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The map label for this gene is rlmE

Identifier: 187929109

GI number: 187929109

Start: 2137937

End: 2138602

Strand: Reverse

Name: rlmE

Synonym: Rpic_2030

Alternate gene names: 187929109

Gene position: 2138602-2137937 (Counterclockwise)

Preceding gene: 187929111

Following gene: 187929108

Centisome position: 54.24

GC content: 59.61

Gene sequence:

>666_bases
ATGGCAAAGAACAAGTTTAACCAGTCGTGGCTGCACGACCATATCAACGATCCGTACGTCAAGCTGGCGCAGCGCGAAGG
CTATCGCGCACGTGCCGCCTACAAGCTCAAAGAGATCGACGAGCAGGACAAGTTGATCAAGCCGGGCCAGGTCATCGTTG
ACCTGGGTGCCGCGCCGGGCAGTTGGAGCCAGTACGTGCGCAACAAGCTGGCGGCCTCGCCGCGCGCCAAGGATGGACGT
ATCGACGGCGCGATCGTCGCCATCGACATCCTGCCGATGGAAGCCATCGCCGACGTTACCTTCATCCAGGGTGACTTCCG
CGAGGACGAGGTATTCCGGCAACTGGAAGAAATCGTGCTGGAAGTCAGCGGTGGCGGCAAAGTGGACCTTGTTTTGTCCG
ATATGGCCCCCAATCTCTCAGGTGTGGCGTCGGCAGACGCCGCGCGCATGGAGCACATCGCCGAGCTGGCAGTGGAATTT
GCCATGGCGCACCTGAAACCTGAAGGCGCGCTGCTGATCAAATGTTTCCACGGCAGCGGCTATAGTCAGATCGTCGAGAT
GTTCAAGCGTCATTTCAAGATCGTGGCGCCGCGCAAGCCGAAAGCCTCCCGTGACAAATCGTCCGAGACATTCTTGTTGG
GCCGCCAGCTCAAGCATCCGGGCTGA

Upstream 100 bases:

>100_bases
GCGGGATCGCGCAAAGAGACAAACGGCGCGTATTATCCGCCAAAACCAGTCCTGGCCGCCATGTTTGGCGCTACATTCGG
GCGAAATTGCAACCAATTCG

Downstream 100 bases:

>100_bases
CGGACGATACTCAAAAAGGGGCCCCTCGGCTGGCACGGTGGTTCGTTGGTCTTGCGGGCAATCAACCCGCGGCGGTGTCC
GTGCATTACAATGCCAAGCA

Product: ribosomal RNA methyltransferase RrmJ/FtsJ

Products: NA

Alternate protein names: 23S rRNA Um2552 methyltransferase; rRNA (uridine-2'-O-)-methyltransferase

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGR
IDGAIVAIDILPMEAIADVTFIQGDFREDEVFRQLEEIVLEVSGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEF
AMAHLKPEGALLIKCFHGSGYSQIVEMFKRHFKIVAPRKPKASRDKSSETFLLGRQLKHPG

Sequences:

>Translated_221_residues
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGR
IDGAIVAIDILPMEAIADVTFIQGDFREDEVFRQLEEIVLEVSGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEF
AMAHLKPEGALLIKCFHGSGYSQIVEMFKRHFKIVAPRKPKASRDKSSETFLLGRQLKHPG
>Mature_220_residues
AKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPGSWSQYVRNKLAASPRAKDGRI
DGAIVAIDILPMEAIADVTFIQGDFREDEVFRQLEEIVLEVSGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFA
MAHLKPEGALLIKCFHGSGYSQIVEMFKRHFKIVAPRKPKASRDKSSETFLLGRQLKHPG

Specific function: Specifically methylates the uridine in position 2552 of 23S rRNA at the 2'-O position of the ribose in the fully assembled 50S ribosomal subunit

COG id: COG0293

COG function: function code J; 23S rRNA methylase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RlmE family

Homologues:

Organism=Homo sapiens, GI7019377, Length=207, Percent_Identity=33.8164251207729, Blast_Score=125, Evalue=3e-29,
Organism=Homo sapiens, GI29029591, Length=204, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI29029589, Length=204, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI7110661, Length=204, Percent_Identity=31.3725490196078, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI194097365, Length=197, Percent_Identity=29.4416243654822, Blast_Score=99, Evalue=2e-21,
Organism=Escherichia coli, GI1789569, Length=207, Percent_Identity=42.512077294686, Blast_Score=177, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI17554650, Length=204, Percent_Identity=34.3137254901961, Blast_Score=107, Evalue=5e-24,
Organism=Caenorhabditis elegans, GI17553474, Length=231, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71987550, Length=204, Percent_Identity=30.8823529411765, Blast_Score=98, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71987561, Length=204, Percent_Identity=30.8823529411765, Blast_Score=98, Evalue=4e-21,
Organism=Caenorhabditis elegans, GI17553860, Length=204, Percent_Identity=30.8823529411765, Blast_Score=98, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6319796, Length=205, Percent_Identity=31.219512195122, Blast_Score=103, Evalue=2e-23,
Organism=Saccharomyces cerevisiae, GI6319535, Length=199, Percent_Identity=31.6582914572864, Blast_Score=103, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321302, Length=283, Percent_Identity=25.0883392226148, Blast_Score=82, Evalue=7e-17,
Organism=Drosophila melanogaster, GI21356387, Length=217, Percent_Identity=33.6405529953917, Blast_Score=127, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24648639, Length=204, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24647580, Length=204, Percent_Identity=32.3529411764706, Blast_Score=102, Evalue=2e-22,
Organism=Drosophila melanogaster, GI18859957, Length=197, Percent_Identity=27.9187817258883, Blast_Score=86, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLME_RALPJ (B2UGQ0)

Other databases:

- EMBL:   CP001068
- RefSeq:   YP_001899596.1
- ProteinModelPortal:   B2UGQ0
- SMR:   B2UGQ0
- GeneID:   6287438
- GenomeReviews:   CP001068_GR
- KEGG:   rpi:Rpic_2030
- HOGENOM:   HBG398270
- OMA:   SDMAANT
- ProtClustDB:   CLSK896994
- GO:   GO:0005737
- HAMAP:   MF_01547
- InterPro:   IPR015507
- InterPro:   IPR002877
- PANTHER:   PTHR10920
- PIRSF:   PIRSF005461

Pfam domain/function: PF01728 FtsJ

EC number: =2.1.1.166

Molecular weight: Translated: 24506; Mature: 24375

Theoretical pI: Translated: 8.45; Mature: 8.45

Prosite motif: NA

Important sites: ACT_SITE 174-174 BINDING 60-60 BINDING 62-62 BINDING 89-89 BINDING 105-105 BINDING 134-134

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPG
CCCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCC
SWSQYVRNKLAASPRAKDGRIDGAIVAIDILPMEAIADVTFIQGDFREDEVFRQLEEIVL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECHHHHHHHHEECCCCCHHHHHHHHHHHHE
EVSGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFAMAHLKPEGALLIKCFHGSG
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
YSQIVEMFKRHFKIVAPRKPKASRDKSSETFLLGRQLKHPG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHCCCCC
>Mature Secondary Structure 
AKNKFNQSWLHDHINDPYVKLAQREGYRARAAYKLKEIDEQDKLIKPGQVIVDLGAAPG
CCCCCCHHHHHHCCCCHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCCEEEEECCCCCC
SWSQYVRNKLAASPRAKDGRIDGAIVAIDILPMEAIADVTFIQGDFREDEVFRQLEEIVL
HHHHHHHHHHHCCCCCCCCCCCCEEEEEEEECHHHHHHHHEECCCCCHHHHHHHHHHHHE
EVSGGGKVDLVLSDMAPNLSGVASADAARMEHIAELAVEFAMAHLKPEGALLIKCFHGSG
ECCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEECCCC
YSQIVEMFKRHFKIVAPRKPKASRDKSSETFLLGRQLKHPG
HHHHHHHHHHHHEEECCCCCCCCCCCCCCEEEEHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA