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The map label for this gene is truA

Identifier: 187929218

GI number: 187929218

Start: 2249437

End: 2250261

Strand: Reverse

Name: truA

Synonym: Rpic_2139

Alternate gene names: 187929218

Gene position: 2250261-2249437 (Counterclockwise)

Preceding gene: 187929219

Following gene: 187929217

Centisome position: 57.08

GC content: 67.15

Gene sequence:

>825_bases
ATGACGCGCATCGCGCTCGGCATCCAGTACGACGGTGCCGCGTTTTCAGGGTGGCAATCGCAGCCGCACGGCAATACCGT
TCAGGACGCGCTGGAGTCGGCGCTGCGGCAGTTTGCCGGCGTGGCGCTGCCGACCACGGTGGCCGGGCGAACCGATGCTG
GTGTGCATGCGCTGGGCCAGGTGGTCCACCTGGATACCGACCTCGTGCGCGAGCCGTTTTCGTGGGTGCGCGGCACCAAT
GCCTTTCTGCCGCCGACGGTGGCCGTGCGATGGGCGCAGGAGATGCCGGAGGATTTTCATGCGCGTTTCTCCGCGCATCG
CCGCACGTATTACTACGCGCTGACCTTCGGGCCGACGCGCGCGCCGCTGCTGGAGGACAAGGCCGGCTACGTGATGCTGC
CGCCCGGCCAATCCCTTGACGTGGCAGCCATGAACGAGGCGGCCCAGGTTCTGGTAGGCGAGCACGATTTCTCGTCGTTC
CGCGCGGCCGAATGCCAAGCCAAGTCGCCCGTCAAAACGATGTATTCGATCGAGGTGCGAGGCGAGGGCGAATGGGTGTT
CGTGCGCATTCGCGGCAGCGCGTTCCTGCACCACATGGTGCGCAACATCATGGGCTGCCTCGTGGCGATCGGGCGCGGCC
GCCAGCCGGTGTCCTGGATGGGCGATGTGCTGGCCGCCCGCAGCCGCGTGGCCGCCGCGCCAACTTTCATGCCCGACGGC
CTGTATCTCGCCGAGGTCGGCTATCCTGATGCTTTCTCGTTGCCGGCGTCTCCGGCATCGTCCAGCCTGTTTCGCGGCGT
GTTCGACGAGCACGCCGGCCTGTGA

Upstream 100 bases:

>100_bases
TGCGCCAGATCGGCTGACGCACTGGGCTCTACGCCATCGACAGCGCCGGCAGGGTGGTATCCTGCCGGCGTTTTTCTTTC
CGCATGGGGACTCACAAGGC

Downstream 100 bases:

>100_bases
ATCCCGCTTCCGCCATGCCGTTGCACCGTACCCGTATCAAGCTGTGTGGCCTGACCCAGCCTGCCGATGTCGATCACGCC
GTCGCAATCGGCGCCGATGC

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 274; Mature: 273

Protein sequence:

>274_residues
MTRIALGIQYDGAAFSGWQSQPHGNTVQDALESALRQFAGVALPTTVAGRTDAGVHALGQVVHLDTDLVREPFSWVRGTN
AFLPPTVAVRWAQEMPEDFHARFSAHRRTYYYALTFGPTRAPLLEDKAGYVMLPPGQSLDVAAMNEAAQVLVGEHDFSSF
RAAECQAKSPVKTMYSIEVRGEGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGRQPVSWMGDVLAARSRVAAAPTFMPDG
LYLAEVGYPDAFSLPASPASSSLFRGVFDEHAGL

Sequences:

>Translated_274_residues
MTRIALGIQYDGAAFSGWQSQPHGNTVQDALESALRQFAGVALPTTVAGRTDAGVHALGQVVHLDTDLVREPFSWVRGTN
AFLPPTVAVRWAQEMPEDFHARFSAHRRTYYYALTFGPTRAPLLEDKAGYVMLPPGQSLDVAAMNEAAQVLVGEHDFSSF
RAAECQAKSPVKTMYSIEVRGEGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGRQPVSWMGDVLAARSRVAAAPTFMPDG
LYLAEVGYPDAFSLPASPASSSLFRGVFDEHAGL
>Mature_273_residues
TRIALGIQYDGAAFSGWQSQPHGNTVQDALESALRQFAGVALPTTVAGRTDAGVHALGQVVHLDTDLVREPFSWVRGTNA
FLPPTVAVRWAQEMPEDFHARFSAHRRTYYYALTFGPTRAPLLEDKAGYVMLPPGQSLDVAAMNEAAQVLVGEHDFSSFR
AAECQAKSPVKTMYSIEVRGEGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGRQPVSWMGDVLAARSRVAAAPTFMPDGL
YLAEVGYPDAFSLPASPASSSLFRGVFDEHAGL

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=279, Percent_Identity=32.6164874551971, Blast_Score=102, Evalue=4e-22,
Organism=Homo sapiens, GI199559785, Length=264, Percent_Identity=29.9242424242424, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788657, Length=256, Percent_Identity=46.484375, Blast_Score=238, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI71985046, Length=268, Percent_Identity=28.3582089552239, Blast_Score=78, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_RALPJ (B2U6Z1)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899705.1
- ProteinModelPortal:   B2U6Z1
- SMR:   B2U6Z1
- GeneID:   6287815
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2139
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 29811; Mature: 29680

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: NA

Important sites: ACT_SITE 52-52

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRIALGIQYDGAAFSGWQSQPHGNTVQDALESALRQFAGVALPTTVAGRTDAGVHALGQ
CCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHH
VVHLDTDLVREPFSWVRGTNAFLPPTVAVRWAQEMPEDFHARFSAHRRTYYYALTFGPTR
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEECCCC
APLLEDKAGYVMLPPGQSLDVAAMNEAAQVLVGEHDFSSFRAAECQAKSPVKTMYSIEVR
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHHCCCCCCCEEEEEEEEE
GEGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGRQPVSWMGDVLAARSRVAAAPTFMPDG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LYLAEVGYPDAFSLPASPASSSLFRGVFDEHAGL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TRIALGIQYDGAAFSGWQSQPHGNTVQDALESALRQFAGVALPTTVAGRTDAGVHALGQ
CEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCEECCCCCCCHHHHHH
VVHLDTDLVREPFSWVRGTNAFLPPTVAVRWAQEMPEDFHARFSAHRRTYYYALTFGPTR
HHCCCHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEEEEEEEECCCC
APLLEDKAGYVMLPPGQSLDVAAMNEAAQVLVGEHDFSSFRAAECQAKSPVKTMYSIEVR
CCCCCCCCCEEEECCCCCCCHHHHHHHHHHEECCCCCCHHHHHHCCCCCCCEEEEEEEEE
GEGEWVFVRIRGSAFLHHMVRNIMGCLVAIGRGRQPVSWMGDVLAARSRVAAAPTFMPDG
CCCCEEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LYLAEVGYPDAFSLPASPASSSLFRGVFDEHAGL
EEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA