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The map label for this gene is prpB [H]

Identifier: 187929234

GI number: 187929234

Start: 2267107

End: 2268003

Strand: Direct

Name: prpB [H]

Synonym: Rpic_2155

Alternate gene names: 187929234

Gene position: 2267107-2268003 (Clockwise)

Preceding gene: 187929233

Following gene: 187929235

Centisome position: 57.5

GC content: 62.54

Gene sequence:

>897_bases
ATGATGGCAAATACCCATGCCAGTGCAGGCGCCCGCTTCCGCCAGGCGCTGGTCGATGAGTCGCCGCTGCAGGTGGTCGG
CACCATCAACGCCAACCACGCGCTGCTGGCCAAGCGCGCCGGCTATCGCGCGATCTACCTGTCGGGCGGCGGCGTGGCAG
CTGGTTCGCTGGGCTTGCCGGACCTCGGCATATCGGGCCTGGACGACGTTTTGACCGACGTCCGCCGCATCACCGATGTA
TGCGACGTACCGCTGCTGGTAGATGTCGACACGGGCTTCGGCGCTTCCGCCTTCAACGTGGCACGCACGACCAAGTCACT
CATCAAGTTTGGCGCAGCGGCCATGCACATTGAAGACCAGGTCGGCGCCAAGCGCTGCGGCCATCGCCCCAACAAGGAGA
TCGTTTCTAAGGACGAAATGGTCGATCGCATCAAGGCTGCCTTAGATGCCCGCACCGACGCGAGCTTTGTCGTCATGGCC
CGCACCGATGCGCTGGCCGTCGAAGGGTTCGACCGCGCCCTGGAACGCGCCGTGGCCTGCGTGGAAGCTGGTGCGGACGC
GATCTTTCCCGAGGCGATGACGGAACTGTCGATGTACCGGAAGTTTGTCGACGCGGTAAAGGTGCCGGTGCTAGCCAACA
TCACCGAATTTGGGCAGACGCCGCTTTTCACGCGCGATGAGCTGGCATCCGCAGGTGTGTCGATGATCCTGTATCCGCTA
TCGGCATTCCGCGCGATGAACAAGGCAGCGGAGAACGTCTACACCGCCATTCGTCGCGATGGCACGCAACAGAACGTCGT
CGACACCATGCAGACGCGCATGGAACTCTACGACAGCATCGGTTACCACGACTACGAAGCCAAGCTGGACGCGCTGTTCG
CACAGAACCGCGGATGA

Upstream 100 bases:

>100_bases
GGTGGGCCGTGCTGCAGCGCGCGCACACGACCGGTGTGGAGTTGCCGGCCAACGCCGTCGCGCGATTCTTCGCGTGACAG
CCACAGAGAGGAGATTCGCC

Downstream 100 bases:

>100_bases
ACCGGCCAGCATCCATACCAACACAAACGAGGAGAACGGAAGGCAAAACCGCGGAGTAAGCTGCCTAGGAACGACCCAAA
AAACGATGTTGATGCTTGCA

Product: 2-methylisocitrate lyase

Products: NA

Alternate protein names: 2-methylisocitrate lyase [H]

Number of amino acids: Translated: 298; Mature: 298

Protein sequence:

>298_residues
MMANTHASAGARFRQALVDESPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLDDVLTDVRRITDV
CDVPLLVDVDTGFGASAFNVARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAALDARTDASFVVMA
RTDALAVEGFDRALERAVACVEAGADAIFPEAMTELSMYRKFVDAVKVPVLANITEFGQTPLFTRDELASAGVSMILYPL
SAFRAMNKAAENVYTAIRRDGTQQNVVDTMQTRMELYDSIGYHDYEAKLDALFAQNRG

Sequences:

>Translated_298_residues
MMANTHASAGARFRQALVDESPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLDDVLTDVRRITDV
CDVPLLVDVDTGFGASAFNVARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAALDARTDASFVVMA
RTDALAVEGFDRALERAVACVEAGADAIFPEAMTELSMYRKFVDAVKVPVLANITEFGQTPLFTRDELASAGVSMILYPL
SAFRAMNKAAENVYTAIRRDGTQQNVVDTMQTRMELYDSIGYHDYEAKLDALFAQNRG
>Mature_298_residues
MMANTHASAGARFRQALVDESPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLPDLGISGLDDVLTDVRRITDV
CDVPLLVDVDTGFGASAFNVARTTKSLIKFGAAAMHIEDQVGAKRCGHRPNKEIVSKDEMVDRIKAALDARTDASFVVMA
RTDALAVEGFDRALERAVACVEAGADAIFPEAMTELSMYRKFVDAVKVPVLANITEFGQTPLFTRDELASAGVSMILYPL
SAFRAMNKAAENVYTAIRRDGTQQNVVDTMQTRMELYDSIGYHDYEAKLDALFAQNRG

Specific function: Catalyzes the formation of pyruvate and succinate from 2-methylisocitrate [H]

COG id: COG2513

COG function: function code G; PEP phosphonomutase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family [H]

Homologues:

Organism=Escherichia coli, GI1786525, Length=290, Percent_Identity=75.8620689655172, Blast_Score=462, Evalue=1e-131,
Organism=Escherichia coli, GI1790445, Length=264, Percent_Identity=26.1363636363636, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000918
- InterPro:   IPR018523
- InterPro:   IPR012695
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00463 ICL [H]

EC number: =4.1.3.30 [H]

Molecular weight: Translated: 32099; Mature: 32099

Theoretical pI: Translated: 5.41; Mature: 5.41

Prosite motif: PS00161 ISOCITRATE_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMANTHASAGARFRQALVDESPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLP
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCEEEEEECCCEEECCCCCC
DLGISGLDDVLTDVRRITDVCDVPLLVDVDTGFGASAFNVARTTKSLIKFGAAAMHIEDQ
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGAKRCGHRPNKEIVSKDEMVDRIKAALDARTDASFVVMARTDALAVEGFDRALERAVAC
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VEAGADAIFPEAMTELSMYRKFVDAVKVPVLANITEFGQTPLFTRDELASAGVSMILYPL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHH
SAFRAMNKAAENVYTAIRRDGTQQNVVDTMQTRMELYDSIGYHDYEAKLDALFAQNRG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure
MMANTHASAGARFRQALVDESPLQVVGTINANHALLAKRAGYRAIYLSGGGVAAGSLGLP
CCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHCCCCEEEEEECCCEEECCCCCC
DLGISGLDDVLTDVRRITDVCDVPLLVDVDTGFGASAFNVARTTKSLIKFGAAAMHIEDQ
CCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
VGAKRCGHRPNKEIVSKDEMVDRIKAALDARTDASFVVMARTDALAVEGFDRALERAVAC
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHHHHH
VEAGADAIFPEAMTELSMYRKFVDAVKVPVLANITEFGQTPLFTRDELASAGVSMILYPL
HHHCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHH
SAFRAMNKAAENVYTAIRRDGTQQNVVDTMQTRMELYDSIGYHDYEAKLDALFAQNRG
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9278503; 9325432 [H]