Click here to switch to the map view.

The map label for this gene is lpd [H]

Identifier: 187929024

GI number: 187929024

Start: 2049842

End: 2051623

Strand: Reverse

Name: lpd [H]

Synonym: Rpic_1944

Alternate gene names: 187929024

Gene position: 2051623-2049842 (Counterclockwise)

Preceding gene: 187929025

Following gene: 187929021

Centisome position: 52.04

GC content: 64.53

Gene sequence:

>1782_bases
ATGAGCGTGGTGGAAATCAAGGTGCCGGACATCGGCGATTTCGATGCGGTGGAAGTCATCGAAGTGCTGATCAAGGCGGG
CGATACCGTGGCGCCCGAGCAGTCGCTGATCGTGCTGGAATCCGACAAGGCCAGCATGGAAGTGCCGTCGGAGGTGGCTG
GCAAGATCGTCGACGTAAAAGTCAAGGTGGGCGACAAGGTCAGCAAGGGCACGGTGATCGCGACGGCGGAAGCTGGCGCG
GCTGCTGCTCCAGGACCTGCACAAGCGCCGGCTCCGGCCCCAGCACCGGCTGCCGCACCGGCAGCATCAGCCCCGGCACC
GCAAGCGGCCAAGCACGCCGGCGGCGCCGACATCGAGTGCGAGATGCTCGTCCTGGGCTCCGGCCCGGGCGGTTATTCGG
CCGCATTCCGCAGTGCGGATCTGGGCATGAACACTGTGCTGGTGGAGCGTTTCTCAACGCTGGGCGGCGTTTGCCTGAAC
GTGGGGTGTATCCCGTCGAAGGCGTTGCTGCACACGGCGGCCGTCATGGACGAAGTCAAGGCGATGGCCGCACACGGCAT
TGTCTACAGCGAGCCGACGGTTGATATCAACCAACTGCGCAAGCACAAGGAGTCCGTCATCGGCAAGCTGACCGGCGGGC
TGGCCGGCATGGCCAAGGCACGCAAGGTGCAGGTCGTGCGCGGTGTTGGCACGTTCCTGGATCCGAATCACCTCGAAGTG
CAACTGACGGACGGCGACGGCAAGGCAACGACGGGCGAGAAGAAGGTCATCCGTTTTGCCAAGGCGATCATCGCTGCAGG
CAGCGAGGCCGTGAAGCTGCCGTTCATTCCGGAAGATCCGCGCATCGTCGATTCGACCGGCGCGCTGGAGCTGCGCGAAG
TGCCGGGCAGGATGCTGGTCATCGGGGGCGGCATCATCGGCCTGGAAATGGCGACCGTGTACAGCACGCTGGGCGCCCGC
ATCGACGTGGTGGAAATGCTCGATGGCCTGATGCAGGGCGCTGACCGCGACCTCGTCAAGGTGTGGGACAAGATGAACAA
GAGCCGCTTCGACAAGGTCATGCTCAAGACCAAGACGGTCGGGGTCGAGGCCAAGCCGGACGGCATTTACGTCAAGTTCG
AGGGCGAAGCCGCACCTGCCGAGCCGCAACGCTATGACATGGTGCTGGTGGCCGTGGGCCGCACCCCGAACGGCAAGCGC
ATTGGCGCCGAGAAGGCCGGCGTGGCGGTGACCGACCGTGGCTTCATCGACGTGGATAAGCAACAGCGCACGAATGTGCC
GCACATCTTCGCGATCGGCGACCTCGTGGGGCAGCCGATGCTGGCACACAAGGCCGTGCATGAAGGTCACGTGGCGGCAG
AAGCCGCGCACGGCGAGAAGGCGTATTTCGATGCCAAGCAGATTCCGTCGGTCGCCTATACCGATCCGGAAGTGGCCTGG
GCAGGTCTGACCGAAGACCAGTGCAAGGCGCAGGGCATCAAGTACGGCAAGGGCGTGTTCCCGTGGGCCGCTTCGGGCCG
CGCGATTGCCAACGGCCGCGACGAAGGCTTCACGAAGTTGATCTTCGACGAGGAAACGCACCGCATCATCGGTGGCGGCA
TCGTTGGCACGCACGCCGGCGACTTGATCGGCGAAATCTGCCTGGCCATCGAGATGGGCGCGGACGCCGTGGACATCGGC
AAGACCATCCACCCGCACCCGACGCTGGGTGAGTCGGTGGGCATGGCTGCGGAAATCTACGAGGGTGTTTGCACGGACGT
GCCCCCGGCTCGTAAGCGATGA

Upstream 100 bases:

>100_bases
GGCGGTCCGATCGAAGTGCACACAGTACGTCTGCAGGATTGAAAAGCGTCAGCGGGCCCGGGCGATGCCGGGTGCCGACG
ACAAGAACAAGGAGTCAGGA

Downstream 100 bases:

>100_bases
ACTGCAATTAACCGCCAGAGTGTGGAGGCAACTCCGGATCTGGCTGGCAAACAAAAGGGCCGCTCGAATGGGCGGCCCTT
TTGTTTGGGATGATCGGCGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 593; Mature: 592

Protein sequence:

>593_residues
MSVVEIKVPDIGDFDAVEVIEVLIKAGDTVAPEQSLIVLESDKASMEVPSEVAGKIVDVKVKVGDKVSKGTVIATAEAGA
AAAPGPAQAPAPAPAPAAAPAASAPAPQAAKHAGGADIECEMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLN
VGCIPSKALLHTAAVMDEVKAMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGTFLDPNHLEV
QLTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPGRMLVIGGGIIGLEMATVYSTLGAR
IDVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRTPNGKR
IGAEKAGVAVTDRGFIDVDKQQRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAW
AGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLIGEICLAIEMGADAVDIG
KTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR

Sequences:

>Translated_593_residues
MSVVEIKVPDIGDFDAVEVIEVLIKAGDTVAPEQSLIVLESDKASMEVPSEVAGKIVDVKVKVGDKVSKGTVIATAEAGA
AAAPGPAQAPAPAPAPAAAPAASAPAPQAAKHAGGADIECEMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLN
VGCIPSKALLHTAAVMDEVKAMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGTFLDPNHLEV
QLTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPGRMLVIGGGIIGLEMATVYSTLGAR
IDVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRTPNGKR
IGAEKAGVAVTDRGFIDVDKQQRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAW
AGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLIGEICLAIEMGADAVDIG
KTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
>Mature_592_residues
SVVEIKVPDIGDFDAVEVIEVLIKAGDTVAPEQSLIVLESDKASMEVPSEVAGKIVDVKVKVGDKVSKGTVIATAEAGAA
AAPGPAQAPAPAPAPAAAPAASAPAPQAAKHAGGADIECEMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLNV
GCIPSKALLHTAAVMDEVKAMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGTFLDPNHLEVQ
LTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPGRMLVIGGGIIGLEMATVYSTLGARI
DVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTVGVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRTPNGKRI
GAEKAGVAVTDRGFIDVDKQQRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAWA
GLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAGDLIGEICLAIEMGADAVDIGK
TIHPHPTLGESVGMAAEIYEGVCTDVPPARKR

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=461, Percent_Identity=41.4316702819957, Blast_Score=322, Evalue=9e-88,
Organism=Homo sapiens, GI50301238, Length=475, Percent_Identity=26.1052631578947, Blast_Score=152, Evalue=1e-36,
Organism=Homo sapiens, GI291045266, Length=439, Percent_Identity=27.3348519362187, Blast_Score=130, Evalue=5e-30,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=25.9770114942529, Blast_Score=129, Evalue=9e-30,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=25.9770114942529, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=25.9770114942529, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=25.9770114942529, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=25.9770114942529, Blast_Score=129, Evalue=1e-29,
Organism=Homo sapiens, GI22035672, Length=444, Percent_Identity=27.9279279279279, Blast_Score=122, Evalue=8e-28,
Organism=Homo sapiens, GI291045268, Length=432, Percent_Identity=26.1574074074074, Blast_Score=112, Evalue=8e-25,
Organism=Escherichia coli, GI1786307, Length=478, Percent_Identity=65.2719665271967, Blast_Score=620, Evalue=1e-179,
Organism=Escherichia coli, GI87082354, Length=472, Percent_Identity=28.6016949152542, Blast_Score=181, Evalue=2e-46,
Organism=Escherichia coli, GI87081717, Length=460, Percent_Identity=25.8695652173913, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789915, Length=442, Percent_Identity=26.6968325791855, Blast_Score=136, Evalue=4e-33,
Organism=Escherichia coli, GI1786305, Length=73, Percent_Identity=57.5342465753425, Blast_Score=79, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI32565766, Length=458, Percent_Identity=40.174672489083, Blast_Score=321, Evalue=5e-88,
Organism=Caenorhabditis elegans, GI17557007, Length=476, Percent_Identity=26.890756302521, Blast_Score=133, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI71983429, Length=449, Percent_Identity=24.7216035634744, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71983419, Length=450, Percent_Identity=24.8888888888889, Blast_Score=117, Evalue=2e-26,
Organism=Caenorhabditis elegans, GI71982272, Length=455, Percent_Identity=25.9340659340659, Blast_Score=108, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6321091, Length=458, Percent_Identity=40.6113537117904, Blast_Score=305, Evalue=1e-83,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=27.6729559748428, Blast_Score=182, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6325166, Length=475, Percent_Identity=25.8947368421053, Blast_Score=136, Evalue=9e-33,
Organism=Drosophila melanogaster, GI21358499, Length=460, Percent_Identity=40.6521739130435, Blast_Score=330, Evalue=1e-90,
Organism=Drosophila melanogaster, GI24640551, Length=554, Percent_Identity=27.0758122743682, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24640553, Length=494, Percent_Identity=27.9352226720648, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24640549, Length=435, Percent_Identity=28.735632183908, Blast_Score=119, Evalue=8e-27,
Organism=Drosophila melanogaster, GI17737741, Length=484, Percent_Identity=25.4132231404959, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 61831; Mature: 61700

Theoretical pI: Translated: 5.65; Mature: 5.65

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVVEIKVPDIGDFDAVEVIEVLIKAGDTVAPEQSLIVLESDKASMEVPSEVAGKIVDVK
CCEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHCCCEEEEE
VKVGDKVSKGTVIATAEAGAAAAPGPAQAPAPAPAPAAAPAASAPAPQAAKHAGGADIEC
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEE
EMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLNVGCIPSKALLHTAAVMDEVK
EEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
AMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGTFLDPNHLEV
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCCCCEEE
QLTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPGRMLV
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCEEHCCCCCEEEE
IGGGIIGLEMATVYSTLGARIDVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTV
EECCEEHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHEEEEEE
GVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRTPNGKRIGAEKAGVAVTDRGFIDVDK
CEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEECCH
QQRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAW
HHCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHEECCCCCHHEECHHHCCCEEECCCCCEE
AGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAG
ECCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCHH
DLIGEICLAIEMGADAVDIGKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
SVVEIKVPDIGDFDAVEVIEVLIKAGDTVAPEQSLIVLESDKASMEVPSEVAGKIVDVK
CEEEEECCCCCCCCHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCHHHCCCEEEEE
VKVGDKVSKGTVIATAEAGAAAAPGPAQAPAPAPAPAAAPAASAPAPQAAKHAGGADIEC
EEECCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCEE
EMLVLGSGPGGYSAAFRSADLGMNTVLVERFSTLGGVCLNVGCIPSKALLHTAAVMDEVK
EEEEEECCCCCHHHHHHHCCCCCHHHHHHHHHHHCCEEEEECCCCCHHHHHHHHHHHHHH
AMAAHGIVYSEPTVDINQLRKHKESVIGKLTGGLAGMAKARKVQVVRGVGTFLDPNHLEV
HHHHCCEEECCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHEEEEECCCCCCCCCCEEE
QLTDGDGKATTGEKKVIRFAKAIIAAGSEAVKLPFIPEDPRIVDSTGALELREVPGRMLV
EEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCCCCCCCCEECCCCCEEHCCCCCEEEE
IGGGIIGLEMATVYSTLGARIDVVEMLDGLMQGADRDLVKVWDKMNKSRFDKVMLKTKTV
EECCEEHHHHHHHHHHHCCCEEHHHHHHHHHCCCCHHHHHHHHHHCHHHHHHHHEEEEEE
GVEAKPDGIYVKFEGEAAPAEPQRYDMVLVAVGRTPNGKRIGAEKAGVAVTDRGFIDVDK
CEEECCCEEEEEECCCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCEEEECCCCEECCH
QQRTNVPHIFAIGDLVGQPMLAHKAVHEGHVAAEAAHGEKAYFDAKQIPSVAYTDPEVAW
HHCCCCCEEEEEHHHHCCCHHHHHHHHCCCHHEECCCCCHHEECHHHCCCEEECCCCCEE
AGLTEDQCKAQGIKYGKGVFPWAASGRAIANGRDEGFTKLIFDEETHRIIGGGIVGTHAG
ECCCHHHHHHCCCCCCCCCCCCCCCCCEEECCCCCCCEEEEECCCCCEEEECCEECCCHH
DLIGEICLAIEMGADAVDIGKTIHPHPTLGESVGMAAEIYEGVCTDVPPARKR
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]