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The map label for this gene is trpA

Identifier: 187929214

GI number: 187929214

Start: 2245781

End: 2246578

Strand: Reverse

Name: trpA

Synonym: Rpic_2135

Alternate gene names: 187929214

Gene position: 2246578-2245781 (Counterclockwise)

Preceding gene: 187929215

Following gene: 187929213

Centisome position: 56.98

GC content: 63.41

Gene sequence:

>798_bases
ATGTCCCGCATCGCTCAAACCTTCTCACAACTGTCTGCGCAGGGCCGCAAGGGCCTGATTCCGTTCATCACGGCCGGCGA
TCCGTACCCCGAGATGACGGTCGACCTGATGCACGCGCTGGTCAAGGGCGGCTCCAACGTCATCGAACTCGGCGTGCCGT
TCTCGGACCCGATGGCCGATGGCCCGGTGATCCAACGTGCGTCCGAGCGTGCATTGGCCAAGAAGGTGGGCCTGCGCACG
GTGCTCGAATATGTGCGCGCATTCCGCGCCACCGATTTGACGACGCCCGTGGTGCTCATGGGTTACGCCAATCCGATCGA
GCGCATGGGCGTTGATGCCTTTGCCAAGGCTGCATCCGAAGCGGGCGTGGACGGCGTGCTCGTGGTCGACTACCCGCCCG
AAGAATGCGAGGCCTTTGCCAAGACCATGCGCGCCGCCGGTATCGATCCGATCTTCCTGCTGGCGCCCACGTCCACCGAA
GCACGCATCGCGCAGATCGCCCGTGTGGCGAGCGGCTACATCTACTACGTGTCGCTCAAAGGCGTGACTGGCGCGGCCAC
CATCGACCTGGATGCGGTGGCTGCACGGATCCCGCAAATCCGCCAGCACGCGCGGCTGCCGGTAGGTGTCGGTTTCGGTA
TCCGTGATGCGGCAACGGCACGTGCGATCAGCGGTGTGGCGGATGCGGTGGTGATCGGATCCCGCATTGTGCAATTGCTT
GAAGAAGCACCTCGCGAACAAGCGGTACAATACTTGACTGATTTCATCGCGGAGATTCGCCAGGCGCTCGACGCCTGA

Upstream 100 bases:

>100_bases
AACGCGTGGCCGCTGTGCATGCCGCCGCTGCCACCAACGCCCAACCGATTGAATCGCAGGCGCCTGAGACCATCAAGGTC
GCCTGAAACCTGATCCTCCT

Downstream 100 bases:

>100_bases
TCTCGGCGAAACTGCATTCAAGGAGCACGCATGAGCTGGCTGGACAAACTGCTGCCGCCCAAGATCCAACAGACCGACCC
GTCGCAGCGCAAGGGCATTC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 265; Mature: 264

Protein sequence:

>265_residues
MSRIAQTFSQLSAQGRKGLIPFITAGDPYPEMTVDLMHALVKGGSNVIELGVPFSDPMADGPVIQRASERALAKKVGLRT
VLEYVRAFRATDLTTPVVLMGYANPIERMGVDAFAKAASEAGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTE
ARIAQIARVASGYIYYVSLKGVTGAATIDLDAVAARIPQIRQHARLPVGVGFGIRDAATARAISGVADAVVIGSRIVQLL
EEAPREQAVQYLTDFIAEIRQALDA

Sequences:

>Translated_265_residues
MSRIAQTFSQLSAQGRKGLIPFITAGDPYPEMTVDLMHALVKGGSNVIELGVPFSDPMADGPVIQRASERALAKKVGLRT
VLEYVRAFRATDLTTPVVLMGYANPIERMGVDAFAKAASEAGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTE
ARIAQIARVASGYIYYVSLKGVTGAATIDLDAVAARIPQIRQHARLPVGVGFGIRDAATARAISGVADAVVIGSRIVQLL
EEAPREQAVQYLTDFIAEIRQALDA
>Mature_264_residues
SRIAQTFSQLSAQGRKGLIPFITAGDPYPEMTVDLMHALVKGGSNVIELGVPFSDPMADGPVIQRASERALAKKVGLRTV
LEYVRAFRATDLTTPVVLMGYANPIERMGVDAFAKAASEAGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTEA
RIAQIARVASGYIYYVSLKGVTGAATIDLDAVAARIPQIRQHARLPVGVGFGIRDAATARAISGVADAVVIGSRIVQLLE
EAPREQAVQYLTDFIAEIRQALDA

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family

Homologues:

Organism=Escherichia coli, GI1787514, Length=250, Percent_Identity=35.2, Blast_Score=142, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321412, Length=261, Percent_Identity=36.7816091954023, Blast_Score=175, Evalue=5e-45,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPA_RALPJ (B2U6Y7)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899701.1
- ProteinModelPortal:   B2U6Y7
- SMR:   B2U6Y7
- GeneID:   6287064
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2135
- HOGENOM:   HBG391690
- OMA:   NPIYIYG
- ProtClustDB:   PRK13111
- HAMAP:   MF_00131
- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00262

Pfam domain/function: PF00290 Trp_syntA; SSF51366 RibP_bind_barrel

EC number: =4.2.1.20

Molecular weight: Translated: 28185; Mature: 28054

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: ACT_SITE 49-49 ACT_SITE 60-60

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIAQTFSQLSAQGRKGLIPFITAGDPYPEMTVDLMHALVKGGSNVIELGVPFSDPMAD
CCHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALAKKVGLRTVLEYVRAFRATDLTTPVVLMGYANPIERMGVDAFAKAASE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCHHHHHHHHHH
AGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTEARIAQIARVASGYIYYVSLK
CCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEC
GVTGAATIDLDAVAARIPQIRQHARLPVGVGFGIRDAATARAISGVADAVVIGSRIVQLL
CCCCCEEECHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAPREQAVQYLTDFIAEIRQALDA
HHCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRIAQTFSQLSAQGRKGLIPFITAGDPYPEMTVDLMHALVKGGSNVIELGVPFSDPMAD
CHHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPVIQRASERALAKKVGLRTVLEYVRAFRATDLTTPVVLMGYANPIERMGVDAFAKAASE
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCHHHHHCHHHHHHHHHH
AGVDGVLVVDYPPEECEAFAKTMRAAGIDPIFLLAPTSTEARIAQIARVASGYIYYVSLK
CCCCCEEEECCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCEEEEEEEC
GVTGAATIDLDAVAARIPQIRQHARLPVGVGFGIRDAATARAISGVADAVVIGSRIVQLL
CCCCCEEECHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
EEAPREQAVQYLTDFIAEIRQALDA
HHCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA