMRPTE
CCCHHHHHHHHHCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
AGQRYLGYARQALEALDEGAESLGQDRTELSGPIRIAATSDLGRHILRPWLDAFCDEHPG
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCC
VRITLVLGDRLADLMREDLDFALRYGHLQDSALVRRPLASHPRVIVGAPSYFKKHGRPAH
EEEEEEECHHHHHHHHHHHHHHHHHCCCCHHHHHHCHHHCCCEEEEECCHHHHHCCCCCC
PSDLADHRCLILLRNGEPVTRWQLTHASGPAPIDVRGDFQCDDGAVVRDWAVAGRGLAFK
CCCCCCCEEEEEEECCCCCEEEEEECCCCCCCEECCCCCCCCCCCEEHHHHHHCCCCHHH
SWLDVAQDVSAGRLEVALPAWSHAPTPLQLVALARRHRPARLTACADFLAARFAEFSARY
HHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCC
PFPSAQGAAASQKLAKAAKPTKTKSAVTANT
CCCCCCCCHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503 [H]